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BMRB: 6448

1XHS

Solution structure of the hypothetical protein ytfP from Escherichia coli. Northeast Structural Genomics target ER111.

Authors

Aramini, J.M., Huang, Y.J., Swapna, G.V.T., Paranji, R.K., Xiao, R., Shastry, R., Acton, T.B., Cort, J.R., Kennedy, M.A., Montelione, G.T.

Assembly

Hypothetical UPF0131 protein ytfP

Entity

1. Hypothetical UPF0131 protein ytfP (polymer, Thiol state: not present), 121 monomers, 13931.37 Da Detail

MRIFVYGSLR HKQGNSHWMT NAQLLGDFSI DNYQLYSLGH YPGAVPGNGT VHGEVYRIDN ATLAELDALR TRGGEYARQL IQTPYGSAWM YVYQRPVDGL KLIESGDWLD RDKLEHHHHH H

Formula weight

13931.37 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 90.9 %, Completeness (bb): 91.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.9 % (1285 of 1414)	91.1 % (666 of 731)	90.6 % (500 of 552)	90.8 % (119 of 131)
Backbone	91.4 % (656 of 718)	90.4 % (227 of 251)	92.6 % (324 of 350)	89.7 % (105 of 117)
Sidechain	89.7 % (721 of 804)	90.0 % (432 of 480)	88.7 % (275 of 310)	100.0 % (14 of 14)
Aromatic	75.0 % (126 of 168)	75.0 % (63 of 84)	74.1 % (60 of 81)	100.0 % (3 of 3)
Methyl	94.2 % (113 of 120)	93.3 % (56 of 60)	95.0 % (57 of 60)

1. Hypothetical UPF0131 protein ytfP

MRIFVYGSLR HKQGNSHWMT NAQLLGDFSI DNYQLYSLGH YPGAVPGNGT VHGEVYRIDN ATLAELDALR TRGGEYARQL IQTPYGSAWM YVYQRPVDGL KLIESGDWLD RDKLEHHHHH H

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1XHS, Strand ID: A Detail

Coupling constant

61 J values in 1 lists
Pressure 1 atm, Temperature 293 K, pH 5.5 Detail

Release date

2005-06-11

Citation

1H, 13C, and 15N Resonance Assignments for Escherichia coli ytfP, a Member of the Broadly Conserved UPF0131 Protein Domain Family
Aramini, J.M., Swapna, G.V.T., Huang, Y.J., Rajan, P.K., Xiao, R., Shastry, R., Acton, T.B., Cort, J.R., Kennedy, M.A., Montelione, G.T.
J. Biomol. NMR (2005), 33, 197-197, PubMed , DOI:

Related entities 1. Hypothetical UPF0131 protein ytfP, : 1 : 2 : 10 entities Detail

Experiments performed 19 experiments Detail

1 3D 15N-SEPARATED NOESY

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

2 3D 13C-SEPARATED NOESY (aliph)

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

3 4D 13C-SEPARATED NOESY

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

4 HNHA

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

5 HIGH RESOLUTION CH-HSQC

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

6 3D HNCO

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

7 HCCH TOCSY

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

8 HCCH COSY

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

9 3D 13C-SEPARATED NOESY (arom)

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

10 3D HNCOCACB

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

11 3D HNCACB

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

12 3D HNCOCA

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

13 3D HNCA

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

14 3D HNCACO

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

15 HCCONH

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

16 CCONH

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

17 HACANH

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

18 HACACONH

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

19 HBCBcgcdHD

Instrument

#1 Model Varian Inova (800 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (600 MHz)

#4 Model Varian Inova (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
2	NH4OAC		20 mM
3	CACL2		5 mM
4	NaCL		0.1 M
5	DTT		10 mM
6	NAN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
9	Hypothetical UPF0131 protein ytfP	[U-13C; U-15N]	1.3 mM
10	NH4OAC		20 mM
11	CACL2		5 mM
12	NaCL		0.1 M
13	DTT		10 mM
14	NAN3		0.02 %
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 5.5

#	Name	Isotope labeling	Concentration
16	Hypothetical UPF0131 protein ytfP	[U-5% 13C; U-15N]	1.1 mM
17	NH4OAC		20 mM
18	CACL2		5 mM
19	NaCL		0.1 M
20	DTT		10 mM
21	NAN3		0.02 %
22	D2O		5 %
23	H2O		95 %

nullKeywords ER111, NMR structure, AUTOSTRUCTURE, Northeast Structural Genomics Consortium, NESG, Protein Structure Initiative, PSI, Structural Genomics

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BMRB: 6448

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Related entities 1. Hypothetical UPF0131 protein ytfP, : 1 : 2 : 10 entities Detail

Experiments performed 19 experiments Detail

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