BMRB: 6454

SdiA

Authors

Yao, Y., Martinez-Yamout, M.A., Dyson, J.H.

Assembly

sdia

Entity

1. sdia (polymer, Thiol state: all free), 171 monomers, 20368.09 Da Detail

MSDKDFFSWR RTMLLRFQRM ETAEEVYHEI ELQAQQLEYD YYSLCVRHPV PFTRPKVAFY TNYPEAWVSY YQAKNFLAID PVLNPENFSQ GHLMWNDDLF SEAQPLWEAA RAHGLRRGVT QYLMLPERAL GFLSFSRCSA REIPILSDEL QLKMQLLVRE SLMALMRLND E

Formula weight

20368.09 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 82.0 %, Completeness (bb): 82.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.0 % (1727 of 2107)	87.2 % (960 of 1101)	73.8 % (608 of 824)	87.4 % (159 of 182)
Backbone	82.2 % (829 of 1008)	96.4 % (325 of 337)	68.6 % (349 of 509)	95.7 % (155 of 162)
Sidechain	83.3 % (1054 of 1266)	83.2 % (636 of 764)	85.9 % (414 of 482)	20.0 % (4 of 20)
Aromatic	78.0 % (184 of 236)	78.0 % (92 of 118)	77.2 % (88 of 114)	100.0 % (4 of 4)
Methyl	97.7 % (172 of 176)	98.9 % (87 of 88)	96.6 % (85 of 88)

1. sdia

MSDKDFFSWR RTMLLRFQRM ETAEEVYHEI ELQAQQLEYD YYSLCVRHPV PFTRPKVAFY TNYPEAWVSY YQAKNFLAID PVLNPENFSQ GHLMWNDDLF SEAQPLWEAA RAHGLRRGVT QYLMLPERAL GFLSFSRCSA REIPILSDEL QLKMQLLVRE SLMALMRLND E

Show sample condition

Sample

Temperature 293 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	sdia			0.3 mM
2	NaAc/HAc buffer			50 mM
3	D2O			100 %

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LACS Plot; CA

Referencing offset: 0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.01 ppm	external	direct	1.0
13C	DSS	methyl protons	0.01 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.01 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.01 ppm	external	direct	1.0
13C	DSS	methyl protons	0.01 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.01 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.01 ppm	external	direct	1.0
13C	DSS	methyl protons	0.01 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.01 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2AVX, Strand ID: A Detail

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Release date

2005-07-25

Citation

Backbone and side chain 1H, 13C and 15N assignments for Escherichia coli SdiA1-171, the autoinducer-binding domain of a quorum sensing protein
Yao, Y., Martinez-Yamout, M.A., Dyson, H.JANE.
J. Biomol. NMR (2005), 31, 373-374, PubMed 15929010 , DOI 10.1007/s10858-005-2470-0 , Abstract

Related entities 1. sdia, : 1 : 4 : 5 entities Detail

Interaction partners 1. sdia, : 5 interactors Detail

Experiments performed 2 experiments Detail