BMRBj - BMREntryMaster

Found 1 Document (3.566 seconds)

BMRB: 6456

1WA7

NMR STRUCTURE OF THE SH3 DOMAIN OF HUMAN LYN TYROSINE KINASE

Authors

Bauer, F., Schweimer, K., Hoffmann, S., Roesch, P., Sticht, H.

Assembly

SH3 domain of Lyn Tyrosine Kinase with a herpesviral ligand

Entity

1. SH3 domain of the Lyn kinase (polymer, Thiol state: not present), 68 monomers, 7630.591 Da Detail

GPLGSPEEQG DIVVALYPYD GIHPDDLSFK KGEKMKVLEE HGEWWKAKSL LTKKEGFIPS NYVAKLNT

2. Tyrosine kinase interacting protein (polymer, Thiol state: not present), 23 monomers, 2498.810 Da Detail

TWDPGMPTPP LPPRPANLGE RQA

Total weight

10129.4 Da

Max. entity weight

7630.591 Da

Source organism

Homo sapiens , Saimiriine gammaherpesvirus 2

Exptl. method

NMR

Refine. method

XPLOR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 75.8 %, Completeness: 69.2 %, Completeness (bb): 64.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.2 % (747 of 1079)	74.6 % (425 of 570)	60.2 % (254 of 422)	78.2 % (68 of 87)
Backbone	64.6 % (337 of 522)	77.7 % (139 of 179)	51.1 % (135 of 264)	79.7 % (63 of 79)
Sidechain	73.7 % (471 of 639)	73.1 % (286 of 391)	75.0 % (180 of 240)	62.5 % (5 of 8)
Aromatic	86.4 % (76 of 88)	86.4 % (38 of 44)	87.8 % (36 of 41)	66.7 % (2 of 3)
Methyl	78.0 % (64 of 82)	80.5 % (33 of 41)	75.6 % (31 of 41)

1. SH3 domain of the Lyn kinase

GPLGSPEEQG DIVVALYPYD GIHPDDLSFK KGEKMKVLEE HGEWWKAKSL LTKKEGFIPS NYVAKLNT

2. Tyrosine kinase interacting protein

TWDPGMPTPP LPPRPANLGE RQA

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Chem. Shift Complete2

Sequence coverage: 34.1 %, Completeness: 48.2 %, Completeness (bb): 45.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.2 % (1041 of 2158)	52.1 % (594 of 1140)	42.2 % (356 of 844)	52.3 % (91 of 174)
Backbone	45.6 % (476 of 1044)	53.9 % (193 of 358)	37.7 % (199 of 528)	53.2 % (84 of 158)
Sidechain	51.3 % (655 of 1278)	51.3 % (401 of 782)	51.5 % (247 of 480)	43.8 % (7 of 16)
Aromatic	43.2 % (76 of 176)	43.2 % (38 of 88)	43.9 % (36 of 82)	33.3 % (2 of 6)
Methyl	50.0 % (82 of 164)	51.2 % (42 of 82)	48.8 % (40 of 82)

1. SH3 domain of the Lyn kinase

GPLGSPEEQG DIVVALYPYD GIHPDDLSFK KGEKMKVLEE HGEWWKAKSL LTKKEGFIPS NYVAKLNT

2. Tyrosine kinase interacting protein

TWDPGMPTPP LPPRPANLGE RQA

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1WA7, Strand ID: A, B Detail

Release date

2005-10-16

Citation

Structural characterization of Lyn-SH3 domain in complex with a herpesviral protein reveals an extended recognition motif that enhances binding affinity
Bauer, F., Schweimer, K., Meiselbach, H., Hoffmann, S., Roesch, P., Sticht, H.
Protein Sci. (2005), 14, 2487-2498, PubMed 16155203 , DOI 10.1110/ps.051563605 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 6261 released on 2005-10-16
    Title NMR STRUCTURE OF THE SH3 DOMAIN OF HUMAN LYN TYROSINE KINASE
  Swiss-Prot: P07948 released on 1988-08-01
    Title LYN_HUMAN Entity Tyrosine-protein kinase Lyn

Related entities 1. SH3 domain of the Lyn kinase, : 2 : 164 entities Detail

Related entities 2. Tyrosine kinase interacting protein, : 1 : 1 : 1 : 1 entities Detail

Interaction partners 1. SH3 domain of the Lyn kinase, : 93 : 1 interactors Detail

More details for 94 interactors identified by 42 citations

Interaction partners 2. Tyrosine kinase interacting protein, : 2 : 1 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 9 experiments Detail

1 1H-13C NOESY

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker Avance (700 MHz)

#3 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

2 1H-15N NOESY

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker Avance (700 MHz)

#3 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

3 CBCACONH

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker Avance (700 MHz)

#3 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

4 HNCA

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker Avance (700 MHz)

#3 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

5 HNCACB

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker Avance (700 MHz)

#3 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

6 HNCO

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker Avance (700 MHz)

#3 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

7 CCONH

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker Avance (700 MHz)

#3 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

8 HNHA

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker Avance (700 MHz)

#3 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

9 HCCHTOCSY

Instrument

#1 Model Bruker Avance (800 MHz)

#2 Model Bruker Avance (700 MHz)

#3 Model Bruker Avance (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM
2	Tyrosine kinase interacting protein			3.4 mM

Sample #2

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	SH3 domain of the Lyn kinase	[U-99% 13C; U-98% 15N]		1.5 mM

Sample #3

Temperature 298 (±1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	SH3 domain of the Lyn kinase			3.3 mM
5	Tyrosine kinase interacting protein	[U-99% 13C; U-98% 15N]		1.5 mM

null

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Found 1 Document (3.566 seconds)

BMRB: 6456

Sample

Sample

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. SH3 domain of the Lyn kinase, : 2 : 164 entities Detail

Related entities 2. Tyrosine kinase interacting protein, : 1 : 1 : 1 : 1 entities Detail

Interaction partners 1. SH3 domain of the Lyn kinase, : 93 : 1 interactors Detail

Interaction partners 2. Tyrosine kinase interacting protein, : 2 : 1 interactors Detail

Experiments performed 9 experiments Detail

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