BMRB: 6464

Structure of the hypothetical Arabidopsis thaliana protein At1g16640.1

Authors

Peterson, F.C., Waltner, J.L., Lytle, B.L., Volkman, B.F.

Assembly

At1g16640

Entity

1. At1g16640 (polymer, Thiol state: all free), 104 monomers, 12165.76 Da Detail

GSMADTGEVQ FMKPFISEKS SKSLEIPLGF NEYFPAPFPI TVDLLDYSGR SWTVRMKKRG EKVFLTVGWE NFVKDNNLED GKYLQFIYDR DRTFYVIIYG HNMC

Formula weight

12165.76 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

Automated methods were used for backbone chemical shift assignment and iterative NOE refinement. Final structures were obtained by molecular dynamics in explicit solvent.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 77.5 %, Completeness (bb): 90.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.5 % (991 of 1279)	78.0 % (520 of 667)	74.2 % (374 of 504)	89.8 % (97 of 108)
Backbone	90.6 % (556 of 614)	91.0 % (192 of 211)	90.1 % (274 of 304)	90.9 % (90 of 99)
Sidechain	68.6 % (522 of 761)	71.9 % (328 of 456)	63.2 % (187 of 296)	77.8 % (7 of 9)
Aromatic	3.6 % (6 of 166)	4.8 % (4 of 83)	0.0 % (0 of 81)	100.0 % (2 of 2)
Methyl	97.9 % (92 of 94)	97.9 % (46 of 47)	97.9 % (46 of 47)

1. At1g16640

GSMADTGEVQ FMKPFISEKS SKSLEIPLGF NEYFPAPFPI TVDLLDYSGR SWTVRMKKRG EKVFLTVGWE NFVKDNNLED GKYLQFIYDR DRTFYVIIYG HNMC

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	At1g16640	[U-15N; U-13C]		0.8 mM
2	PO4			20 mM
3	NaCl			50 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.43 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.43 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.13 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1YEL, Strand ID: A Detail

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Release date

2005-10-06

Citation

Structure of the B3 domain from Arabidopsis thaliana protein At1g16640
Waltner, J.K., Peterson, F.C., Lytle, B.L., Volkman, B.F.
Protein Sci. (2005), 14, 2478-2483, PubMed 16081658 , DOI 10.1110/ps.051606305 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. At1g16640, : 1 : 2 : 80 entities Detail

Interaction partners 1. At1g16640, : 20 interactors Detail

Experiments performed 3 experiments Detail