BMRB: 6467

Solution structure of APETx2, a specific peptide inhibitor of ASIC3 proton-gated channels

Authors

Chagot, B., Escoubas, P., Diochot, S., Bernard, C., Lazdunski, M., Darbon, H.

Assembly

APETx2

Entity

1. APETx2 (polymer, Thiol state: all disulfide bound), 42 monomers, 4567.086 Da Detail

GTACSCGNSK GIYWFYRPSC PTDRGYTGSC RYFLGTCCTP AD

Formula weight

4567.086 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS37:SG
2	disulfide	sing	1:CYS6:SG	1:CYS30:SG
3	disulfide	sing	1:CYS20:SG	1:CYS38:SG

Source organism

Anthopleura elegantissima

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.3 %, Completeness (bb): 96.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	84.3 % (194 of 230)	84.3 % (194 of 230)
Backbone	96.6 % (84 of 87)	96.6 % (84 of 87)
Sidechain	76.9 % (110 of 143)	76.9 % (110 of 143)
Aromatic	43.8 % (14 of 32)	43.8 % (14 of 32)
Methyl	90.9 % (10 of 11)	90.9 % (10 of 11)

1. Anthopleura elegantissima toxin 2

GTACSCGNSK GIYWFYRPSC PTDRGYTGSC RYFLGTCCTP AD

Show sample condition

Sample

Temperature 280 (±0) K, pH 3.0 (±0)

#	Name	Isotope labeling	Type	Concentration
1	Anthopleura elegantissima toxin 2			1.32 mM

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Protein Blocks Logo

Calculated from 25 models in PDB: 1WXN, Strand ID: A Detail

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Release date

2005-01-20

Citation

Solution structure of APETx2, a specific peptide inhibitor of ASIC3 proton-gated channels
Chagot, B., Escoubas, P., Diochot, S., Bernard, C., Lazdunski, M., Darbon, H.
Protein Sci. (2005), 14, 2003-2010, PubMed 15987885 , DOI 10.1110/ps.051378905 , Abstract

Related entities 1. APETx2, : 1 : 2 : 26 entities Detail

Experiments performed 3 experiments Detail