BMRBj - BMREntryMaster

Found 1 Document (2.491 seconds)

BMRB: 6468

2LGC

1H, 13C and 15N backbone resonance assignment of p38 mitogen-activated protein kinase

Authors

Vogtherr, M., Saxena, K., Grimme, S., Betz, M., Schieborr, U., Pescatore, B., Langer, T., Schwalbe, H.

Assembly

mitogen-activated protein kinase p38 alpha

Entity

1. mitogen-activated protein kinase p38 alpha (polymer, Thiol state: all free), 351 monomers, 40210.51 Da Detail

GHNSQERPTF YRQELNKTIW EVPERYQNLS PVGSGAYGSV CAAFDTKTGH RVAVKKLSRP FQSIIHAKRT YRELRLLKHM KHENVIGLLD VFTPARSLEE FNDVYLVTHL MGADLNNIVK CQKLTDDHVQ FLIYQILRGL KYIHSADIIH RDLKPSNLAV NEDCELKILD FGLARHTDDE MTGYVATRWY RAPEIMLNWM HYNQTVDIWS VGCIMAELLT GRTLFPGTDH IDQLKLILRL VGTPGAELLK KISSESARNY IQSLAQMPKM NFANVFIGAN PLAVDLLEKM LVLDSDKRIT AAQALAHAYF AQYHDPDDEP VADPYDQSFE SRDLLIDEWK SLTYDEVISF V

Formula weight

40210.51 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics, sampling

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 76.4 %, Completeness: 37.8 %, Completeness (bb): 61.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.8 % (1576 of 4172)	25.0 % (540 of 2160)	48.4 % (794 of 1641)	65.2 % (242 of 371)
Backbone	61.6 % (1281 of 2078)	44.2 % (311 of 704)	70.3 % (729 of 1037)	71.5 % (241 of 337)
Sidechain	22.0 % (534 of 2429)	15.9 % (231 of 1456)	32.2 % (302 of 939)	2.9 % (1 of 34)
Aromatic	3.0 % (11 of 366)	5.5 % (10 of 183)	0.6 % (1 of 178)	0.0 % (0 of 5)
Methyl	24.1 % (103 of 428)	22.4 % (48 of 214)	25.7 % (55 of 214)

1. mitogen-activated protein kinase p38 alpha

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	mitogen-activated protein kinase p38 alpha	[U-2H; U-13C; U-15N]	0.7 mM
2	NaCl		150 mM
3	Hepes*HCl		50 mM
4	DTT		5 mM
5	TSP		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.87 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.87 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.36 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LGC, Strand ID: A Detail

Release date

2006-01-05

Citation

NMR backbone assignment of the mitogen-activated protein (MAP) kinase p38
Vogtherr, M., Saxena, K., Grimme, S., Betz, M., Schieborr, U., Pescatore, B., Langer, T., Schwalbe, H.
J. Biomol. NMR (2005), 32, 175-175, PubMed 16034669 , DOI 10.1007/s10858-005-2449-x , Abstract

Related entities 1. mitogen-activated protein kinase p38 alpha, : 94 : 206 entities Detail

Interaction partners 1. mitogen-activated protein kinase p38 alpha, : 28 interactors Detail

More details for 28 interactors identified by 14 citations

Experiments performed 5 experiments Detail

1 Deuterium-decoupled TROSY variants of HNCO

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	mitogen-activated protein kinase p38 alpha	[U-2H; U-13C; U-15N]	0.7 mM
2	NaCl		150 mM
3	Hepes*HCl		50 mM
4	DTT		5 mM
5	TSP		1 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	mitogen-activated protein kinase p38 alpha	selectively labeled		0.2 mM

2 HN(CA)CO

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	mitogen-activated protein kinase p38 alpha	[U-2H; U-13C; U-15N]	0.7 mM
2	NaCl		150 mM
3	Hepes*HCl		50 mM
4	DTT		5 mM
5	TSP		1 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	mitogen-activated protein kinase p38 alpha	selectively labeled		0.2 mM

3 HNCA

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	mitogen-activated protein kinase p38 alpha	[U-2H; U-13C; U-15N]	0.7 mM
2	NaCl		150 mM
3	Hepes*HCl		50 mM
4	DTT		5 mM
5	TSP		1 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	mitogen-activated protein kinase p38 alpha	selectively labeled		0.2 mM

4 HNCACB

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	mitogen-activated protein kinase p38 alpha	[U-2H; U-13C; U-15N]	0.7 mM
2	NaCl		150 mM
3	Hepes*HCl		50 mM
4	DTT		5 mM
5	TSP		1 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	mitogen-activated protein kinase p38 alpha	selectively labeled		0.2 mM

5 HN(CO)CACB

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Concentration
1	mitogen-activated protein kinase p38 alpha	[U-2H; U-13C; U-15N]	0.7 mM
2	NaCl		150 mM
3	Hepes*HCl		50 mM
4	DTT		5 mM
5	TSP		1 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	mitogen-activated protein kinase p38 alpha	selectively labeled		0.2 mM

null

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Found 1 Document (2.491 seconds)

BMRB: 6468

Sample

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Chemical shift reference

Chemical shift reference

Related entities 1. mitogen-activated protein kinase p38 alpha, : 94 : 206 entities Detail

Interaction partners 1. mitogen-activated protein kinase p38 alpha, : 28 interactors Detail

Experiments performed 5 experiments Detail

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