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BMRB: 6475

2BGP

1H, 13C and 15N Chemical Shift assignments for Man5C-CBM35 from C. japonicus

Authors

Tunnicliffe, R.B., Williamson, M.P.

Assembly

Man5C CBM35 Mannan Binding Module

Entity

1. Man5C CBM35 Mannan Binding Module (polymer, Thiol state: not present), 132 monomers, 13868.41 Da Detail

MAVPEGNSWT YTAASASITA PAQLVGNVGE LQGAGSAVIW NVDVPVTGEY RINLTWSSPY SSKVNTLVMD GTALSYAFAE ATVPVTYVQT KTLSAGNHSF GVRVGSSDWG YMNVHSLKLE LLGGLTIRSP AN

Formula weight

13868.41 Da

Source organism

Cellvibrio japonicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 94.4 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.4 % (1351 of 1431)	94.6 % (682 of 721)	94.2 % (536 of 569)	94.3 % (133 of 141)
Backbone	95.5 % (745 of 780)	95.9 % (260 of 271)	95.6 % (366 of 383)	94.4 % (119 of 126)
Sidechain	93.6 % (721 of 770)	93.8 % (422 of 450)	93.4 % (285 of 305)	93.3 % (14 of 15)
Aromatic	80.6 % (100 of 124)	82.3 % (51 of 62)	77.6 % (45 of 58)	100.0 % (4 of 4)
Methyl	98.8 % (170 of 172)	98.8 % (85 of 86)	98.8 % (85 of 86)

1. Man5C-CBM35

MAVPEGNSWT YTAASASITA PAQLVGNVGE LQGAGSAVIW NVDVPVTGEY RINLTWSSPY SSKVNTLVMD GTALSYAFAE ATVPVTYVQT KTLSAGNHSF GVRVGSSDWG YMNVHSLKLE LLGGLTIRSP AN

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Man5C-CBM35	[U-95% 13C; U-95% 15N]		1.0 mM
2	sodium phosphate			50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 1.01 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 5 models in PDB: 2BGO, Strand ID: A Detail

Release date

2005-05-26

Citation

Structure of a mannan-specific family 35 carbohydrate-binding module: evidence for significant conformational changes upon ligand binding
Tunnicliffe, R.B., Bolam, D.N., Pell, G., Gilbert, H.J., Williamson, M.P.
J. Mol. Biol. (2005), 347, 287-296, PubMed 15740741 , DOI 10.1016/j.jmb.2005.01.038 , Abstract

Related entities 1. Man5C CBM35 Mannan Binding Module, : 1 : 1 : 2 entities Detail

Experiments performed 10 experiments Detail

1 HSQC (15N and 13C)