BMRB: 6476

Full 1H, 13C and 15N Chemical Shift Assignments for the GII loop mutant domain (GII-L) of the yeast linker histone, Hho1p

Authors

Sanderson, A., Stott, K., Stevens, T.J., Thomas, J.O.

Assembly

GII loop mutant, GII-L

Entity

1. GII loop mutant, GII-L (polymer, Thiol state: all free), 87 monomers, 9537.083 Da Detail

KASSPSSLTY KEMILKSMPQ LNDGKGSSRI VLKKYVKDTY PIVGSASNFD YLFNSAIKKC VENGELVQPK GPSGIIKLNK KKVKLST

Formula weight

9537.083 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

see journal citation

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.8 %, Completeness (bb): 96.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.8 % (1002 of 1046)	95.3 % (527 of 553)	96.0 % (388 of 404)	97.8 % (87 of 89)
Backbone	96.7 % (495 of 512)	97.1 % (170 of 175)	96.1 % (245 of 255)	97.6 % (80 of 82)
Sidechain	95.4 % (587 of 615)	94.4 % (357 of 378)	97.0 % (223 of 230)	100.0 % (7 of 7)
Aromatic	80.8 % (42 of 52)	61.5 % (16 of 26)	100.0 % (26 of 26)
Methyl	97.8 % (90 of 92)	100.0 % (46 of 46)	95.7 % (44 of 46)

1. GII-L

KASSPSSLTY KEMILKSMPQ LNDGKGSSRI VLKKYVKDTY PIVGSASNFD YLFNSAIKKC VENGELVQPK GPSGIIKLNK KKVKLST

Show sample condition

Sample

Temperature 288 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	GII-L	[U-13C; U-15N]		1 mM
2	sodium phosphate buffer			100 mM
3	EDTA			1 mM
4	DTT	[U-2H]		1.0 ~ 2.0 mM

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LACS Plot; CA

Referencing offset: 0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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LACS Plot; CB

Referencing offset: 0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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LACS Plot; CO

Referencing offset: -1.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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Protein Blocks Logo

Calculated from 10 models in PDB: 1YQA, Strand ID: A Detail

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Release date

2005-09-21

Citation

Engineering the structural stability and functional properties of the GI domain into the intrinsically unfolded GII domain of the yeast linker histone Hho1p
Sanderson, A., Stott, K., Stevens, T.J., Thomas, J.O.
J. Mol. Biol. (2005), 349, 608-620, PubMed 15878177 , DOI 10.1016/j.jmb.2005.03.085 , Abstract

Related entities 1. GII loop mutant, GII-L, : 1 : 89 entities Detail

Interaction partners 1. GII loop mutant, GII-L, : 50 interactors Detail

Experiments performed 10 experiments Detail