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1YMO

Solution structure of the P2b-P3 pseudoknot from human telomerase RNA

Authors

Theimer, C.A., Blois, C.A., Feigon, J.

Assembly

Telomerase RNA P2b-P3 pseudoknot

Entity

1. Telomerase RNA P2b-P3 pseudoknot (polymer, Thiol state: not present), 47 monomers, 15045.89 Da Detail

GGGCUGUUUU UCUCGCUGAC UUUCAGCCCC AAACAAAAAA GUCAGCA

Formula weight

15045.89 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.1 %, Completeness (bb): 75.0 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	81.1 % (612 of 755)	76.8 % (318 of 414)	85.3 % (273 of 320)	100.0 % (21 of 21)
Suger, PO4	75.0 % (388 of 517)	69.5 % (196 of 282)	81.7 % (192 of 235)
Nucleobase	94.1 % (224 of 238)	92.4 % (122 of 132)	95.3 % (81 of 85)	100.0 % (21 of 21)
Aromatic	98.1 % (208 of 212)	100.0 % (106 of 106)	95.3 % (81 of 85)	100.0 % (21 of 21)

1. Telomerase RNA P2b-P3 pseudoknot

GGGCUGUUUU UCUCGCUGAC UUUCAGCCCC AAACAAAAAA GUCAGCA

Show sample condition

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Release date

2006-02-16

Citation

Structure of the human telomerase RNA pseudoknot reveals conserved tertiary interactions essential for function
Theimer, C.A., Blois, C.A., Feigon, J.
Mol. Cell (2005), 17, 671-682, PubMed 15749017 , DOI 10.1016/j.molcel.2005.01.017 , Abstract

Related entities 1. Telomerase RNA P2b-P3 pseudoknot, : 1 : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

1 2D NOESY (11echo and watergate)

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

2 2D NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

3 2D TOCSY (presat)

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

4 2D 15N-HMQC

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

5 2D 15N-CPMG-NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

6 2D JNN-HNN-COSY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

7 2D 13C-HSQC

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

8 2D HCCH COSY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

9 3D HCCH-TOCSY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

10 2D 13C filtered/edited NOESYs

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, pH 6.3

#	Name	Concentration
1	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, pH 6.3

#	Name	Concentration
8	Telomerase RNA P2b-P3 pseudoknot	0.8 mM
9	sodium phosphate buffer	10 mM
10	KCl	200 mM
11	EDTA	50 uM
12	sodium azide	0.2 %
13	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
14	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
15	sodium phosphate buffer		10 mM
16	KCl		200 mM
17	EDTA		50 uM
18	sodium azide		0.2 %
19	H2O		95 %
20	D2O		5 %

Sample #4

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
21	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]	0.8 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

Sample #5

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
27	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
28	sodium phosphate buffer		10 mM
29	KCl		200 mM
30	EDTA		50 uM
31	sodium azide		0.2 %
32	H2O		95 %
33	D2O		5 %

Sample #6

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
34	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-A	0.8 mM
35	sodium phosphate buffer		10 mM
36	KCl		200 mM
37	EDTA		50 uM
38	sodium azide		0.2 %
39	D2O		100 %

Sample #7

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
40	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
41	sodium phosphate buffer		10 mM
42	KCl		200 mM
43	EDTA		50 uM
44	sodium azide		0.2 %
45	H2O		95 %
46	D2O		5 %

Sample #8

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
47	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-C	0.8 mM
48	sodium phosphate buffer		10 mM
49	KCl		200 mM
50	EDTA		50 uM
51	sodium azide		0.2 %
52	D2O		100 %

Sample #9

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
53	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
54	sodium phosphate buffer		10 mM
55	KCl		200 mM
56	EDTA		50 uM
57	sodium azide		0.2 %
58	H2O		95 %
59	D2O		5 %

Sample #10

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
60	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-G	0.8 mM
61	sodium phosphate buffer		10 mM
62	KCl		200 mM
63	EDTA		50 uM
64	sodium azide		0.2 %
65	D2O		100 %

Sample #11

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
66	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
67	sodium phosphate buffer		10 mM
68	KCl		200 mM
69	EDTA		50 uM
70	sodium azide		0.2 %
71	H2O		95 %
72	D2O		5 %

Sample #12

Pressure 1 atm, pH 6.3

#	Name	Isotope labeling	Concentration
73	Telomerase RNA P2b-P3 pseudoknot	[U-13C; U-15N]-U	0.8 mM
74	sodium phosphate buffer		10 mM
75	KCl		200 mM
76	EDTA		50 uM
77	sodium azide		0.2 %
78	D2O		100 %

nullKeywords Hoogsteen, non-canonical, pseudoknot, tertiary structure, triplex

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Link destinations for BMRB, PDB and EMDB

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BMRB: 6477

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Related entities 1. Telomerase RNA P2b-P3 pseudoknot, : 1 : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

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