BMRBj - BMREntryMaster

Found 1 Document (2.077 seconds)

BMRB: 6480

1YJV

The loss of stability of a naturally occurring ATP7A mutant is the cause of Menkes disease

Authors

Banci, L., Bertini, I., Cantini, F., Migliardi, M., Rosato, A., Wang, S.

Assembly

Copper-transporting ATPase 1(E.C.3.6.3.4)

Entity

1. Copper-transporting ATPase 1(E.C.3.6.3.4) (polymer, Thiol state: free and other bound), 75 monomers, 8179.493 Da Detail

MGDGVLELVV RGMTCASCVH KIESSLTKHR GILYCSVALA TNKAHIKYDP EIIGPRDIIH TIESLGFEAS LVKIE

2. CU1 (non-polymer), 63.546 Da

Total weight

8243.039 Da

Max. entity weight

8179.493 Da

Entity Connection

na 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:CU11:CU
2	na	sing	1:CYS18:SG	2:CU11:CU

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics coupled with simulated annealing followed by restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 87.6 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	87.6 % (446 of 509)	86.4 % (376 of 435)	94.6 % (70 of 74)
Backbone	94.7 % (215 of 227)	94.8 % (146 of 154)	94.5 % (69 of 73)
Sidechain	81.9 % (231 of 282)	81.9 % (230 of 281)	100.0 % (1 of 1)
Aromatic	52.4 % (11 of 21)	52.4 % (11 of 21)
Methyl	92.5 % (49 of 53)	92.5 % (49 of 53)

1. Copper-transporting ATPase 1(E.C.3.6.3.4)

MGDGVLELVV RGMTCASCVH KIESSLTKHR GILYCSVALA TNKAHIKYDP EIIGPRDIIH TIESLGFEAS LVKIE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Protein Blocks Logo

Calculated from 30 models in PDB: 1YJV, Strand ID: A Detail

Release date

2005-11-13

Citation

An atomic-level investigation of the disease-causing A629P mutant of the Menkes protein, ATP7A
Banci, L., Bertini, I., Cantini, F., Migliardi, M., Rosato, A., Wang, S.
J. Mol. Biol. (2005), 352, 409-417, PubMed 16083905 , DOI 10.1016/j.jmb.2005.07.034 , Abstract

Related entities 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 1 : 1 : 4 : 356 entities Detail

Interaction partners 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 8 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1(E.C.3.6.3.4)	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

2 CBCANH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1(E.C.3.6.3.4)	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

3 CBCACONH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1(E.C.3.6.3.4)	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

4 HNCO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1(E.C.3.6.3.4)	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

5 HNCACO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1(E.C.3.6.3.4)	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

6 2D NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1(E.C.3.6.3.4)	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

7 2D TOCSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1(E.C.3.6.3.4)	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

8 HNHA

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1(E.C.3.6.3.4)	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1(E.C.3.6.3.4)	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

nullKeywords copper(I), metal homeostasis, metallochaperone, protein-protein interaction

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Found 1 Document (2.077 seconds)

BMRB: 6480

Sample

Related entities 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 1 : 1 : 4 : 356 entities Detail

Interaction partners 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 5 interactors Detail

Experiments performed 8 experiments Detail

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