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Found 1 Document (2.0 seconds)

BMRB: 6481

1YJU

The loss of stability of a naturally occurring ATP7A mutant is the cause of Menkes disease

Authors

Banci, L., Bertini, I., Cantini, F., Migliardi, M., Rosato, A., Wang, S.

Assembly

Copper-transporting ATPase 1(E.C.3.6.3.4)

Entity

1. Copper-transporting ATPase 1(E.C.3.6.3.4) (polymer, Thiol state: all free), 75 monomers, 8179.493 Da Detail

MGDGVLELVV RGMTCASCVH KIESSLTKHR GILYCSVALA TNKAHIKYDP EIIGPRDIIH TIESLGFEAS LVKIE

Formula weight

8179.493 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics coupled with simulated annealing followed by restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 81.7 %, Completeness (bb): 91.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	81.7 % (416 of 509)	80.0 % (348 of 435)	91.9 % (68 of 74)
Backbone	91.6 % (208 of 227)	91.6 % (141 of 154)	91.8 % (67 of 73)
Sidechain	73.8 % (208 of 282)	73.7 % (207 of 281)	100.0 % (1 of 1)
Aromatic	42.9 % (9 of 21)	42.9 % (9 of 21)
Methyl	96.2 % (51 of 53)	96.2 % (51 of 53)

1. Copper-transporting ATPase 1

MGDGVLELVV RGMTCASCVH KIESSLTKHR GILYCSVALA TNKAHIKYDP EIIGPRDIIH TIESLGFEAS LVKIE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Protein Blocks Logo

Calculated from 30 models in PDB: 1YJU, Strand ID: A Detail

Release date

2005-11-13

Citation

An atomic-level investigation of the disease-causing A629P mutant of the Menkes protein, ATP7A
Banci, L., Bertini, I., Cantini, F., Migliardi, M., Rosato, A., Wang, S.
J. Mol. Biol. (2005), 352, 409-417, PubMed 16083905 , DOI 10.1016/j.jmb.2005.07.034 , Abstract

Related entities 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 1 : 1 : 4 : 356 entities Detail

Interaction partners 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 8 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

2 CBCANH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

3 CBCACONH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

4 HNCO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

5 HNCACO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

6 2D NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

7 2D TOCSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

8 HNHA

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

nullKeywords copper(I), metal homeostasis, metallochaperone, protein-protein interaction

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Found 1 Document (2.0 seconds)

BMRB: 6481

Sample

Related entities 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 1 : 1 : 4 : 356 entities Detail

Interaction partners 1. Copper-transporting ATPase 1(E.C.3.6.3.4), : 5 interactors Detail

Experiments performed 8 experiments Detail

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