BMRBj - BMREntryMaster

Found 1 Document (1.978 seconds)

BMRB: 6482

1YJT

The loss of stability of a naturally occurring ATP7A mutant is the cause of Menkes disease

Authors

Banci, L., Bertini, I., Cantini, F., Migliardi, M., Rosato, A., Wang, S.

Assembly

Copper-transporting ATPase 1(E.C.3.6.3.4)

Entity

1. Copper-transporting ATPase 1 (polymer, Thiol state: free and other bound), 75 monomers, 8205.530 Da Detail

MGDGVLELVV RGMTCASCVH KIESSLTKHR GILYCSVALA TNKAHIKYDP EIIGPRDIIH TIESLGFEPS LVKIE

2. CU1 (non-polymer), 63.546 Da

Total weight

8269.076 Da

Max. entity weight

8205.53 Da

Entity Connection

na 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:CU11:CU
2	na	sing	1:CYS18:SG	2:CU11:CU

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics coupled with simulated annealing followed by restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 87.1 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	87.1 % (446 of 512)	85.4 % (375 of 439)	97.3 % (71 of 73)
Backbone	96.4 % (217 of 225)	96.1 % (147 of 153)	97.2 % (70 of 72)
Sidechain	79.8 % (229 of 287)	79.7 % (228 of 286)	100.0 % (1 of 1)
Aromatic	57.1 % (12 of 21)	57.1 % (12 of 21)
Methyl	100.0 % (52 of 52)	100.0 % (52 of 52)

1. Copper-transporting ATPase 1

MGDGVLELVV RGMTCASCVH KIESSLTKHR GILYCSVALA TNKAHIKYDP EIIGPRDIIH TIESLGFEPS LVKIE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Protein Blocks Logo

Calculated from 30 models in PDB: 1YJT, Strand ID: A Detail

Release date

2005-11-13

Citation

An atomic-level investigation of the disease-causing A629P mutant of the Menkes protein, ATP7A
Banci, L., Bertini, I., Cantini, F., Migliardi, M., Rosato, A., Wang, S.
J. Mol. Biol. (2005), 352, 409-417, PubMed 16083905 , DOI 10.1016/j.jmb.2005.07.034 , Abstract

Related entities 1. Copper-transporting ATPase 1, : 1 : 1 : 4 : 346 entities Detail

Interaction partners 1. Copper-transporting ATPase 1, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 8 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

2 CBCANH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

3 CBCACONH

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

4 HNCO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

5 HNCACO

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

6 2D NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

7 2D TOCSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

8 HNHA

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Copper-transporting ATPase 1	[U-15N]	0.8 mM
2	DTT		5 mM
3	phosphate buffer		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
6	Copper-transporting ATPase 1	[U-15N; U-13C]	0.8 mM
7	DTT		5 mM
8	phosphate buffer		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Concentration
11	Copper-transporting ATPase 1	1.0 mM
12	DTT	5 mM
13	phosphate buffer	100 mM
14	H2O	90 %
15	D2O	10 %

nullKeywords copper(I), metal homeostasis, metallochaperone, protein-protein interaction

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.978 seconds)

BMRB: 6482

Sample

Related entities 1. Copper-transporting ATPase 1, : 1 : 1 : 4 : 346 entities Detail

Interaction partners 1. Copper-transporting ATPase 1, : 5 interactors Detail

Experiments performed 8 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3