BMRBj - BMREntryMaster

1WU0

Chemical shift assignments for subunit c of the F1Fo ATP Synthase from Bacillus PS3

Authors

Nakano, T., Ikegami, T., Suzuki, T., Yoshida, M., Akutsu, H.

Assembly

F1Fo-ATP synthase subunit c

Entity

1. F1Fo-ATP synthase subunit c (polymer, Thiol state: not present), 72 monomers, 7333.745 Da Detail

MSLGVLAAAI AVGLGALGAG IGNGLIVSRT IEGIARQPEL RPVLQTTMFI GVALVEALPI IGVVFSFIYL GR

Formula weight

7333.745 Da

Source organism

Bacillus sp. PS3

Exptl. method

NMR

Refine. method

simulated annealing torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 93.7 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.7 % (748 of 798)	93.1 % (375 of 403)	94.7 % (306 of 323)	93.1 % (67 of 72)
Backbone	96.0 % (409 of 426)	94.1 % (143 of 152)	97.1 % (199 of 205)	97.1 % (67 of 69)
Sidechain	91.9 % (398 of 433)	92.4 % (232 of 251)	92.7 % (166 of 179)	0.0 % (0 of 3)
Aromatic	84.2 % (32 of 38)	84.2 % (16 of 19)	84.2 % (16 of 19)
Methyl	96.2 % (127 of 132)	97.0 % (64 of 66)	95.5 % (63 of 66)

1. F1Fo-ATP synthase subunit c

MSLGVLAAAI AVGLGALGAG IGNGLIVSRT IEGIARQPEL RPVLQTTMFI GVALVEALPI IGVVFSFIYL GR

Temperature 298 (±0.01) K, pH 2 (±0.1)

#	Name	Isotope labeling	Concentration
1	F1Fo-ATP synthase subunit c		2 mM
2	CDCl3	[U-2D]	75 %
3	CD3OH	[U-2D]	25 %

Temperature 298 (±0.01) K, pH 2 (±0.1)

#	Name	Isotope labeling	Concentration
4	F1Fo-ATP synthase subunit c	[U-15N]	2 mM
5	CDCl3	[U-2D]	75 %
6	CD3OH	[U-2D]	25 %

Temperature 298 (±0.01) K, pH 2 (±0.1)

#	Name	Isotope labeling	Concentration
7	F1Fo-ATP synthase subunit c	[U-13C; U-15N]	2 mM
8	CDCl3	[U-2D]	75 %
9	CD3OH	[U-2D]	25 %

Protein Blocks Logo

Calculated from 30 models in PDB: 1WU0, Strand ID: A Detail

Release date

2006-04-23

Citation

A new solution structure of ATP synthase subunit c from thermophilic Bacillus PS3, suggesting a local conformational change for H+-translocation
Nakano, T., Ikegami, T., Suzuki, T., Yoshida, M., Akutsu, H.
J. Mol. Biol. (2006), 358, 132-144, PubMed 16497328 , DOI 10.1016/j.jmb.2006.01.011 , Abstract

1 15N-1N-HSQC