BMRBj - BMREntryMaster

Found 1 Document (7.722 seconds)

BMRB: 6498

1YSM

Backbone and side-chain 1H, 13C, and 15N Chemical Shift Assignments for SIP (1-77)

Authors

Bhattacharya, S., Lee, Y., Michowski, W., Filipek, A., Kuznicki, J., Chazin, W.J.

Assembly

Siah-Interacting Protein (Residues 1-77)

Entity

1. Siah-Interacting Protein (Residues 1-77) (polymer, Thiol state: not present), 77 monomers, 8771.068 Da Detail

MASVLEELQK DLEEVKVLLE KSTRKRLRDT LTSEKSKIET ELKNKMQQKS QKKPELDNEK PAAVVAPLTT GYTVKIS

Formula weight

8771.068 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

Torsion Angle Dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 93.6 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.6 % (875 of 935)	94.4 % (469 of 497)	91.9 % (329 of 358)	96.2 % (77 of 80)
Backbone	94.7 % (432 of 456)	95.4 % (145 of 152)	93.9 % (216 of 230)	95.9 % (71 of 74)
Sidechain	93.0 % (516 of 555)	93.9 % (324 of 345)	91.2 % (186 of 204)	100.0 % (6 of 6)
Aromatic	25.0 % (2 of 8)	50.0 % (2 of 4)	0.0 % (0 of 4)
Methyl	91.5 % (86 of 94)	91.5 % (43 of 47)	91.5 % (43 of 47)

1. single chain biopolymer

MASVLEELQK DLEEVKVLLE KSTRKRLRDT LTSEKSKIET ELKNKMQQKS QKKPELDNEK PAAVVAPLTT GYTVKIS

Show sample condition

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	single chain biopolymer	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaPi		20.0 mM
3	NaCl		50 mM
4	H2O		90 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	single chain biopolymer	[U-100% 13C]	1 mM
6	NaPi		20 mM
7	NaCl		50 mM
8	D2O		100 %

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
9	single chain biopolymer	[U-10% 13C]	1 mM
10	NaPi		20 mM
11	NaCl		50 mM
12	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.101329
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.101329
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.101329
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.101329
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1YSM, Strand ID: A Detail

Release date

2005-08-15

Citation

The modular structure of SIP facilitates its role in stabilizing multiprotein assemblies
Bhattacharya, S., Lee, Y., Michowski, W., Filipek, A., Kuznicki, J., Chazin, W.J.
Biochemistry (2005), 44, 9462-9471, PubMed 15996101 , DOI 10.1021/bi0502689 , Abstract

Related entities 1. Siah-Interacting Protein (Residues 1-77), : 1 : 2 : 11 entities Detail

Interaction partners 1. Siah-Interacting Protein (Residues 1-77), : 20 interactors Detail

More details for 20 interactors identified by 4 citations

Experiments performed 8 experiments Detail

1 1H-15N HSQC

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	single chain biopolymer	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaPi		20.0 mM
3	NaCl		50 mM
4	H2O		90 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	single chain biopolymer	[U-100% 13C]	1 mM
6	NaPi		20 mM
7	NaCl		50 mM
8	D2O		100 %

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
9	single chain biopolymer	[U-10% 13C]	1 mM
10	NaPi		20 mM
11	NaCl		50 mM
12	D2O		100 %

2 1H-13C CT-HSQC

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	single chain biopolymer	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaPi		20.0 mM
3	NaCl		50 mM
4	H2O		90 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	single chain biopolymer	[U-100% 13C]	1 mM
6	NaPi		20 mM
7	NaCl		50 mM
8	D2O		100 %

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
9	single chain biopolymer	[U-10% 13C]	1 mM
10	NaPi		20 mM
11	NaCl		50 mM
12	D2O		100 %

3 3D-HNCO

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	single chain biopolymer	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaPi		20.0 mM
3	NaCl		50 mM
4	H2O		90 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	single chain biopolymer	[U-100% 13C]	1 mM
6	NaPi		20 mM
7	NaCl		50 mM
8	D2O		100 %

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
9	single chain biopolymer	[U-10% 13C]	1 mM
10	NaPi		20 mM
11	NaCl		50 mM
12	D2O		100 %

4 3D-HNCACB

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	single chain biopolymer	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaPi		20.0 mM
3	NaCl		50 mM
4	H2O		90 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	single chain biopolymer	[U-100% 13C]	1 mM
6	NaPi		20 mM
7	NaCl		50 mM
8	D2O		100 %

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
9	single chain biopolymer	[U-10% 13C]	1 mM
10	NaPi		20 mM
11	NaCl		50 mM
12	D2O		100 %

5 3D-CBCA(CO)NH

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	single chain biopolymer	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaPi		20.0 mM
3	NaCl		50 mM
4	H2O		90 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	single chain biopolymer	[U-100% 13C]	1 mM
6	NaPi		20 mM
7	NaCl		50 mM
8	D2O		100 %

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
9	single chain biopolymer	[U-10% 13C]	1 mM
10	NaPi		20 mM
11	NaCl		50 mM
12	D2O		100 %

6 3D-HC(CCO)NH

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	single chain biopolymer	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaPi		20.0 mM
3	NaCl		50 mM
4	H2O		90 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	single chain biopolymer	[U-100% 13C]	1 mM
6	NaPi		20 mM
7	NaCl		50 mM
8	D2O		100 %

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
9	single chain biopolymer	[U-10% 13C]	1 mM
10	NaPi		20 mM
11	NaCl		50 mM
12	D2O		100 %

7 3D-(H)CC(CO)NH

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	single chain biopolymer	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaPi		20.0 mM
3	NaCl		50 mM
4	H2O		90 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	single chain biopolymer	[U-100% 13C]	1 mM
6	NaPi		20 mM
7	NaCl		50 mM
8	D2O		100 %

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
9	single chain biopolymer	[U-10% 13C]	1 mM
10	NaPi		20 mM
11	NaCl		50 mM
12	D2O		100 %

8 3D-HCCH-TOCSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	single chain biopolymer	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaPi		20.0 mM
3	NaCl		50 mM
4	H2O		90 %

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	single chain biopolymer	[U-100% 13C]	1 mM
6	NaPi		20 mM
7	NaCl		50 mM
8	D2O		100 %

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
9	single chain biopolymer	[U-10% 13C]	1 mM
10	NaPi		20 mM
11	NaCl		50 mM
12	D2O		100 %

null

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Found 1 Document (7.722 seconds)

BMRB: 6498

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Siah-Interacting Protein (Residues 1-77), : 1 : 2 : 11 entities Detail

Interaction partners 1. Siah-Interacting Protein (Residues 1-77), : 20 interactors Detail

Experiments performed 8 experiments Detail

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