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Found 1 Document (2.343 seconds)

BMRB: 6509

1YG3

Solution Structure of the ScYLV P1-P2 Frameshifting Pseudoknot

Authors

Cornish, P.V., Hennig, M., Giedroc, D.P.

Assembly

ScYLV RNA pseudoknot

Entity

1. ScYLV RNA pseudoknot (polymer, Thiol state: not present), 31 monomers, 10051.06 Da Detail

AAAGUGGXGC CGACCACUUA AAAACACCGG A

Formula weight

10051.06 Da

Source organism

Sugarcane yellow leaf virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 92.2 %, Completeness (bb): 92.2 % Detail

Polymer type: polyribonucleotide

	Total	¹³C	¹⁵N	³¹P
All	92.2 % (224 of 243)	92.6 % (188 of 203)	90.0 % (9 of 10)	90.0 % (27 of 30)
Suger, PO4	92.2 % (166 of 180)	92.7 % (139 of 150)		90.0 % (27 of 30)
Nucleobase	92.1 % (58 of 63)	92.5 % (49 of 53)	90.0 % (9 of 10)
Aromatic	92.1 % (58 of 63)	92.5 % (49 of 53)	90.0 % (9 of 10)

1. ScYLV RNA pseudoknot

AAAGUGGXGC CGACCACUUA AAAACACCGG A

Show sample condition

Sample

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
1	ScYLV RNA pseudoknot	2 mM
2	KCl	100 mM
3	MgCl2	5 mM
4	H2O	90 %
5	D2O	10 %

Release date

2005-09-07

Citation

A loop 2 cytidine-stem 1 minor groove interaction as a positive determinant for pseudoknot-stimulated -1 ribosomal frameshifting
Cornish, P.V., Hennig, M., Giedroc, D.P.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 12694-12699, PubMed 16123125 , DOI 10.1073/pnas.0506166102 , Abstract

Related entities 1. ScYLV RNA pseudoknot, : 1 : 3 entities Detail

Experiments performed 6 experiments Detail

1 2D NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
1	ScYLV RNA pseudoknot	2 mM
2	KCl	100 mM
3	MgCl2	5 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
6	ScYLV RNA pseudoknot	2 mM
7	KCl	100 mM
8	MgCl2	5 mM
9	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
10	ScYLV RNA pseudoknot	2 mM
11	KCl	100 mM
12	MgCl2	5 mM
13	PF1 phage	12.5 mg/mL
14	D2O	100 %

2 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
1	ScYLV RNA pseudoknot	2 mM
2	KCl	100 mM
3	MgCl2	5 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
6	ScYLV RNA pseudoknot	2 mM
7	KCl	100 mM
8	MgCl2	5 mM
9	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
10	ScYLV RNA pseudoknot	2 mM
11	KCl	100 mM
12	MgCl2	5 mM
13	PF1 phage	12.5 mg/mL
14	D2O	100 %

3 CT-TROSY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
1	ScYLV RNA pseudoknot	2 mM
2	KCl	100 mM
3	MgCl2	5 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
6	ScYLV RNA pseudoknot	2 mM
7	KCl	100 mM
8	MgCl2	5 mM
9	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
10	ScYLV RNA pseudoknot	2 mM
11	KCl	100 mM
12	MgCl2	5 mM
13	PF1 phage	12.5 mg/mL
14	D2O	100 %

4 CT-antiTROSY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
1	ScYLV RNA pseudoknot	2 mM
2	KCl	100 mM
3	MgCl2	5 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
6	ScYLV RNA pseudoknot	2 mM
7	KCl	100 mM
8	MgCl2	5 mM
9	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
10	ScYLV RNA pseudoknot	2 mM
11	KCl	100 mM
12	MgCl2	5 mM
13	PF1 phage	12.5 mg/mL
14	D2O	100 %

5 J-modulated HSQC

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
1	ScYLV RNA pseudoknot	2 mM
2	KCl	100 mM
3	MgCl2	5 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
6	ScYLV RNA pseudoknot	2 mM
7	KCl	100 mM
8	MgCl2	5 mM
9	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
10	ScYLV RNA pseudoknot	2 mM
11	KCl	100 mM
12	MgCl2	5 mM
13	PF1 phage	12.5 mg/mL
14	D2O	100 %

6 2D TOCSY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
1	ScYLV RNA pseudoknot	2 mM
2	KCl	100 mM
3	MgCl2	5 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
6	ScYLV RNA pseudoknot	2 mM
7	KCl	100 mM
8	MgCl2	5 mM
9	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Concentration
10	ScYLV RNA pseudoknot	2 mM
11	KCl	100 mM
12	MgCl2	5 mM
13	PF1 phage	12.5 mg/mL
14	D2O	100 %

nullKeywords protonated cytidine, ribosomal frameshifting, RNA pseudoknot

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Found 1 Document (2.343 seconds)

BMRB: 6509

Sample

Related entities 1. ScYLV RNA pseudoknot, : 1 : 3 entities Detail

Experiments performed 6 experiments Detail

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