BMRB: 6510

Structure and inter-domain interactions of domain II from the blood stage malarial protein, apical membrane antigen 1

Authors

Feng, Z., Keizer, D.W., Stevenson, R.A., Yao, S., Babon, J.J., Murphy, V.J., Anders, R.F., Norton, R.S.

Assembly

AMA1 Domain II

Entity

1. AMA1 Domain II (polymer, Thiol state: all disulfide bound), 140 monomers, 15956.64 Da Detail

MRGSHHHHHH GSNAKFGLWV DGNCEDIPHV NEFPAIDLFE CNKLVFELSA SDQPKQYEQH LTDYEKIKEG FKNKNASMIK SAFLPTGAFK ADRYKSHGKG YNWGNYNTET QKCEIFNVKP TCLINNSSYI ATTALSHPIE

Formula weight

15956.64 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS24:SG	1:CYS122:SG
2	disulfide	sing	0:CYS41:SG	1:CYS113:SG

Source organism

Plasmodium falciparum

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 75.8 %, Completeness (bb): 93.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.8 % (1254 of 1655)	73.2 % (631 of 862)	75.8 % (486 of 641)	90.1 % (137 of 152)
Backbone	93.5 % (774 of 828)	94.3 % (267 of 283)	92.7 % (381 of 411)	94.0 % (126 of 134)
Sidechain	62.8 % (602 of 958)	62.9 % (364 of 579)	62.9 % (227 of 361)	61.1 % (11 of 18)
Aromatic	18.2 % (35 of 192)	34.4 % (33 of 96)	0.0 % (0 of 94)	100.0 % (2 of 2)
Methyl	82.1 % (92 of 112)	89.3 % (50 of 56)	75.0 % (42 of 56)

1. Apical membrane antigen ! Domain II

MRGSHHHHHH GSNAKFGLWV DGNCEDIPHV NEFPAIDLFE CNKLVFELSA SDQPKQYEQH LTDYEKIKEG FKNKNASMIK SAFLPTGAFK ADRYKSHGKG YNWGNYNTET QKCEIFNVKP TCLINNSSYI ATTALSHPIE

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Apical membrane antigen ! Domain II	[U-95% 13C; U-90% 15N]		0.1 ~ 0.8 mM

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1YXE, Strand ID: A Detail

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Release date

2005-08-21

Citation

Structure and inter-domain interactions of domain II from the blood-stage malarial protein, apical membrane antigen 1
Feng, Z., Keizer, D.W., Stevenson, R.A., Yao, S., Babon, J.J., Murphy, V.J., Anders, R.F., Norton, R.S.
J. Mol. Biol. (2005), 350, 641-656, PubMed 15964019 , DOI 10.1016/j.jmb.2005.05.011 , Abstract

Related entities 1. AMA1 Domain II, : 1 : 21 : 24 entities Detail