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BMRB: 6519

1YX7

Chemical shift assignments of Calsensin

Authors

Venkitaramani, D.V., Fulton, D.BRUCE., Andreotti, A.H., Johansen, K.M., Johansen, J.

Assembly

Calsensin

Entity

1. Calsensin (polymer, Thiol state: not reported), 83 monomers, 9101.513 Da Detail

MACKVKAELE AAFKKLDANG DGYVTALELQ TFMVTLDAYK ALSKDKVKEA SAKLIKMADK NSDGKISKEE FLNANAELLC QLK

Formula weight

9101.513 Da

Source organism

Haemopis marmorata

Exptl. method

Refine. method

Distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.6 %, Completeness: 70.5 %, Completeness (bb): 76.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.5 % (684 of 970)	74.5 % (376 of 505)	60.6 % (228 of 376)	89.9 % (80 of 89)
Backbone	76.5 % (381 of 498)	89.3 % (151 of 169)	63.0 % (155 of 246)	90.4 % (75 of 83)
Sidechain	68.1 % (376 of 552)	67.0 % (225 of 336)	69.5 % (146 of 210)	83.3 % (5 of 6)
Aromatic	32.6 % (15 of 46)	65.2 % (15 of 23)	0.0 % (0 of 23)
Methyl	80.0 % (80 of 100)	84.0 % (42 of 50)	76.0 % (38 of 50)

1. Calsensin

MACKVKAELE AAFKKLDANG DGYVTALELQ TFMVTLDAYK ALSKDKVKEA SAKLIKMADK NSDGKISKEE FLNANAELLC QLK

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

LACS Plot; CA

Referencing offset: -0.64 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.64 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1YX8, Strand ID: A Detail

Release date

2005-07-24

Citation

Solution structure and backbone dynamics of Calsensin, an invertebrate neuronal calcium-binding protein
Venkitaramani, D.V., Fulton, D.BRUCE., Andreotti, A.H., Johansen, K.H., Johansen, J.
Protein Sci. (2005), 14, 1894-1901, PubMed 15937283 , DOI 10.1110/ps.051412605 , Abstract

Related entities 1. Calsensin, : 1 : 2 : 241 entities Detail

Experiments performed 11 experiments Detail

1 3D 1H-15N NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

2 3D 1H-15N TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

3 3D 1H-13C NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

4 3D 1H-13N HCCH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

5 3D 1H-13C-15N HNCACB

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

6 3D 1H-13C-15N HN(CO)CACB

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

7 3D 15N-15N-1H NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

8 2D 1H-15NHSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

9 2D 1H-1H NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

10 2D 1H-1H COSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

11 2D 1H-1H TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Calsensin	[U-95% 13C; U-98% 15N]	protein	1.7 mM
2	Sodium chloride		salt	75 mM
3	Sodium Phosphate		salt	50 mM
4	Dithiothreotol			2 mM

nullKeywords calcium-binding protein, Calsensin, EF-hand, helix-loop-helix, nervous system