BMRB: 6520

1H, 15N Chemical shift assignments for recombinant elicitor protein PcF from the oomycete pathogen P. cactorum

Authors

Nicastro, G., Orsomando, G., Desario, F., Ferrari, E., Manconi, L., Spisni, A., Ruggeri, S.

Assembly

Pcf monomer

Entity

1. Pcf monomer (polymer, Thiol state: all disulfide bound), 52 monomers, 5612.136 Da Detail

EDPLYCQAIG CPTLYSEANL AVSKECRDQG KLGDDFHRCC EEQCGSTTPA SA

Formula weight

5612.136 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS40:SG
2	disulfide	sing	1:CYS11:SG	1:CYS44:SG
3	disulfide	sing	1:CYS26:SG	1:CYS39:SG

Source organism

Komagataella pastoris

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.0 %, Completeness (bb): 98.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	97.0 % (328 of 338)	97.5 % (278 of 285)	94.3 % (50 of 53)
Backbone	98.7 % (152 of 154)	98.1 % (103 of 105)	100.0 % (49 of 49)
Sidechain	95.7 % (176 of 184)	97.2 % (175 of 180)	25.0 % (1 of 4)
Aromatic	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	100.0 % (20 of 20)	100.0 % (20 of 20)

1. elicitor protein Pcf from the oomycete pathogen P. cactorum

EDPLYCQAIG CPTLYSEANL AVSKECRDQG KLGDDFHRCC EEQCGSTTPA SA

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Sample

Temperature 293 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pcf monomer	[U-15N]		0.65 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2BIC, Strand ID: A Detail

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Release date

2009-05-26

Citation 1

The solution structure of the recombinant elicitor protein PcF from the oomycete pathogen P. cactorum displays a helix-loop-helix fold and reveals calcium binding ability
Nicastro, G., Orsomando, G., Desario, F., Ferrari, E., Manconi, L., Spisni, A., Ruggeri, S.

Show more 1 citaion

Citation 2

PcF protein from Phytophthora cactorum and its recombinant homologue elicit phenylalanine ammonia lyase activation in tomato
Orsomando, G., Lorenzi, M., Ferrari, E., de Chiara, C., Spisni, A., Ruggeri, S.
Cell. Mol. Life Sci. (2003), 60, 1470-1476, PubMed 12943233 , DOI 10.1007/s00018-003-3079-7 , Abstract

Hide non-primary 1 citaion

Related entities 1. Pcf monomer, : 1 : 3 entities Detail

Experiments performed 5 experiments Detail