BMRB: 6527

1H, 13C and 15N Chemical Shift Assignments for km23 dimer

Authors

Ilangovan, U., Ding, W., Groppe, J.C., Trbovich, J.A., Zuniga, J., Demeler, B., Mulder, K., Hinck, A.P.

Assembly

Km23 homodimer

Entity

1. Km23 homodimer (polymer, Thiol state: not present), 96 monomers, 10921.47 × 2 Da Detail

MAEVEETLKR LQSQKGVQGI IVVNTEGIPI KSTMDNPTTT QYASLMHSFI LKARSTVRDI DPQNDLTFLR IRSKKNEIMV APDKDYFLIV IQNPTE

Total weight

21842.94 Da

Max. entity weight

10921.47 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.6 %, Completeness (bb): 99.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.6 % (1120 of 1147)	96.2 % (577 of 600)	99.6 % (443 of 445)	98.0 % (100 of 102)
Backbone	99.1 % (561 of 566)	98.9 % (188 of 190)	99.6 % (284 of 285)	97.8 % (89 of 91)
Sidechain	96.1 % (648 of 674)	94.9 % (389 of 410)	98.0 % (248 of 253)	100.0 % (11 of 11)
Aromatic	96.0 % (48 of 50)	96.0 % (24 of 25)	96.0 % (24 of 25)
Methyl	96.7 % (118 of 122)	96.7 % (59 of 61)	96.7 % (59 of 61)

1. Homedimeric Km23

MAEVEETLKR LQSQKGVQGI IVVNTEGIPI KSTMDNPTTT QYASLMHSFI LKARSTVRDI DPQNDLTFLR IRSKKNEIMV APDKDYFLIV IQNPTE

Show sample condition

Sample

Temperature 315 (±0.2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Homedimeric Km23	[U-13 C; U-15N]		1 mM

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 1Z09, Strand ID: A, B Detail

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Coupling constant

65 J values in 1 lists
Temperature 315 (±0.2) K, pH 6.0 (±0.05) Detail

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Temperature 315 (±0.2) K, pH 6.0 (±0.05)

List #1 J_coupling_list_1

Release date

2005-10-31

Citation

Structure and dynamics of the homodimeric dynein light chain km23
Ilangovan, U., Ding, W., Zhong, Y., Wilson, C.L., Groppe, J.C., Trbovich, J.T., Zuniga, J., Demeler, B., Tang, Q., Gao, G., Mulder, K.M., Hinck, A.P.
J. Mol. Biol. (2005), 352, 338-354, PubMed 16083906 , DOI 10.1016/j.jmb.2005.07.002 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Km23 homodimer, : 1 : 21 : 3 : 56 entities Detail

Interaction partners 1. Km23 homodimer, : 32 interactors Detail

Experiments performed 5 experiments Detail