BMRBj - BMREntryMaster

Found 1 Document (1.586 seconds)

BMRB: 6530

1YMZ

CC45, An Artificial WW Domain Designed Using Statistical Coupling Analysis

Authors

Socolich, M., Lockless, S.W., Russ, W.P., Lee, H., Gardner, K.H., Ranganathan, R.

Assembly

CC45

Entity

1. CC45 (polymer, Thiol state: not present), 43 monomers, 5047.661 Da Detail

GSHGRSMPLP PGWERRTDVE GKVYYFNVRT LTTTWERPTI ILE

Formula weight

5047.661 Da

Exptl. method

NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.7 %, Completeness: 82.6 %, Completeness (bb): 80.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	82.6 % (257 of 311)	82.5 % (222 of 269)	83.3 % (35 of 42)
Backbone	80.0 % (100 of 125)	79.1 % (68 of 86)	82.1 % (32 of 39)
Sidechain	84.4 % (157 of 186)	84.2 % (154 of 183)	100.0 % (3 of 3)
Aromatic	93.1 % (27 of 29)	92.6 % (25 of 27)	100.0 % (2 of 2)
Methyl	100.0 % (22 of 22)	100.0 % (22 of 22)

1. CC45

GSHGRSMPLP PGWERRTDVE GKVYYFNVRT LTTTWERPTI ILE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	CC45	600 uM
2	sodium phosphate buffer	100 mM
3	NaCl	100 mM
4	H2O	90 %
5	D2O	10 %

Protein Blocks Logo

Calculated from 10 models in PDB: 1YMZ, Strand ID: A Detail

Release date

2005-10-25

Citation

Evolutionary information for specifying a protein fold
Socolich, M., Lockless, S.W., Russ, W.P., Lee, H., Gardner, K.H., Ranganathan, R.
Nature (2005), 437, 512-518, PubMed 16177782 , DOI 10.1038/nature03991 , Abstract

Related entities 1. CC45, : 1 : 120 entities Detail

Experiments performed 5 experiments Detail

1 DQF-COSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	CC45	600 uM
2	sodium phosphate buffer	100 mM
3	NaCl	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
6	CC45	600 uM
7	sodium phosphate buffer	100 mM
8	NaCl	100 mM
9	D2O	99 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	CC45	[U-15N]	850 uM
11	sodium phosphate buffer		100 mM
12	NaCl		100 mM
13	H2O		90 %
14	D2O		10 %

2 2D TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	CC45	600 uM
2	sodium phosphate buffer	100 mM
3	NaCl	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
6	CC45	600 uM
7	sodium phosphate buffer	100 mM
8	NaCl	100 mM
9	D2O	99 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	CC45	[U-15N]	850 uM
11	sodium phosphate buffer		100 mM
12	NaCl		100 mM
13	H2O		90 %
14	D2O		10 %

3 2D NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	CC45	600 uM
2	sodium phosphate buffer	100 mM
3	NaCl	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
6	CC45	600 uM
7	sodium phosphate buffer	100 mM
8	NaCl	100 mM
9	D2O	99 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	CC45	[U-15N]	850 uM
11	sodium phosphate buffer		100 mM
12	NaCl		100 mM
13	H2O		90 %
14	D2O		10 %

4 3D 15N-separated NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	CC45	600 uM
2	sodium phosphate buffer	100 mM
3	NaCl	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
6	CC45	600 uM
7	sodium phosphate buffer	100 mM
8	NaCl	100 mM
9	D2O	99 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	CC45	[U-15N]	850 uM
11	sodium phosphate buffer		100 mM
12	NaCl		100 mM
13	H2O		90 %
14	D2O		10 %

5 3D 15N-separated TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
1	CC45	600 uM
2	sodium phosphate buffer	100 mM
3	NaCl	100 mM
4	H2O	90 %
5	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Concentration
6	CC45	600 uM
7	sodium phosphate buffer	100 mM
8	NaCl	100 mM
9	D2O	99 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	CC45	[U-15N]	850 uM
11	sodium phosphate buffer		100 mM
12	NaCl		100 mM
13	H2O		90 %
14	D2O		10 %

nullKeywords ARTIFICIAL PROTEIN, COMPUTATIONAL DESIGN

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Found 1 Document (1.586 seconds)

BMRB: 6530

Sample

Related entities 1. CC45, : 1 : 120 entities Detail

Experiments performed 5 experiments Detail

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