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BMRB: 6540

2AXL

Sequence-specific 1H, 13C and 15N resonance assignments of the winged-helix domain of the human Werner syndrome protein

Authors

Sun, J., Feng, H., Lin, G., Zeng, W., Hu, J.

Assembly

Werner syndrome protein

Entity

1. Werner syndrome protein (polymer, Thiol state: all free), 144 monomers, 16333.45 Da Detail

MDDSEDTSWD FGPQAFKLLS AVDILGEKFG IGLPILFLRG SNSQRLADQY RRHSLFGTGK DQTESWWKAF SRQLITGGFL VEVSRYNKFM KICALTKKGR NWLHKANTES QSLILQANEE LCPKKLLLPS SKTVSSGTKE HCYN

Formula weight

16333.45 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.8 %, Completeness (bb): 94.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.8 % (1395 of 1727)	72.9 % (658 of 903)	88.9 % (593 of 667)	91.7 % (144 of 157)
Backbone	94.9 % (812 of 856)	92.5 % (273 of 295)	97.6 % (411 of 421)	91.4 % (128 of 140)
Sidechain	71.0 % (713 of 1004)	63.5 % (386 of 608)	82.1 % (311 of 379)	94.1 % (16 of 17)
Aromatic	51.2 % (84 of 164)	51.2 % (42 of 82)	48.7 % (38 of 78)	100.0 % (4 of 4)
Methyl	88.7 % (126 of 142)	90.1 % (64 of 71)	87.3 % (62 of 71)

1. The winged-helix domain of WRN

MDDSEDTSWD FGPQAFKLLS AVDILGEKFG IGLPILFLRG SNSQRLADQY RRHSLFGTGK DQTESWWKAF SRQLITGGFL VEVSRYNKFM KICALTKKGR NWLHKANTES QSLILQANEE LCPKKLLLPS SKTVSSGTKE HCYN

Show sample condition

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	indirect	1.0
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	indirect	1.0
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	indirect	1.0
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	indirect	1.0
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2AXL, Strand ID: A Detail

Release date

2005-08-28

Citation 1

NMR assignments of the winged-helix domain of human werner syndrome protein
Sun, J., Feng, H., Lin, G., Zeng, W., Hu, J.
J. Biomol. NMR (2005), 32, 261-261, PubMed 16132834 , DOI 10.1007/s10858-005-7960-6 , Abstract

Show more 6 citaions

Citation 2

Positional cloning of the Werner's syndrome gene
Yu, C.E., Oshima, J., Fu, Y.H., Wijsman, E.M., Hisama, F., Alisch, R., Matthews, S., Nakura, J., Miki, T., Ouais, S., Martin, G.M., Mulligan, J., Schellenberg, G.D.
Science (1996), 272, 258-262, PubMed 8602509 , Abstract

Citation 3

RecQ helicases: suppressors of tumorigenesis and premature aging
Bachrati, C.Z., Hickson, I.D.
Biochem. J. (2003), 374, 577-606, PubMed 12803543 , DOI 10.1042/BJ20030491 , Abstract

Citation 4

A putative nucleic acid-binding domain in Bloom's and Werner's syndrome helicases
Morozov, V., Mushegian, A.R., Koonin, E.V., Bork, P.
Trends. Biochem. Sci. (1997), 22, 417-418, PubMed 9397680 , Abstract

Citation 5

Structure and function of RecQ DNA helicases
Bennett, R.J., Keck, J.L.
Crit. Rev. Biochem. Mol. Biol. (2004), 39, 79-97, PubMed 15217989 , DOI 10.1080/10409230490460756 , Abstract

Citation 6

Pathways and functions of the Werner syndrome protein
Lee, J.W., Harrigan, J., Opresko, P.L., Bohr, V.A.
Mech. Ageing Dev. (2005), 126, 79-86, PubMed 15610765 , DOI 10.1016/j.mad.2004.09.011 , Abstract

Citation 7

Werner helicase is localized to transcriptionally active nucleoli of cycling cells
Gray, M.D., Wang, L., Youssoufian, H., Martin, G.M., Oshima, J.
Exp. Cell Res. (1998), 242, 487-494, PubMed 9683536 , DOI 10.1006/excr.1998.4124 , Abstract

Hide non-primary 6 citaions

Related entities 1. Werner syndrome protein, : 1 : 3 : 7 entities Detail

Interaction partners 1. Werner syndrome protein, : 32 interactors Detail

More details for 32 interactors identified by 22 citations

Experiments performed 21 experiments Detail

1 1H-15N HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

2 HNCACB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

3 CBCA(CO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

4 HNCO

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

5 HNCOCO

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

6 HNCG_arom

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

7 HN(CO)CG_arom

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

8 HNCG

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

9 HN(CO)C

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

10 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

11 HBHA(CO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

12 DIPSI-H(CCO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

13 DIPSI-C(CCO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

14 HAHB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

15 HCCH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

16 HCCH-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

17 1H-13C HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

18 15N-HOHAHA-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

19 15N-NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

20 13C-15N NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

21 13C-13C NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
1	The winged-helix domain of WRN	[U-95% 15N]	0.45 mM
2	potassium phosphate buffer		25 mM
3	NaCl		25 mM
4	EDTA		1 mM
5	NaZ3		0.05 %

Sample #2

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
6	The winged-helix domain of WRN	[U-98% 15N]	0.45 mM
7	potassium phosphate buffer		25 mM
8	NaCl		25 mM
9	EDTA		1 mM
10	NaZ3		0.05 %

Sample #3

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Isotope labeling	Concentration
11	The winged-helix domain of WRN	[U-95% 13C; U-95% 15N]	0.45 mM
12	potassium phosphate buffer		25 mM
13	NaCl		25 mM
14	EDTA		1 mM
15	NaZ3		0.05 %

Sample #4

Temperature 295 (±1) K, pH 7.36 (±0.2)

#	Name	Concentration
16	The winged-helix domain of WRN	0.29 mM
17	potassium phosphate buffer	25 mM
18	NaCl	25 mM
19	EDTA	1 mM
20	NaZ3	0.05 %

nullKeywords NMR assignments, Werner syndrome protein, WRN, nucleolar targeting sequence, RQC domain, winged-helix, WH domain

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Found 1 Document (2.181 seconds)

BMRB: 6540

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Werner syndrome protein, : 1 : 3 : 7 entities Detail

Interaction partners 1. Werner syndrome protein, : 32 interactors Detail

Experiments performed 21 experiments Detail

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