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Found 1 Document (1.341 seconds)

BMRB: 6543

1Z2J

HIV-1 frameshift inducing element RNA

Authors

Staple, D.W., Butcher, S.E.

Assembly

HIV-1 frameshift inducing RNA

Entity

1. HIV-1 frameshift inducing RNA (polymer, Thiol state: not present), 45 monomers, 14582.68 Da Detail

GGGAAGAUCU GGCCUUCCCA CAAGGGAAGG CCAGGGAAUC UUCCC

Formula weight

14582.68 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 54.0 %, Completeness (bb): 38.2 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	54.0 % (386 of 715)	72.0 % (283 of 393)	30.2 % (91 of 301)	57.1 % (12 of 21)
Suger, PO4	38.2 % (189 of 495)	62.6 % (169 of 270)	8.9 % (20 of 225)
Nucleobase	89.5 % (197 of 220)	92.7 % (114 of 123)	93.4 % (71 of 76)	57.1 % (12 of 21)
Aromatic	92.3 % (179 of 194)	99.0 % (96 of 97)	93.4 % (71 of 76)	57.1 % (12 of 21)

1. HIV-1 frameshift induciting element RNA

GGGAAGAUCU GGCCUUCCCA CAAGGGAAGG CCAGGGAAUC UUCCC

Show sample condition

Sample

Pressure 1 (±0.1) atm, Temperature 303 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Release date

2006-11-05

Citation

Solution structure and thermodynamic investigation of the HIV-1 frameshift inducing element
Staple, D.W., Butcher, S.E.
J. Mol. Biol. (2005), 349, 1011-1023, PubMed 15927637 , DOI 10.1016/j.jmb.2005.03.038 , Abstract

Related entities 1. HIV-1 frameshift inducing RNA, : 2 : 1 : 1 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-1H NOESY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Varian Inova (800 MHz)

#4 Model Varian Inova (900 MHz)

Sample #1

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Sample #2

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-G,C	1 mM
6	NaCl		50 mM
7	D2O		100 %

2 2D 1H-1H TOCSY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Varian Inova (800 MHz)

#4 Model Varian Inova (900 MHz)

Sample #1

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Sample #2

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-G,C	1 mM
6	NaCl		50 mM
7	D2O		100 %

3 2D 1H-13C HSQC

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Varian Inova (800 MHz)

#4 Model Varian Inova (900 MHz)

Sample #1

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Sample #2

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-G,C	1 mM
6	NaCl		50 mM
7	D2O		100 %

4 2D 1H-15N HMQC

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Varian Inova (800 MHz)

#4 Model Varian Inova (900 MHz)

Sample #1

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Sample #2

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-G,C	1 mM
6	NaCl		50 mM
7	D2O		100 %

5 2D HNN COSY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Varian Inova (800 MHz)

#4 Model Varian Inova (900 MHz)

Sample #1

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Sample #2

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-G,C	1 mM
6	NaCl		50 mM
7	D2O		100 %

6 3D HCCH TOCSY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Varian Inova (800 MHz)

#4 Model Varian Inova (900 MHz)

Sample #1

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Sample #2

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-G,C	1 mM
6	NaCl		50 mM
7	D2O		100 %

7 3D HCCH COSY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Varian Inova (800 MHz)

#4 Model Varian Inova (900 MHz)

Sample #1

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Sample #2

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-G,C	1 mM
6	NaCl		50 mM
7	D2O		100 %

8 3D NOESY-HMQC

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Varian Inova (800 MHz)

#4 Model Varian Inova (900 MHz)

Sample #1

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Sample #2

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-G,C	1 mM
6	NaCl		50 mM
7	D2O		100 %

9 Filter/Edit 2D 1H-1H NOEY

Instrument

#1 Model Bruker DMX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Varian Inova (800 MHz)

#4 Model Varian Inova (900 MHz)

Sample #1

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-A,U	1 mM
2	NaCl		50 mM
3	D2O		10 %
4	H2O		90 %

Sample #2

Pressure 1 (±0.1) atm, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	HIV-1 frameshift induciting element RNA	[U-13C; U-15N]-G,C	1 mM
6	NaCl		50 mM
7	D2O		100 %

nullKeywords frameshift, HIV

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Found 1 Document (1.341 seconds)

BMRB: 6543

Sample

Related entities 1. HIV-1 frameshift inducing RNA, : 2 : 1 : 1 entities Detail

Experiments performed 9 experiments Detail

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