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Found 1 Document (3.015 seconds)

BMRB: 6551

2AQ0

Solution structure of the C-terminal domain of ERCC1 complexed with the C-terminal domain of XPF

Authors

Tripsianes, K., Folkers, G., Odijk, H., Jaspers, N.G., Hoeijmakers, J.H., Kaptein, R., Boelens, R.

Assembly

DNA excision repair protein ERCC-1, DNA repair endonuclease XPF (E.C.3.1.-.-)

Entity

1. ERCC-1 (polymer, Thiol state: all free), 89 monomers, 9984.341 Da Detail

MADLLMEKLE QDFVSRVTEC LTTVKSVNKT DSQTLLTTFG SLEQLIAASR EDLALCPGLG PQKARRLFDV LHEPFLKVPG GLEHHHHHH

2. XPF (polymer, Thiol state: all free), 84 monomers, 9217.408 Da Detail

MDSETLPESE KYNPGPQDFL LKMPGVNAKN CRSLMHHVKN IAELAALSQD ELTSILGNAA NAKQLYDFIH TSFAEVVSKG KGKK

Total weight

19201.75 Da

Max. entity weight

9984.341 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

water refinment in CNS

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 56.6 %, Completeness: 48.3 %, Completeness (bb): 45.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.3 % (968 of 2005)	52.4 % (546 of 1042)	42.5 % (333 of 784)	49.7 % (89 of 179)
Backbone	45.2 % (462 of 1022)	52.9 % (184 of 348)	38.1 % (194 of 509)	50.9 % (84 of 165)
Sidechain	51.6 % (591 of 1146)	52.2 % (362 of 694)	51.1 % (224 of 438)	35.7 % (5 of 14)
Aromatic	34.1 % (43 of 126)	34.9 % (22 of 63)	33.3 % (21 of 63)
Methyl	59.6 % (118 of 198)	59.6 % (59 of 99)	59.6 % (59 of 99)

1. ERCC-1

MADLLMEKLE QDFVSRVTEC LTTVKSVNKT DSQTLLTTFG SLEQLIAASR EDLALCPGLG PQKARRLFDV LHEPFLKVPG GLEHHHHHH

2. XPF

MDSETLPESE KYNPGPQDFL LKMPGVNAKN CRSLMHHVKN IAELAALSQD ELTSILGNAA NAKQLYDFIH TSFAEVVSKG KGKK

Show sample condition

Sample

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	ERCC-1	[U-15N; U-13C]	1.5 mM
2	XPF	[U-15N; U-13C]	1.5 mM
3	phosphate		50 mM
4	NaCl		100 mM
5	D2O		8 %
6	H2O		92 %

Chem. Shift Complete2

Sequence coverage: 59.0 %, Completeness: 48.4 %, Completeness (bb): 45.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.4 % (1941 of 4010)	52.4 % (1092 of 2084)	41.9 % (657 of 1568)	53.6 % (192 of 358)
Backbone	45.9 % (939 of 2044)	53.9 % (375 of 696)	38.0 % (387 of 1018)	53.6 % (177 of 330)
Sidechain	50.7 % (1162 of 2292)	51.2 % (710 of 1388)	50.0 % (438 of 876)	50.0 % (14 of 28)
Aromatic	34.5 % (87 of 252)	35.7 % (45 of 126)	33.3 % (42 of 126)
Methyl	54.5 % (216 of 396)	55.6 % (110 of 198)	53.5 % (106 of 198)

1. ERCC-1

MADLLMEKLE QDFVSRVTEC LTTVKSVNKT DSQTLLTTFG SLEQLIAASR EDLALCPGLG PQKARRLFDV LHEPFLKVPG GLEHHHHHH

2. XPF

MDSETLPESE KYNPGPQDFL LKMPGVNAKN CRSLMHHVKN IAELAALSQD ELTSILGNAA NAKQLYDFIH TSFAEVVSKG KGKK

Show sample condition

Sample

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	ERCC-1	[U-15N; U-13C]	1.5 mM
2	XPF	[U-15N; U-13C]	1.5 mM
3	phosphate		50 mM
4	NaCl		100 mM
5	D2O		8 %
6	H2O		92 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1Z00, Strand ID: A, B Detail

Release date

2005-12-13

Citation

The structure of the human ERCC1/XPF interaction domains reveals a complementary role for the two proteins in nucleotide excision repair
Tripsianes, K., Folkers, G., AB, E., Das, D., Odijk, H., Jaspers, N.G.J., Hoeijmakers, J.H.J., Kaptein, R., Boelens, R.
Structure (2005), 13, 1849-1858, PubMed 16338413 , DOI 10.1016/j.str.2005.08.014 , Abstract

Related entities 1. ERCC-1, : 1 : 4 : 14 entities Detail

Related entities 2. XPF, : 1 : 2 : 2 : 13 entities Detail

Interaction partners 1. ERCC-1, : 21 : 1 interactors Detail

More details for 22 interactors identified by 10 citations

Interaction partners 2. XPF, : 24 : 1 interactors Detail

More details for 25 interactors identified by 10 citations

Experiments performed 6 experiments Detail

1 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	ERCC-1	[U-15N; U-13C]	1.5 mM
2	XPF	[U-15N; U-13C]	1.5 mM
3	phosphate		50 mM
4	NaCl		100 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	ERCC-1	[U-15N]	1 mM
8	XPF	[U-15N]	1 mM
9	phosphate		50 mM
10	NaCl		100 mM
11	D2O		8 %
12	H2O		92 %

2 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	ERCC-1	[U-15N; U-13C]	1.5 mM
2	XPF	[U-15N; U-13C]	1.5 mM
3	phosphate		50 mM
4	NaCl		100 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	ERCC-1	[U-15N]	1 mM
8	XPF	[U-15N]	1 mM
9	phosphate		50 mM
10	NaCl		100 mM
11	D2O		8 %
12	H2O		92 %

3 (h)cCH NOESY HSQC

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	ERCC-1	[U-15N; U-13C]	1.5 mM
2	XPF	[U-15N; U-13C]	1.5 mM
3	phosphate		50 mM
4	NaCl		100 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	ERCC-1	[U-15N]	1 mM
8	XPF	[U-15N]	1 mM
9	phosphate		50 mM
10	NaCl		100 mM
11	D2O		8 %
12	H2O		92 %

4 (h)nCH NOESY HSQC

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	ERCC-1	[U-15N; U-13C]	1.5 mM
2	XPF	[U-15N; U-13C]	1.5 mM
3	phosphate		50 mM
4	NaCl		100 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	ERCC-1	[U-15N]	1 mM
8	XPF	[U-15N]	1 mM
9	phosphate		50 mM
10	NaCl		100 mM
11	D2O		8 %
12	H2O		92 %

5 2D NOESY

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	ERCC-1	[U-15N; U-13C]	1.5 mM
2	XPF	[U-15N; U-13C]	1.5 mM
3	phosphate		50 mM
4	NaCl		100 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	ERCC-1	[U-15N]	1 mM
8	XPF	[U-15N]	1 mM
9	phosphate		50 mM
10	NaCl		100 mM
11	D2O		8 %
12	H2O		92 %

6 2D NOESY 15N filtered

Instrument

Model Bruker AVANCE (900 MHz)

Sample #1

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	ERCC-1	[U-15N; U-13C]	1.5 mM
2	XPF	[U-15N; U-13C]	1.5 mM
3	phosphate		50 mM
4	NaCl		100 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Pressure 1 atm, Temperature 295.5 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	ERCC-1	[U-15N]	1 mM
8	XPF	[U-15N]	1 mM
9	phosphate		50 mM
10	NaCl		100 mM
11	D2O		8 %
12	H2O		92 %

nullKeywords helix-hairpin-helix

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Found 1 Document (3.015 seconds)

BMRB: 6551

Sample

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. ERCC-1, : 1 : 4 : 14 entities Detail

Related entities 2. XPF, : 1 : 2 : 2 : 13 entities Detail

Interaction partners 1. ERCC-1, : 21 : 1 interactors Detail

Interaction partners 2. XPF, : 24 : 1 interactors Detail

Experiments performed 6 experiments Detail

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