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Found 1 Document (1.674 seconds)

BMRB: 6553

1YZA

Specific non-native hydrophobic interactions in a hidden folding intermediate: implication for protein folding

Authors

Feng, H., Takei, T., Lipsitz, R., Tjandra, N., Bai, Y.

Assembly

Redesigned apo-cytochrome b562

Entity

1. Redesigned apo-cytochrome b562 (polymer, Thiol state: not present), 106 monomers, 11430.46 Da Detail

ADLEDNDETG NDNGKGGEKA DNAAQVKDAL TKMRAAALDA QKATPPKLED KSPDSPEMKD FRHGFDILVG QIDDALKLAN EGKVKEAQAA AEQLKTTIRA YNQKYG

Formula weight

11430.46 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry simulated annealing molecular dynamics matrix relaxation torsion angle dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.4 %, Completeness (bb): 85.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.4 % (1017 of 1177)	92.7 % (573 of 618)	74.4 % (331 of 445)	99.1 % (113 of 114)
Backbone	85.4 % (536 of 628)	99.5 % (215 of 216)	71.0 % (220 of 310)	99.0 % (101 of 102)
Sidechain	89.5 % (579 of 647)	89.1 % (358 of 402)	89.7 % (209 of 233)	100.0 % (12 of 12)
Aromatic	25.0 % (10 of 40)	50.0 % (10 of 20)	0.0 % (0 of 20)
Methyl	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)

1. redesigned apo-cytochrome b562

ADLEDNDETG NDNGKGGEKA DNAAQVKDAL TKMRAAALDA QKATPPKLED KSPDSPEMKD FRHGFDILVG QIDDALKLAN EGKVKEAQAA AEQLKTTIRA YNQKYG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹⁵N	na	na	null	null
¹³C	na	na	null	null
¹H	na	na	null	null

Referencing offset: 2.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹⁵N	na	na	null	null
¹³C	na	na	null	null
¹H	na	na	null	null

Referencing offset: 2.3 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹⁵N	na	na	null	null
¹³C	na	na	null	null
¹H	na	na	null	null

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1YZA, Strand ID: A Detail

Coupling constant

76 J values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 4.84 Detail

Release date

2005-08-29

Citation

Specific non-native hydrophobic interactions in a hidden folding intermediate: implications for protein folding
Feng, H., Takei, J., Lipsitz, R., Tjandra, N., Bai, Y.
Biochemistry (2003), 42, 12461-12465, PubMed 14580191 , DOI 10.1021/bi035561s , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 6560 released on 2005-08-29
Title The NMR solution structure of a redesigned apocytochrome b562:Rd-apocyt b562

Related entities 1. Redesigned apo-cytochrome b562, : 1 : 93 : 13 entities Detail

Interaction partners 1. Redesigned apo-cytochrome b562, : 16 interactors Detail

More details for 16 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 2D HSQC

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

2 3D 15N-separated NOESY

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

3 3D 13C-separated NOESY

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

4 HNHA

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

5 3D HNCACB

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

6 3D CBCACONH

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

7 D HAHBCONH

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

8 3D HNCO

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

9 2D NOESY

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

10 2D residual dipolar expts

Instrument

Model Bruker DRX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Concentration
1	redesigned apo-cytochrome b562	0.0 ~ 0.0 mM
2	sodium acetate	0.0 ~ 0.0 mM
3	H2O	92.5 %
4	D2O	7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 4.84

#	Name	Isotope labeling	Type	Concentration
5	redesigned apo-cytochrome b562			0.0 ~ 0.0 mM
6	D2O			99 %

nullKeywords Berkeley Structural Genomics Center, BSGC, Hidden folding Intermediate, hydrophobic-interactions, non-native, protein folding, Protein Structure Initiative, PSI, Rd-apocyt b562, Structural Genomics

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Found 1 Document (1.674 seconds)

BMRB: 6553

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Redesigned apo-cytochrome b562, : 1 : 93 : 13 entities Detail

Interaction partners 1. Redesigned apo-cytochrome b562, : 16 interactors Detail

Experiments performed 10 experiments Detail

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