BMRB: 6554

Experimental evidence for a reversible alfa to beta transition of Abeta-(1-42) in aqueous media: a step by step conformational analysis pinpoints the location of the beta conformation seeding

Authors

Tomaselli, S., Esposito, V., Vangone, P., van Nuland, N.A.J., Bonvin, A.M.J.J., Guerrini, R., Tancredi, T., Temussi, P.A., Picone, D.

Assembly

Alzheimer's disease amyloid

Entity

1. Alzheimer's disease amyloid (polymer, Thiol state: not present), 42 monomers, 4514.037 Da Detail

DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV IA

Formula weight

4514.037 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 55.0 %, Completeness (bb): 56.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	55.0 % (232 of 422)	80.5 % (190 of 236)	22.6 % (42 of 186)
Backbone	56.2 % (118 of 210)	88.9 % (80 of 90)	31.7 % (38 of 120)
Sidechain	48.0 % (119 of 248)	75.3 % (110 of 146)	8.8 % (9 of 102)
Aromatic	20.0 % (10 of 50)	40.0 % (10 of 25)	0.0 % (0 of 25)
Methyl	51.9 % (27 of 52)	92.3 % (24 of 26)	11.5 % (3 of 26)

1. Alzheimer's disease amyloid

DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV IA

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, Details The protein was pretreated with TFA.

#	Name	Isotope labeling	Type	Concentration
1	Alzheimer's disease amyloid			2 mM
2	hexafluoroisopropanol-d2			30 %
3	H2O			70 %

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Protein Blocks Logo

Calculated from 30 models in PDB: 1Z0Q, Strand ID: A Detail

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Release date

2005-03-30

Citation

The alpha-to-beta conformational transition of Alzheimer's Abeta-(1-42) peptide in aqueous media is reversible: a step by step conformational analysis suggests the location of beta conformation seeding
Tomaselli, S., Esposito, V., Vangone, P., van Nuland, N.A.J., Bonvin, A.M.J.J., Guerrini, R., Tancredi, T., Temussi, P.A., Picone, D.
Chembiochem. (2006), 7, 257-267, PubMed 16444756 , DOI 10.1002/cbic.200500223 , Abstract

Related entities 1. Alzheimer's disease amyloid, : 1 : 44 : 3 : 54 : 18 entities Detail

Interaction partners 1. Alzheimer's disease amyloid, : 96 interactors Detail

Experiments performed 3 experiments Detail