BMRBj - BMREntryMaster

Found 1 Document (1.639 seconds)

BMRB: 6555

1YWS

Solution structure of YBL071w-A from Saccharomyces cerevisiae

Authors

Lukin, J.A., Guido, V., Arrowsmith, C.H.

Assembly

protein YBL071w-A

Entity

1. YBL071w-A (polymer, Thiol state: all other bound), 82 monomers, 9356.386 Da Detail

MSTYDEIEIE DMTFEPENQM FTYPCPCGDR FQIYLDDMFE GEKVAVCPSC SLMIDVVFDK EDLAEYYEEA GIHPPEPIAA AA

2. ZN (non-polymer), 65.409 Da

Total weight

9421.795 Da

Max. entity weight

9356.386 Da

Entity Connection

metal coordination 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	2:ZN1:ZN	1:CYS25:SG
2	metal coordination	sing	2:ZN1:ZN	1:CYS27:SG
3	metal coordination	sing	2:ZN1:ZN	1:CYS47:SG
4	metal coordination	sing	2:ZN1:ZN	1:CYS50:SG

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.3 %, Completeness (bb): 99.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.3 % (779 of 935)	79.9 % (386 of 483)	84.2 % (315 of 374)	100.0 % (78 of 78)
Backbone	99.2 % (474 of 478)	100.0 % (160 of 160)	98.4 % (239 of 243)	100.0 % (75 of 75)
Sidechain	71.6 % (384 of 536)	70.0 % (226 of 323)	73.8 % (155 of 210)	100.0 % (3 of 3)
Aromatic	0.0 % (0 of 94)	0.0 % (0 of 47)	0.0 % (0 of 47)
Methyl	95.8 % (69 of 72)	94.4 % (34 of 36)	97.2 % (35 of 36)

1. YBL071w-A

MSTYDEIEIE DMTFEPENQM FTYPCPCGDR FQIYLDDMFE GEKVAVCPSC SLMIDVVFDK EDLAEYYEEA GIHPPEPIAA AA

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	YBL071w-A	[U-15N; U-13C]	1 mM
2	NaCl		450 mM
3	benzamidine		1 mM
4	NaN3		0.01 %
5	MOPS		10 mM
6	ZnSO4		0.01 mM
7	D2O		10 %
8	H2O		90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1YWS, Strand ID: A Detail

Release date

2006-04-05

Citation

Biochemical and structural characterization of a novel family of cystathionine beta-synthase domain proteins fused to a Zn ribbon-like domain
Proudfoot, M., Sanders, S.A., Singer, A., Zhang, R., Brown, G., Binkowski, A., Xu, L., Lukin, J.A., Murzin, A.G., Joachimiak, A., Arrowsmith, C.H., Edwards, A.M., Savchenko, A.V., Yakunin, A.F.
J. Mol. Biol. (2008), 375, 301-315, PubMed 18021800 , DOI 10.1016/j.jmb.2007.10.060 , Abstract

Related entities 1. YBL071w-A, : 1 : 4 : 2 : 18 entities Detail

Interaction partners 1. YBL071w-A, : 25 interactors Detail

More details for 25 interactors identified by 3 citations

Experiments performed 2 experiments Detail

#	Name	Isotope labeling	Concentration
9	YBL071w-A	[U-15N; U-13C]	1 mM
10	NaCl		450 mM
11	benzamidine		1 mM
12	NaN3		0.01 %
13	MOPS		10 mM
14	ZnSO4		0.01 mM
15	D2O		99.9 %

#	Name	Isotope labeling	Concentration
9	YBL071w-A	[U-15N; U-13C]	1 mM
10	NaCl		450 mM
11	benzamidine		1 mM
12	NaN3		0.01 %
13	MOPS		10 mM
14	ZnSO4		0.01 mM
15	D2O		99.9 %

nullKeywords Zinc finger, Structural Genomics, PSI, Protein Structure Initiative, Northeast Structural Genomics Consortium, NESG, Ontario Centre for Structural Proteomics

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Found 1 Document (1.639 seconds)

BMRB: 6555

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. YBL071w-A, : 1 : 4 : 2 : 18 entities Detail

Interaction partners 1. YBL071w-A, : 25 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2