BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	54.5 % (255 of 468)	84.1 % (207 of 246)	2.8 % (5 of 179)	100.0 % (43 of 43)
Backbone	52.5 % (127 of 242)	98.8 % (82 of 83)	5.0 % (6 of 120)	100.0 % (39 of 39)
Sidechain	49.6 % (131 of 264)	76.7 % (125 of 163)	2.1 % (2 of 97)	100.0 % (4 of 4)
Aromatic	42.3 % (22 of 52)	76.9 % (20 of 26)	0.0 % (0 of 24)	100.0 % (2 of 2)
Methyl	44.4 % (8 of 18)	88.9 % (8 of 9)	0.0 % (0 of 9)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
14	Formin-binding protein 3	[U-15N]	1.8 mM
15	phosphate buffer		10 mM
16	Nacl		100 mM
17	DTT		0.1 mM
18	EDTA		0.1 mM
19	H2O		90 %
20	D2O		10 %

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 59.7 %, Completeness (bb): 53.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	59.7 % (559 of 936)	76.0 % (374 of 492)	38.0 % (136 of 358)	57.0 % (49 of 86)
Backbone	53.9 % (261 of 484)	77.7 % (129 of 166)	36.3 % (87 of 240)	57.7 % (45 of 78)
Sidechain	64.2 % (339 of 528)	75.2 % (245 of 326)	46.4 % (90 of 194)	50.0 % (4 of 8)
Aromatic	57.7 % (60 of 104)	76.9 % (40 of 52)	37.5 % (18 of 48)	50.0 % (2 of 4)
Methyl	69.4 % (25 of 36)	88.9 % (16 of 18)	50.0 % (9 of 18)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		10 mM
3	Nacl		100 mM
4	DTT		0.1 mM
5	EDTA		0.1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
9	phosphate buffer		10 mM
10	Nacl		100 mM
11	DTT		0.1 mM
12	EDTA		0.1 mM
13	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹³C	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹³C	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹³C	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1YWJ, Strand ID: A Detail

Release date

2005-08-21

Citation

Structural basis for APPTPPPLPP peptide recognition by the FBP11WW1 domain
Pires, J.R., Parthier, C., Aido-Machado, R., Wiedemann, U., Otte, L., Boehm, G., Rudolph, R., Oschkinat, H.
J. Mol. Biol. (2005), 348, 399-408, PubMed 15811376 , DOI 10.1016/j.jmb.2005.02.056 , Abstract

Related entities 1. Formin-binding protein 3, : 1 : 1 : 103 entities Detail

Experiments performed 5 experiments Detail

1 triple-resonance

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		10 mM
3	Nacl		100 mM
4	DTT		0.1 mM
5	EDTA		0.1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
9	phosphate buffer		10 mM
10	Nacl		100 mM
11	DTT		0.1 mM
12	EDTA		0.1 mM
13	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
14	Formin-binding protein 3	[U-15N]	1.8 mM
15	phosphate buffer		10 mM
16	Nacl		100 mM
17	DTT		0.1 mM
18	EDTA		0.1 mM
19	H2O		90 %
20	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
21	Formin-binding protein 3	1.8 mM
22	phosphate buffer	10 mM
23	Nacl	100 mM
24	DTT	0.1 mM
25	EDTA	0.1 mM
26	H2O	90 %
27	D2O	10 %

2 3D 13C-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		10 mM
3	Nacl		100 mM
4	DTT		0.1 mM
5	EDTA		0.1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
9	phosphate buffer		10 mM
10	Nacl		100 mM
11	DTT		0.1 mM
12	EDTA		0.1 mM
13	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
14	Formin-binding protein 3	[U-15N]	1.8 mM
15	phosphate buffer		10 mM
16	Nacl		100 mM
17	DTT		0.1 mM
18	EDTA		0.1 mM
19	H2O		90 %
20	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
21	Formin-binding protein 3	1.8 mM
22	phosphate buffer	10 mM
23	Nacl	100 mM
24	DTT	0.1 mM
25	EDTA	0.1 mM
26	H2O	90 %
27	D2O	10 %

3 3D 15N-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		10 mM
3	Nacl		100 mM
4	DTT		0.1 mM
5	EDTA		0.1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
9	phosphate buffer		10 mM
10	Nacl		100 mM
11	DTT		0.1 mM
12	EDTA		0.1 mM
13	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
14	Formin-binding protein 3	[U-15N]	1.8 mM
15	phosphate buffer		10 mM
16	Nacl		100 mM
17	DTT		0.1 mM
18	EDTA		0.1 mM
19	H2O		90 %
20	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
21	Formin-binding protein 3	1.8 mM
22	phosphate buffer	10 mM
23	Nacl	100 mM
24	DTT	0.1 mM
25	EDTA	0.1 mM
26	H2O	90 %
27	D2O	10 %

4 2D NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		10 mM
3	Nacl		100 mM
4	DTT		0.1 mM
5	EDTA		0.1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
9	phosphate buffer		10 mM
10	Nacl		100 mM
11	DTT		0.1 mM
12	EDTA		0.1 mM
13	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
14	Formin-binding protein 3	[U-15N]	1.8 mM
15	phosphate buffer		10 mM
16	Nacl		100 mM
17	DTT		0.1 mM
18	EDTA		0.1 mM
19	H2O		90 %
20	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
21	Formin-binding protein 3	1.8 mM
22	phosphate buffer	10 mM
23	Nacl	100 mM
24	DTT	0.1 mM
25	EDTA	0.1 mM
26	H2O	90 %
27	D2O	10 %

5 2D TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	phosphate buffer		10 mM
3	Nacl		100 mM
4	DTT		0.1 mM
5	EDTA		0.1 mM
6	H2O		90 %
7	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
9	phosphate buffer		10 mM
10	Nacl		100 mM
11	DTT		0.1 mM
12	EDTA		0.1 mM
13	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
14	Formin-binding protein 3	[U-15N]	1.8 mM
15	phosphate buffer		10 mM
16	Nacl		100 mM
17	DTT		0.1 mM
18	EDTA		0.1 mM
19	H2O		90 %
20	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
21	Formin-binding protein 3	1.8 mM
22	phosphate buffer	10 mM
23	Nacl	100 mM
24	DTT	0.1 mM
25	EDTA	0.1 mM
26	H2O	90 %
27	D2O	10 %

nullKeywords WW domain, Class II, Proline-rich peptides, protein-protein interactions

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BMRB: 6558

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Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Formin-binding protein 3, : 1 : 1 : 103 entities Detail

Experiments performed 5 experiments Detail

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Sample #4

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Sample #4

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