Structure of the FBP11WW1 domain complexed to the peptide APPTPPPLPP
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 40.7 % (237 of 582) | 61.5 % (190 of 309) | 2.2 % (5 of 227) | 91.3 % (42 of 46) |
Backbone | 41.3 % (119 of 288) | 79.2 % (76 of 96) | 3.3 % (5 of 150) | 90.5 % (38 of 42) |
Sidechain | 34.8 % (119 of 342) | 53.5 % (114 of 213) | 0.8 % (1 of 125) | 100.0 % (4 of 4) |
Aromatic | 42.3 % (22 of 52) | 76.9 % (20 of 26) | 0.0 % (0 of 24) | 100.0 % (2 of 2) |
Methyl | 30.8 % (8 of 26) | 61.5 % (8 of 13) | 0.0 % (0 of 13) |
1. Formin-binding protein 3
GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D2. Formin
APPTPPPLPPPressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | Formin-binding protein 3 | [U-15N] | 1.8 mM | |
17 | Formin | 3.6 mM | ||
18 | phosphate buffer | 10 mM | ||
19 | Nacl | 100 mM | ||
20 | DTT | 0.1 mM | ||
21 | EDTA | 0.1 mM | ||
22 | H2O | 90 % | ||
23 | D2O | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 26.1 % (304 of 1164) | 40.0 % (247 of 618) | 2.9 % (13 of 454) | 47.8 % (44 of 92) |
Backbone | 24.7 % (142 of 576) | 46.4 % (89 of 192) | 4.3 % (13 of 300) | 47.6 % (40 of 84) |
Sidechain | 24.3 % (166 of 684) | 37.1 % (158 of 426) | 1.6 % (4 of 250) | 50.0 % (4 of 8) |
Aromatic | 21.2 % (22 of 104) | 38.5 % (20 of 52) | 0.0 % (0 of 48) | 50.0 % (2 of 4) |
Methyl | 28.8 % (15 of 52) | 53.8 % (14 of 26) | 3.8 % (1 of 26) |
1. Formin-binding protein 3
GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D2. Formin
APPTPPPLPPPressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | Formin-binding protein 3 | [U-15N] | 1.8 mM | |
17 | Formin | 3.6 mM | ||
18 | phosphate buffer | 10 mM | ||
19 | Nacl | 100 mM | ||
20 | DTT | 0.1 mM | ||
21 | EDTA | 0.1 mM | ||
22 | H2O | 90 % | ||
23 | D2O | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 33.1 % (578 of 1746) | 42.5 % (394 of 927) | 20.1 % (137 of 681) | 34.1 % (47 of 138) |
Backbone | 30.3 % (262 of 864) | 45.1 % (130 of 288) | 19.8 % (89 of 450) | 34.1 % (43 of 126) |
Sidechain | 34.8 % (357 of 1026) | 41.3 % (264 of 639) | 23.7 % (89 of 375) | 33.3 % (4 of 12) |
Aromatic | 37.2 % (58 of 156) | 48.7 % (38 of 78) | 25.0 % (18 of 72) | 33.3 % (2 of 6) |
Methyl | 39.7 % (31 of 78) | 56.4 % (22 of 39) | 23.1 % (9 of 39) |
1. Formin-binding protein 3
GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D2. Formin
APPTPPPLPPPressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Formin-binding protein 3 | [U-15N; U-13C] | 1.8 mM | |
2 | Formin | 3.6 mM | ||
3 | phosphate buffer | 10 mM | ||
4 | Nacl | 100 mM | ||
5 | DTT | 0.1 mM | ||
6 | EDTA | 0.1 mM | ||
7 | H2O | 90 % | ||
8 | D2O | 10 % |
Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | Formin-binding protein 3 | [U-15N; U-13C] | 1.8 mM | |
10 | Formin | 3.6 mM | ||
11 | phosphate buffer | 10 mM | ||
12 | Nacl | 100 mM | ||
13 | DTT | 0.1 mM | ||
14 | EDTA | 0.1 mM | ||
15 | D2O | 100 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 27.5 % (641 of 2328) | 36.0 % (445 of 1236) | 16.2 % (147 of 908) | 26.6 % (49 of 184) |
Backbone | 24.9 % (287 of 1152) | 37.2 % (143 of 384) | 16.5 % (99 of 600) | 26.8 % (45 of 168) |
Sidechain | 29.1 % (398 of 1368) | 35.4 % (302 of 852) | 18.4 % (92 of 500) | 25.0 % (4 of 16) |
Aromatic | 27.9 % (58 of 208) | 36.5 % (38 of 104) | 18.8 % (18 of 96) | 25.0 % (2 of 8) |
Methyl | 33.7 % (35 of 104) | 50.0 % (26 of 52) | 17.3 % (9 of 52) |
1. Formin-binding protein 3
GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D2. Formin
APPTPPPLPPPressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Formin-binding protein 3 | [U-15N; U-13C] | 1.8 mM | |
2 | Formin | 3.6 mM | ||
3 | phosphate buffer | 10 mM | ||
4 | Nacl | 100 mM | ||
5 | DTT | 0.1 mM | ||
6 | EDTA | 0.1 mM | ||
7 | H2O | 90 % | ||
8 | D2O | 10 % |
Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | Formin-binding protein 3 | [U-15N; U-13C] | 1.8 mM | |
10 | Formin | 3.6 mM | ||
11 | phosphate buffer | 10 mM | ||
12 | Nacl | 100 mM | ||
13 | DTT | 0.1 mM | ||
14 | EDTA | 0.1 mM | ||
15 | D2O | 100 % |