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Structure of the FBP11WW1 domain complexed to the peptide APPTPPPLPP
Authors
Pires, J.R., Parthier, C., Aido-Machado, R., Wiedemann, U., Otte, L., Boehm, G., Rudolph, R., Oschkinat, H.
Assembly
Formin-binding protein 3/Formin complex
Entity
1. Formin-binding protein 3 (polymer, Thiol state: not present), 41 monomers, 4739.026 Da Detail

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D


2. Formin (polymer, Thiol state: not present), 10 monomers, 983.1575 Da Detail

APPTPPPLPP


Total weight
5722.1836 Da
Max. entity weight
4739.026 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
Automated assignment of NOEs and simulated anneling with torsion angle dinamics
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 80.4 %, Completeness: 40.7 %, Completeness (bb): 41.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All40.7 % (237 of 582)61.5 % (190 of 309) 2.2 % (5 of 227)91.3 % (42 of 46)
Backbone41.3 % (119 of 288)79.2 % (76 of 96) 3.3 % (5 of 150)90.5 % (38 of 42)
Sidechain34.8 % (119 of 342)53.5 % (114 of 213) 0.8 % (1 of 125)100.0 % (4 of 4)
Aromatic42.3 % (22 of 52)76.9 % (20 of 26) 0.0 % (0 of 24)100.0 % (2 of 2)
Methyl30.8 % (8 of 26)61.5 % (8 of 13) 0.0 % (0 of 13)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

2. Formin

APPTPPPLPP

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0


#NameIsotope labelingTypeConcentration
16Formin-binding protein 3[U-15N]1.8 mM
17Formin3.6 mM
18phosphate buffer10 mM
19Nacl100 mM
20DTT0.1 mM
21EDTA0.1 mM
22H2O90 %
23D2O10 %

Chem. Shift Complete2
Sequence coverage: 25.5 %, Completeness: 26.1 %, Completeness (bb): 24.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All26.1 % (304 of 1164)40.0 % (247 of 618) 2.9 % (13 of 454)47.8 % (44 of 92)
Backbone24.7 % (142 of 576)46.4 % (89 of 192) 4.3 % (13 of 300)47.6 % (40 of 84)
Sidechain24.3 % (166 of 684)37.1 % (158 of 426) 1.6 % (4 of 250)50.0 % (4 of 8)
Aromatic21.2 % (22 of 104)38.5 % (20 of 52) 0.0 % (0 of 48)50.0 % (2 of 4)
Methyl28.8 % (15 of 52)53.8 % (14 of 26) 3.8 % (1 of 26)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

2. Formin

APPTPPPLPP

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0


#NameIsotope labelingTypeConcentration
16Formin-binding protein 3[U-15N]1.8 mM
17Formin3.6 mM
18phosphate buffer10 mM
19Nacl100 mM
20DTT0.1 mM
21EDTA0.1 mM
22H2O90 %
23D2O10 %

Chem. Shift Complete3
Sequence coverage: 78.4 %, Completeness: 33.1 %, Completeness (bb): 30.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All33.1 % (578 of 1746)42.5 % (394 of 927)20.1 % (137 of 681)34.1 % (47 of 138)
Backbone30.3 % (262 of 864)45.1 % (130 of 288)19.8 % (89 of 450)34.1 % (43 of 126)
Sidechain34.8 % (357 of 1026)41.3 % (264 of 639)23.7 % (89 of 375)33.3 % (4 of 12)
Aromatic37.2 % (58 of 156)48.7 % (38 of 78)25.0 % (18 of 72)33.3 % (2 of 6)
Methyl39.7 % (31 of 78)56.4 % (22 of 39)23.1 % (9 of 39)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

2. Formin

APPTPPPLPP

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0


#NameIsotope labelingTypeConcentration
1Formin-binding protein 3[U-15N; U-13C]1.8 mM
2Formin3.6 mM
3phosphate buffer10 mM
4Nacl100 mM
5DTT0.1 mM
6EDTA0.1 mM
7H2O90 %
8D2O10 %
Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0


#NameIsotope labelingTypeConcentration
9Formin-binding protein 3[U-15N; U-13C]1.8 mM
10Formin3.6 mM
11phosphate buffer10 mM
12Nacl100 mM
13DTT0.1 mM
14EDTA0.1 mM
15D2O100 %

Chem. Shift Complete4
Sequence coverage: 25.5 %, Completeness: 27.5 %, Completeness (bb): 24.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All27.5 % (641 of 2328)36.0 % (445 of 1236)16.2 % (147 of 908)26.6 % (49 of 184)
Backbone24.9 % (287 of 1152)37.2 % (143 of 384)16.5 % (99 of 600)26.8 % (45 of 168)
Sidechain29.1 % (398 of 1368)35.4 % (302 of 852)18.4 % (92 of 500)25.0 % (4 of 16)
Aromatic27.9 % (58 of 208)36.5 % (38 of 104)18.8 % (18 of 96)25.0 % (2 of 8)
Methyl33.7 % (35 of 104)50.0 % (26 of 52)17.3 % (9 of 52)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

2. Formin

APPTPPPLPP

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0


#NameIsotope labelingTypeConcentration
1Formin-binding protein 3[U-15N; U-13C]1.8 mM
2Formin3.6 mM
3phosphate buffer10 mM
4Nacl100 mM
5DTT0.1 mM
6EDTA0.1 mM
7H2O90 %
8D2O10 %
Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0


#NameIsotope labelingTypeConcentration
9Formin-binding protein 3[U-15N; U-13C]1.8 mM
10Formin3.6 mM
11phosphate buffer10 mM
12Nacl100 mM
13DTT0.1 mM
14EDTA0.1 mM
15D2O100 %

Protein Blocks Logo
Calculated from 15 models in PDB: 1YWI, Strand ID: A, B Detail


Release date
2005-08-21
Citation
Structural basis for APPTPPPLPP peptide recognition by the FBP11WW1 domain
Pires, J.R., Parthier, C., Aido-Machado, R., Wiedemann, U., Otte, L., Boehm, G., Rudolph, R., Oschkinat, H.
J. Mol. Biol. (2005), 348, 399-408, PubMed 15811376 , DOI 10.1016/j.jmb.2005.02.056 ,
Related entities 1. Formin-binding protein 3, : 78 entities Detail
Experiments performed 5 experiments Detail
nullKeywords WW domain, Class II, Proline-rich peptides, protein-protein interactions