BMRBj - BMREntryMaster

Found 1 Document (1.012 seconds)

BMRB: 6559

1YWI

Structure of the FBP11WW1 domain complexed to the peptide APPTPPPLPP

Authors

Pires, J.R., Parthier, C., Aido-Machado, R., Wiedemann, U., Otte, L., Boehm, G., Rudolph, R., Oschkinat, H.

Assembly

Formin-binding protein 3/Formin complex

Entity

1. Formin-binding protein 3 (polymer, Thiol state: not present), 41 monomers, 4739.026 Da Detail

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

2. Formin (polymer, Thiol state: not present), 10 monomers, 983.1575 Da Detail

APPTPPPLPP

Total weight

5722.1836 Da

Max. entity weight

4739.026 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Automated assignment of NOEs and simulated anneling with torsion angle dinamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 80.4 %, Completeness: 40.7 %, Completeness (bb): 41.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	40.7 % (237 of 582)	61.5 % (190 of 309)	2.2 % (5 of 227)	91.3 % (42 of 46)
Backbone	41.3 % (119 of 288)	79.2 % (76 of 96)	3.3 % (5 of 150)	90.5 % (38 of 42)
Sidechain	34.8 % (119 of 342)	53.5 % (114 of 213)	0.8 % (1 of 125)	100.0 % (4 of 4)
Aromatic	42.3 % (22 of 52)	76.9 % (20 of 26)	0.0 % (0 of 24)	100.0 % (2 of 2)
Methyl	30.8 % (8 of 26)	61.5 % (8 of 13)	0.0 % (0 of 13)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

2. Formin

APPTPPPLPP

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	Formin-binding protein 3	[U-15N]	1.8 mM
17	Formin		3.6 mM
18	phosphate buffer		10 mM
19	Nacl		100 mM
20	DTT		0.1 mM
21	EDTA		0.1 mM
22	H2O		90 %
23	D2O		10 %

Chem. Shift Complete2

Sequence coverage: 25.5 %, Completeness: 26.1 %, Completeness (bb): 24.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	26.1 % (304 of 1164)	40.0 % (247 of 618)	2.9 % (13 of 454)	47.8 % (44 of 92)
Backbone	24.7 % (142 of 576)	46.4 % (89 of 192)	4.3 % (13 of 300)	47.6 % (40 of 84)
Sidechain	24.3 % (166 of 684)	37.1 % (158 of 426)	1.6 % (4 of 250)	50.0 % (4 of 8)
Aromatic	21.2 % (22 of 104)	38.5 % (20 of 52)	0.0 % (0 of 48)	50.0 % (2 of 4)
Methyl	28.8 % (15 of 52)	53.8 % (14 of 26)	3.8 % (1 of 26)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

2. Formin

APPTPPPLPP

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	Formin-binding protein 3	[U-15N]	1.8 mM
17	Formin		3.6 mM
18	phosphate buffer		10 mM
19	Nacl		100 mM
20	DTT		0.1 mM
21	EDTA		0.1 mM
22	H2O		90 %
23	D2O		10 %

Chem. Shift Complete3

Sequence coverage: 78.4 %, Completeness: 33.1 %, Completeness (bb): 30.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.1 % (578 of 1746)	42.5 % (394 of 927)	20.1 % (137 of 681)	34.1 % (47 of 138)
Backbone	30.3 % (262 of 864)	45.1 % (130 of 288)	19.8 % (89 of 450)	34.1 % (43 of 126)
Sidechain	34.8 % (357 of 1026)	41.3 % (264 of 639)	23.7 % (89 of 375)	33.3 % (4 of 12)
Aromatic	37.2 % (58 of 156)	48.7 % (38 of 78)	25.0 % (18 of 72)	33.3 % (2 of 6)
Methyl	39.7 % (31 of 78)	56.4 % (22 of 39)	23.1 % (9 of 39)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

2. Formin

APPTPPPLPP

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	Formin		3.6 mM
3	phosphate buffer		10 mM
4	Nacl		100 mM
5	DTT		0.1 mM
6	EDTA		0.1 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
10	Formin		3.6 mM
11	phosphate buffer		10 mM
12	Nacl		100 mM
13	DTT		0.1 mM
14	EDTA		0.1 mM
15	D2O		100 %

Chem. Shift Complete4

Sequence coverage: 25.5 %, Completeness: 27.5 %, Completeness (bb): 24.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	27.5 % (641 of 2328)	36.0 % (445 of 1236)	16.2 % (147 of 908)	26.6 % (49 of 184)
Backbone	24.9 % (287 of 1152)	37.2 % (143 of 384)	16.5 % (99 of 600)	26.8 % (45 of 168)
Sidechain	29.1 % (398 of 1368)	35.4 % (302 of 852)	18.4 % (92 of 500)	25.0 % (4 of 16)
Aromatic	27.9 % (58 of 208)	36.5 % (38 of 104)	18.8 % (18 of 96)	25.0 % (2 of 8)
Methyl	33.7 % (35 of 104)	50.0 % (26 of 52)	17.3 % (9 of 52)

1. Formin-binding protein 3

GSRRASVGSA KSMWTEHKSP DGRTYYYNTE TKQSTWEKPD D

2. Formin

APPTPPPLPP

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	Formin		3.6 mM
3	phosphate buffer		10 mM
4	Nacl		100 mM
5	DTT		0.1 mM
6	EDTA		0.1 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
10	Formin		3.6 mM
11	phosphate buffer		10 mM
12	Nacl		100 mM
13	DTT		0.1 mM
14	EDTA		0.1 mM
15	D2O		100 %

Protein Blocks Logo

Calculated from 15 models in PDB: 1YWI, Strand ID: A, B Detail

Release date

2005-08-21

Citation

Structural basis for APPTPPPLPP peptide recognition by the FBP11WW1 domain
Pires, J.R., Parthier, C., Aido-Machado, R., Wiedemann, U., Otte, L., Boehm, G., Rudolph, R., Oschkinat, H.
J. Mol. Biol. (2005), 348, 399-408, PubMed 15811376 , DOI 10.1016/j.jmb.2005.02.056 , Abstract

Related entities 1. Formin-binding protein 3, : 78 entities Detail

Experiments performed 5 experiments Detail

1 triple-resonance

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	Formin		3.6 mM
3	phosphate buffer		10 mM
4	Nacl		100 mM
5	DTT		0.1 mM
6	EDTA		0.1 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
10	Formin		3.6 mM
11	phosphate buffer		10 mM
12	Nacl		100 mM
13	DTT		0.1 mM
14	EDTA		0.1 mM
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	Formin-binding protein 3	[U-15N]	1.8 mM
17	Formin		3.6 mM
18	phosphate buffer		10 mM
19	Nacl		100 mM
20	DTT		0.1 mM
21	EDTA		0.1 mM
22	H2O		90 %
23	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
24	Formin-binding protein 3	1.8 mM
25	Formin	3.6 mM
26	phosphate buffer	10 mM
27	Nacl	100 mM
28	DTT	0.1 mM
29	EDTA	0.1 mM
30	H2O	90 %
31	D2O	10 %

2 3D 13C-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	Formin		3.6 mM
3	phosphate buffer		10 mM
4	Nacl		100 mM
5	DTT		0.1 mM
6	EDTA		0.1 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
10	Formin		3.6 mM
11	phosphate buffer		10 mM
12	Nacl		100 mM
13	DTT		0.1 mM
14	EDTA		0.1 mM
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	Formin-binding protein 3	[U-15N]	1.8 mM
17	Formin		3.6 mM
18	phosphate buffer		10 mM
19	Nacl		100 mM
20	DTT		0.1 mM
21	EDTA		0.1 mM
22	H2O		90 %
23	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
24	Formin-binding protein 3	1.8 mM
25	Formin	3.6 mM
26	phosphate buffer	10 mM
27	Nacl	100 mM
28	DTT	0.1 mM
29	EDTA	0.1 mM
30	H2O	90 %
31	D2O	10 %

3 3D 15N-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	Formin		3.6 mM
3	phosphate buffer		10 mM
4	Nacl		100 mM
5	DTT		0.1 mM
6	EDTA		0.1 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
10	Formin		3.6 mM
11	phosphate buffer		10 mM
12	Nacl		100 mM
13	DTT		0.1 mM
14	EDTA		0.1 mM
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	Formin-binding protein 3	[U-15N]	1.8 mM
17	Formin		3.6 mM
18	phosphate buffer		10 mM
19	Nacl		100 mM
20	DTT		0.1 mM
21	EDTA		0.1 mM
22	H2O		90 %
23	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
24	Formin-binding protein 3	1.8 mM
25	Formin	3.6 mM
26	phosphate buffer	10 mM
27	Nacl	100 mM
28	DTT	0.1 mM
29	EDTA	0.1 mM
30	H2O	90 %
31	D2O	10 %

4 2D NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	Formin		3.6 mM
3	phosphate buffer		10 mM
4	Nacl		100 mM
5	DTT		0.1 mM
6	EDTA		0.1 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
10	Formin		3.6 mM
11	phosphate buffer		10 mM
12	Nacl		100 mM
13	DTT		0.1 mM
14	EDTA		0.1 mM
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	Formin-binding protein 3	[U-15N]	1.8 mM
17	Formin		3.6 mM
18	phosphate buffer		10 mM
19	Nacl		100 mM
20	DTT		0.1 mM
21	EDTA		0.1 mM
22	H2O		90 %
23	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
24	Formin-binding protein 3	1.8 mM
25	Formin	3.6 mM
26	phosphate buffer	10 mM
27	Nacl	100 mM
28	DTT	0.1 mM
29	EDTA	0.1 mM
30	H2O	90 %
31	D2O	10 %

5 2D TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
2	Formin		3.6 mM
3	phosphate buffer		10 mM
4	Nacl		100 mM
5	DTT		0.1 mM
6	EDTA		0.1 mM
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
9	Formin-binding protein 3	[U-15N; U-13C]	1.8 mM
10	Formin		3.6 mM
11	phosphate buffer		10 mM
12	Nacl		100 mM
13	DTT		0.1 mM
14	EDTA		0.1 mM
15	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	Formin-binding protein 3	[U-15N]	1.8 mM
17	Formin		3.6 mM
18	phosphate buffer		10 mM
19	Nacl		100 mM
20	DTT		0.1 mM
21	EDTA		0.1 mM
22	H2O		90 %
23	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Concentration
24	Formin-binding protein 3	1.8 mM
25	Formin	3.6 mM
26	phosphate buffer	10 mM
27	Nacl	100 mM
28	DTT	0.1 mM
29	EDTA	0.1 mM
30	H2O	90 %
31	D2O	10 %

nullKeywords WW domain, Class II, Proline-rich peptides, protein-protein interactions

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Found 1 Document (1.012 seconds)

BMRB: 6559

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Related entities 1. Formin-binding protein 3, : 78 entities Detail

Experiments performed 5 experiments Detail

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