BMRB: 6564

Chemical Shift Assignment for OSCP-NT (1-120)

Authors

Carbajo, R.J., Kellas, F.A., Runswick, M.J., Walker, J.E., Neuhaus, D.

Assembly

OSCP N-terminal domain

Entity

1. OSCP N-terminal domain (polymer, Thiol state: not present), 120 monomers, 13222.36 Da Detail

FAKLVRPPVQ IYGIEGRYAT ALYSAASKQN KLEQVEKELL RVGQILKEPK MAASLLNPYV KRSVKVKSLS DMTAKEKFSP LTSNLINLLA ENGRLTNTPA VISAFSTMMS VHRGEVPCTV

Formula weight

13222.36 Da

Source organism

Bos taurus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.0 %, Completeness (bb): 84.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.0 % (1272 of 1414)	96.3 % (711 of 738)	79.7 % (441 of 553)	97.6 % (120 of 123)
Backbone	84.4 % (596 of 706)	98.7 % (235 of 238)	70.7 % (251 of 355)	97.3 % (110 of 113)
Sidechain	95.5 % (786 of 823)	95.2 % (476 of 500)	95.8 % (300 of 313)	100.0 % (10 of 10)
Aromatic	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)
Methyl	98.7 % (154 of 156)	97.4 % (76 of 78)	100.0 % (78 of 78)

1. Nt OSCP

FAKLVRPPVQ IYGIEGRYAT ALYSAASKQN KLEQVEKELL RVGQILKEPK MAASLLNPYV KRSVKVKSLS DMTAKEKFSP LTSNLINLLA ENGRLTNTPA VISAFSTMMS VHRGEVPCTV

Show sample condition

Sample

Temperature 300 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	OSCP N-terminal domain	[U-95% 13C; U-90% 15N]		0.5 mM
2	NaCl			0.5 M

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LACS Plot; CA

Referencing offset: -0.13 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 44 models in PDB: 2BO5, Strand ID: A Detail

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Release date

2005-09-21

Citation

Structure of the F(1)-binding Domain of the Stator of Bovine F(1)F(o)-ATPase and How it Binds an alpha-Subunit
Carbajo, R.J., Kellas, F.A., Runswick, M.J., Montgomery, M.G., Walker, J.E., Neuhaus, D.
J. Mol. Biol. (2005), 351, 824-838, PubMed , DOI:

Related entities 1. OSCP N-terminal domain, : 1 : 11 : 1 : 125 entities Detail

Interaction partners 1. OSCP N-terminal domain, : 5 interactors Detail

Experiments performed 9 experiments Detail