BMRBj - BMREntryMaster

Found 1 Document (2.414 seconds)

BMRB: 6570

1ZZP

NMR Assignments of the F-actin binding domain of human Bcr-Abl/c-Abl

Authors

Wiesner, S., Hantschel, O., Mackereth, C.D., Superti-Furga, G., Sattler, M.

Assembly

F-actin binding domain of human Bcr-Abl/c-Abl

Entity

1. F-actin binding domain of human Bcr-Abl/c-Abl (polymer, Thiol state: all free), 130 monomers, 14132.98 Da Detail

GAMASTRVSL RKTRQPPERI ASGAITKGVV LDSTEALCLA ISRNSEQMAS HSAVLEAGKN LYSFCVSYVD SIQQMRNKFA FREAINKLEN NLRELQICPA TAGSGPAATQ DFSKLLSSVK EISDIVQRLE

Formula weight

14132.98 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 87.7 %, Completeness (bb): 95.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.7 % (1287 of 1467)	83.7 % (639 of 763)	90.6 % (512 of 565)	97.8 % (136 of 139)
Backbone	95.1 % (734 of 772)	95.0 % (249 of 262)	94.3 % (362 of 384)	97.6 % (123 of 126)
Sidechain	81.6 % (668 of 819)	77.8 % (390 of 501)	86.9 % (265 of 305)	100.0 % (13 of 13)
Aromatic	51.7 % (31 of 60)	56.7 % (17 of 30)	46.7 % (14 of 30)
Methyl	96.1 % (148 of 154)	96.1 % (74 of 77)	96.1 % (74 of 77)

1. F-actin binding domain of cellular Abelson tyrosine kinase

GAMASTRVSL RKTRQPPERI ASGAITKGVV LDSTEALCLA ISRNSEQMAS HSAVLEAGKN LYSFCVSYVD SIQQMRNKFA FREAINKLEN NLRELQICPA TAGSGPAATQ DFSKLLSSVK EISDIVQRLE

Show sample condition

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.79 ppm	internal	direct	1.0
¹³C	H2O	protons	4.79 ppm	external	indirect	0.2514495
¹⁵N	H2O	protons	4.79 ppm	external	indirect	0.1013291

Referencing offset: 2.65 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.79 ppm	internal	direct	1.0
¹³C	H2O	protons	4.79 ppm	external	indirect	0.2514495
¹⁵N	H2O	protons	4.79 ppm	external	indirect	0.1013291

Referencing offset: 2.65 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.79 ppm	internal	direct	1.0
¹³C	H2O	protons	4.79 ppm	external	indirect	0.2514495
¹⁵N	H2O	protons	4.79 ppm	external	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.79 ppm	internal	direct	1.0
¹³C	H2O	protons	4.79 ppm	external	indirect	0.2514495
¹⁵N	H2O	protons	4.79 ppm	external	indirect	0.1013291

Referencing offset: 2.61 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1ZZP, Strand ID: A Detail

Release date

2005-10-23

Citation

NMR Assignment Reveals an alpha-Helical Fold for the F-Actin Binding Domain of Human Bcr-Abl/c-Abl
Wiesner, S., Hantschel, O., Mackereth, C.D., Superti-Furga, G., Sattler, M.
J. Biomol. NMR (2005), 32, 335-335, PubMed 16211486 , DOI 10.1007/s10858-005-8868-x , Abstract

Related entities 1. F-actin binding domain of human Bcr-Abl/c-Abl, : 1 : 17 entities Detail

Interaction partners 1. F-actin binding domain of human Bcr-Abl/c-Abl, : 61 interactors Detail

More details for 61 interactors identified by 42 citations

Experiments performed 14 experiments Detail

1 HN-HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

2 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

3 CBCANH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

4 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

5 HNHA-J

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

6 HNCO

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

7 CBCACO(N)H

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

8 HN-HSQC-NOESY-(H)C-HMQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

9 HC-HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

10 H(CCO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

11 (H)C(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

12 HC(C)H-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

13 13C-HMQC-NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

14 15N-HSQC-NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DRX (900 MHz)

Sample #1

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 15N]		1.0 mM

Sample #2

Temperature 295 (±0.5) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	F-actin binding domain of cellular Abelson tyrosine kinase	[U-100% 13C; U-100% 15N]		1.0 mM

nullKeywords Bcr-Abl, c-Abl, tyrosine kinase, F-actin binding domain

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Found 1 Document (2.414 seconds)

BMRB: 6570

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Chemical shift reference

Chemical shift reference

Related entities 1. F-actin binding domain of human Bcr-Abl/c-Abl, : 1 : 17 entities Detail

Interaction partners 1. F-actin binding domain of human Bcr-Abl/c-Abl, : 61 interactors Detail

Experiments performed 14 experiments Detail

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