BMRB: 6573

NMR Structure of VPS4A MIT Domain

Authors

Scott, J.A., Gaspar, J., Stuchell, M., Alam, S., Skalicky, J., Sundquist, W.I.

Assembly

vacuolar protein sorting factor 4A

Entity

1. vacuolar protein sorting factor 4A (polymer, Thiol state: all free), 122 monomers, 13972.62 Da Detail

MTTSTLQKAI DLVTKATEED KAKNYEEALR LYQHAVEYFL HAIKYEAHSD KAKESIRAKC VQYLDRAEKL KDYLRSKEKH GKKPVKENQS EGKGSDSDSE GDNPEKKKLQ EQLMGAVVME KP

Formula weight

13972.62 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

automated NOE assignment using CYANA and torsion angle dynamics for structure calculations

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.9 %, Completeness (bb): 83.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.9 % (1294 of 1455)	97.3 % (751 of 772)	75.0 % (416 of 555)	99.2 % (127 of 128)
Backbone	83.7 % (608 of 726)	98.0 % (241 of 246)	69.0 % (249 of 361)	99.2 % (118 of 119)
Sidechain	94.8 % (802 of 846)	97.0 % (510 of 526)	91.0 % (283 of 311)	100.0 % (9 of 9)
Aromatic	78.4 % (58 of 74)	97.3 % (36 of 37)	59.5 % (22 of 37)
Methyl	94.4 % (102 of 108)	100.0 % (54 of 54)	88.9 % (48 of 54)

1. vacuolar protein sorting factor 4A

MTTSTLQKAI DLVTKATEED KAKNYEEALR LYQHAVEYFL HAIKYEAHSD KAKESIRAKC VQYLDRAEKL KDYLRSKEKH GKKPVKENQS EGKGSDSDSE GDNPEKKKLQ EQLMGAVVME KP

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	vacuolar protein sorting factor 4A	[U-13C; U-15N]		0.0 ~ 0.0 mM
2	NaCl			50 mM
3	sodium phosphate			20 mM
4	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.13 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.13 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1YXR, Strand ID: A Detail

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Release date

2005-10-16

Citation

Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A
Scott, A., Gaspar, J., Stuchell-Brereton, M.D., Alam, S.L., Skalicky, J.J., Sundquist, W.I.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 13813-13818, PubMed 16174732 , DOI 10.1073/pnas.0502165102 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. vacuolar protein sorting factor 4A, : 2 : 3 : 33 entities Detail

Interaction partners 1. vacuolar protein sorting factor 4A, : 23 interactors Detail

Experiments performed 2 experiments Detail