BMRBj - BMREntryMaster

Found 1 Document (1.642 seconds)

BMRB: 6574

1YVC

MrR5

Authors

Rossi, P., Aramini, J.M., Xiao, R., Ho, C.K., Ma, L.C., Acton, T.B., Montelione, G.T.

Assembly

MrR5

Entity

1. MrR5 (polymer, Thiol state: not present), 70 monomers, 7501.649 Da Detail

MAFGKPAMKN VPVEAGKEYE VTIEDMGKGG DGIARIDGFV VFVPNAEKGS VINVKVTAVK EKFAFAERVL

Formula weight

7501.649 Da

Source organism

Methanococcus maripaludis

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 94.8 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.8 % (760 of 802)	93.7 % (389 of 415)	95.6 % (303 of 317)	97.1 % (68 of 70)
Backbone	97.6 % (404 of 414)	96.6 % (140 of 145)	98.5 % (199 of 202)	97.0 % (65 of 67)
Sidechain	92.7 % (417 of 450)	92.2 % (249 of 270)	93.2 % (165 of 177)	100.0 % (3 of 3)
Aromatic	72.4 % (42 of 58)	72.4 % (21 of 29)	72.4 % (21 of 29)
Methyl	97.6 % (82 of 84)	95.2 % (40 of 42)	100.0 % (42 of 42)

1. MrR5

MAFGKPAMKN VPVEAGKEYE VTIEDMGKGG DGIARIDGFV VFVPNAEKGS VINVKVTAVK EKFAFAERVL

Show sample condition

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	methyl protons	internal	direct
¹³C	DSS	methyl protons	external	indirect
¹⁵N	DSS	methyl protons	external	indirect

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	methyl protons	internal	direct
¹³C	DSS	methyl protons	external	indirect
¹⁵N	DSS	methyl protons	external	indirect

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	methyl protons	internal	direct
¹³C	DSS	methyl protons	external	indirect
¹⁵N	DSS	methyl protons	external	indirect

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	DSS	methyl protons	internal	direct
¹³C	DSS	methyl protons	external	indirect
¹⁵N	DSS	methyl protons	external	indirect

Referencing offset: 0.5 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1YVC, Strand ID: A Detail

Coupling constant

57 J values in 1 lists
Pressure 1 atm, Temperature 293 K, pH 4.5 Detail

Release date

2005-12-08

Citation

No title is available
Rossi, P., Aramini, J.M., Xiao, R., Ho, C.K., Ma, L.C., Acton, T.B., Montelione, G.T.

Related entities 1. MrR5, : 1 : 50 entities Detail

Experiments performed 9 experiments Detail

1 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

2 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

3 HcCH-COSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

4 Hi-Res CH-HSQC(st. Me assign)

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

5 HNHA

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

6 HetNOE

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

7 TR backbone

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

8 H,C_TOCSYs

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

9 NH-HSQC

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
1	MrR5	[U-15N; U-5% 13C]	1.1 mM
2	DTT		10 mM
3	CaCl2		5 mM
4	NaCl		100 mM
5	NH4OAc		20 mM
6	NaN3		0.02 %
7	D2O		5 %
8	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 4.5

#	Name	Isotope labeling	Concentration
9	MrR5	[U-15N; U-13C]	1.2 mM
10	DTT		10 mM
11	CaCl2		5 mM
12	NaCl		100 mM
13	NH4OAc		20 mM
14	NaN3		0.02 %
15	D2O		5 %
16	H2O		95 %

nullKeywords MrR5, NMR Structure, AutoStructure, AutoAssign, Northeast Structural Genomics, AutoQF, PSI, Protein Structure Initiative, Northeast Structural Genomics Consortium, NESG

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Found 1 Document (1.642 seconds)

BMRB: 6574

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. MrR5, : 1 : 50 entities Detail

Experiments performed 9 experiments Detail

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