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BMRB: 6580

2BBU

assignment of mouse socs3 by NMR

Authors

Babon, J.J., Yao, S., deSouza, D.P., Harrison, C.F., Fabri, L.J., Liepinsch, E., Otting, G., Baca, M., Norton, R.S.

Assembly

socs3 bound to a phosphotyrosine peptide

Entity

1. mouse socs3 (polymer, Thiol state: all free), 164 monomers, 17942.06 Da Detail

LKTFSSKSEY QLVVNAVRKL QESGFYWSAV TGGEANLLLS AEPAGTFLIR DSSDQRHFFT LSVKTQSGTK NLRIQCEGGS FSLQSDPRST QPVPRFDCVL KLVHHYMPPP GTPSFSLPPT EPSSEVPEQP PAQALPGSTP KRAYYIYSGG EKIPLVLSRP LSSN

2. gp130 phosphopeptide (polymer, Thiol state: not present), 15 monomers, 1604.564 Da Detail

STASTVEXST VVHSG

Total weight

19546.625 Da

Max. entity weight

17942.06 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.7 %, Completeness: 64.6 %, Completeness (bb): 82.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.6 % (1308 of 2026)	62.4 % (658 of 1054)	63.2 % (504 of 798)	83.9 % (146 of 174)
Backbone	82.2 % (848 of 1032)	81.4 % (285 of 350)	81.0 % (423 of 522)	87.5 % (140 of 160)
Sidechain	50.8 % (589 of 1160)	53.1 % (374 of 704)	47.3 % (209 of 442)	42.9 % (6 of 14)
Aromatic	39.7 % (62 of 156)	67.9 % (53 of 78)	10.4 % (8 of 77)	100.0 % (1 of 1)
Methyl	73.9 % (133 of 180)	82.2 % (74 of 90)	65.6 % (59 of 90)

1. mouse socs3

LKTFSSKSEY QLVVNAVRKL QESGFYWSAV TGGEANLLLS AEPAGTFLIR DSSDQRHFFT LSVKTQSGTK NLRIQCEGGS FSLQSDPRST QPVPRFDCVL KLVHHYMPPP GTPSFSLPPT EPSSEVPEQP PAQALPGSTP KRAYYIYSGG EKIPLVLSRP LSSN

2. gp130 phosphopeptide

STASTVEXST VVHSG

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.49 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2BBU, Strand ID: A, B Detail

Release date

2005-12-15

Citation

Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain
Babon, J.J., Yao, S., deSouza, D.P., Harrison, C.F., Fabri, L.J., Liepinsh, E., Scrofani, S.D., Baca, M., Norton, R.S.
FEBS J. (2005), 272, 6120-6130, PubMed 16302975 , DOI 10.1111/j.1742-4658.2005.05010.x , Abstract

Related entities 1. mouse socs3, : 1 : 5 entities Detail

Related entities 2. gp130 phosphopeptide, : 1 : 1 : 4 entities Detail

Interaction partners 2. gp130 phosphopeptide, : 9 interactors Detail

More details for 9 interactors identified by 5 citations

Experiments performed 12 experiments Detail

1 15N-HSQC

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

2 13C-HSQC

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

3 13C-NOESY-HSQC

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

4 13C-HNCO

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

5 HNCA

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

6 CBCACONH

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

7 HN(CA)CO

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

8 HCCH-TOCSY

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

9 HCCH-COSY

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

10 15N-HSQC-NOESY

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

11 2D 1H NOESY

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

12 1H TOCSY

Instrument

#1 Model na na (500 MHz)

#2 Model na na (600 MHz)

#3 Model na na (800 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	mouse socs3	[U-13C; U-15N]	0.4 ~ 0.5 mM
2	gp130 phosphopeptide	[U-13C; U-15N]	0.4 ~ 0.5 mM
3	water		0.0 ~ 0.0 mM
4	glutamic acid		50 mM
5	arginine		50 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	mouse socs3	[U-13C]	0.4 ~ 0.5 mM
7	gp130 phosphopeptide	[U-13C]	0.4 ~ 0.5 mM
8	water		0.0 ~ 0.0 mM
9	glutamic acid		50 mM
10	arginine		50 mM

Sample #3

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
11	mouse socs3	[U-15N]	0.4 ~ 0.5 mM
12	gp130 phosphopeptide	[U-15N]	0.4 ~ 0.5 mM
13	water		0.0 ~ 0.0 mM
14	glutamic acid		50 mM
15	arginine		50 mM

Sample #4

Temperature 298 (±0.1) K, pH 6.7 (±0.1)

#	Name	Concentration
16	mouse socs3	0.4 ~ 0.5 mM
17	gp130 phosphopeptide	0.4 ~ 0.5 mM
18	water	0.0 ~ 0.0 mM
19	glutamic acid	50 mM
20	arginine	50 mM

nullKeywords cytokine signalling, Kinase, NMR, PEST sequence, SH2 domain, SOCS

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Found 1 Document (3.379 seconds)

BMRB: 6580

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. mouse socs3, : 1 : 5 entities Detail

Related entities 2. gp130 phosphopeptide, : 1 : 1 : 4 entities Detail

Interaction partners 2. gp130 phosphopeptide, : 9 interactors Detail

Experiments performed 12 experiments Detail

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