BMRBj - BMREntryMaster

Found 1 Document (2.034 seconds)

BMRB: 6594

1Z2Q

Elucidation of the high-resolution solution structure of a trypanosomatid FYVE domain

Authors

Mertens, H.D.T., Callaghan, J.M., McConville, M.J., Gooley, P.R.

Assembly

Leishmania major FYVE domain containing protein 1

Entity

1. Leishmania major FYVE domain containing protein 1 (polymer, Thiol state: all other bound), 84 monomers, 9156.316 Da Detail

GPLGSMGEKQ SKGYWQEDED APACNGCGCV FTTTVRRHHC RNCGYVLCGD CSRHRAAIPM RGITEPERVC DACYLALRSS NMAG

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

9287.135 Da

Max. entity weight

9156.316 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS24:SG	2:ZN1:ZN
2	na	sing	1:CYS51:SG	2:ZN1:ZN
3	na	sing	1:CYS27:SG	2:ZN1:ZN
4	na	sing	1:CYS48:SG	2:ZN1:ZN
5	na	sing	1:CYS40:SG	2:ZN1:ZN
6	na	sing	1:CYS73:SG	2:ZN1:ZN
7	na	sing	1:CYS43:SG	2:ZN1:ZN
8	na	sing	1:CYS70:SG	2:ZN1:ZN

Source organism

Leishmania major

Exptl. method

NMR

Refine. method

torsion angle dynamics, simulated annealing.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 89.4 %, Completeness (bb): 91.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.4 % (801 of 896)	88.9 % (415 of 467)	90.4 % (310 of 343)	88.4 % (76 of 86)
Backbone	91.9 % (456 of 496)	90.8 % (158 of 174)	93.8 % (227 of 242)	88.7 % (71 of 80)
Sidechain	87.1 % (413 of 474)	87.7 % (257 of 293)	86.3 % (151 of 175)	83.3 % (5 of 6)
Aromatic	89.7 % (52 of 58)	100.0 % (29 of 29)	78.6 % (22 of 28)	100.0 % (1 of 1)
Methyl	91.9 % (57 of 62)	96.8 % (30 of 31)	87.1 % (27 of 31)

1. Leishmania major FYVE domain containing protein 1

GPLGSMGEKQ SKGYWQEDED APACNGCGCV FTTTVRRHHC RNCGYVLCGD CSRHRAAIPM RGITEPERVC DACYLALRSS NMAG

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Leishmania major FYVE domain containing protein 1	[U-15N; U-13C]	0.8 mM
2	sodium phosphate buffer		50 mM
3	KCl		50 mM
4	DTT		1 mM
5	AEBSF		1 mM
6	sodium azide		0.05 %
7	H2O		90 %
8	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 21 models in PDB: 1Z2Q, Strand ID: A Detail

Release date

2007-03-26

Citation

A high-resolution solution structure of a trypanosomatid FYVE domain
Mertens, H.D.T., Callaghan, J.M., Swarbrick, J.D., McConville, M.J., Gooley, P.R.
Protein Sci. (2007), 16, 2552-2559, PubMed 17905827 , DOI 10.1110/ps.073009807 , Abstract

Related entities 1. Leishmania major FYVE domain containing protein 1, : 1 : 99 entities Detail

Experiments performed 4 experiments Detail

1 3D 13C-separated NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Leishmania major FYVE domain containing protein 1	[U-15N; U-13C]	0.8 mM
2	sodium phosphate buffer		50 mM
3	KCl		50 mM
4	DTT		1 mM
5	AEBSF		1 mM
6	sodium azide		0.05 %
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	Leishmania major FYVE domain containing protein 1	[U-15N; U-13C]	0.8 mM
10	sodium phosphate buffer		50 mM
11	KCl		50 mM
12	DTT		1 mM
13	AEBSF		1 mM
14	sodium azide		0.05 %
15	D2O		99.99 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
16	Leishmania major FYVE domain containing protein 1	[U-10% 13C]	0.8 mM
17	sodium phosphate buffer		50 mM
18	KCl		50 mM
19	DTT		1 mM
20	AEBSF		1 mM
21	sodium azide		0.05 %
22	H2O		90 %
23	D2O		10 %

2 3D 15N-separated NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Leishmania major FYVE domain containing protein 1	[U-15N; U-13C]	0.8 mM
2	sodium phosphate buffer		50 mM
3	KCl		50 mM
4	DTT		1 mM
5	AEBSF		1 mM
6	sodium azide		0.05 %
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	Leishmania major FYVE domain containing protein 1	[U-15N; U-13C]	0.8 mM
10	sodium phosphate buffer		50 mM
11	KCl		50 mM
12	DTT		1 mM
13	AEBSF		1 mM
14	sodium azide		0.05 %
15	D2O		99.99 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
16	Leishmania major FYVE domain containing protein 1	[U-10% 13C]	0.8 mM
17	sodium phosphate buffer		50 mM
18	KCl		50 mM
19	DTT		1 mM
20	AEBSF		1 mM
21	sodium azide		0.05 %
22	H2O		90 %
23	D2O		10 %

3 HNHA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Leishmania major FYVE domain containing protein 1	[U-15N; U-13C]	0.8 mM
2	sodium phosphate buffer		50 mM
3	KCl		50 mM
4	DTT		1 mM
5	AEBSF		1 mM
6	sodium azide		0.05 %
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	Leishmania major FYVE domain containing protein 1	[U-15N; U-13C]	0.8 mM
10	sodium phosphate buffer		50 mM
11	KCl		50 mM
12	DTT		1 mM
13	AEBSF		1 mM
14	sodium azide		0.05 %
15	D2O		99.99 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
16	Leishmania major FYVE domain containing protein 1	[U-10% 13C]	0.8 mM
17	sodium phosphate buffer		50 mM
18	KCl		50 mM
19	DTT		1 mM
20	AEBSF		1 mM
21	sodium azide		0.05 %
22	H2O		90 %
23	D2O		10 %

4 2D 13C-ct-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Leishmania major FYVE domain containing protein 1	[U-15N; U-13C]	0.8 mM
2	sodium phosphate buffer		50 mM
3	KCl		50 mM
4	DTT		1 mM
5	AEBSF		1 mM
6	sodium azide		0.05 %
7	H2O		90 %
8	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	Leishmania major FYVE domain containing protein 1	[U-15N; U-13C]	0.8 mM
10	sodium phosphate buffer		50 mM
11	KCl		50 mM
12	DTT		1 mM
13	AEBSF		1 mM
14	sodium azide		0.05 %
15	D2O		99.99 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
16	Leishmania major FYVE domain containing protein 1	[U-10% 13C]	0.8 mM
17	sodium phosphate buffer		50 mM
18	KCl		50 mM
19	DTT		1 mM
20	AEBSF		1 mM
21	sodium azide		0.05 %
22	H2O		90 %
23	D2O		10 %

nullKeywords FYVE domain, MEMBRANE PROTEIN, Zinc-finger

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Found 1 Document (2.034 seconds)

BMRB: 6594

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Leishmania major FYVE domain containing protein 1, : 1 : 99 entities Detail

Experiments performed 4 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3