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Human ARNT C-terminal PAS domain
Authors
Card, P.C., Erbel, J.A., Gardner, K.H.
Assembly
Aryl hydrocarbon receptor nuclear translocator
Entity
1. Aryl hydrocarbon receptor nuclear translocator (polymer, Thiol state: all free), 119 monomers, 13942.58 Da Detail

GAMDNVCQPT EFISRHNIEG IFTFVDHRCV ATVGYQPQEL LGKNIVEFCH PEDQQLLRDS FQQVVKLKGQ VLSVMFRFRS KNQEWLWMRT SSFTFQNPYS DEIEYIICTN TNVKNSSQE


Formula weight
13942.58 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.2 %, Completeness: 97.4 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All97.4 % (1408 of 1446)97.6 % (737 of 755)96.9 % (538 of 555)97.8 % (133 of 136)
Backbone97.7 % (690 of 706)97.5 % (233 of 239)97.7 % (344 of 352)98.3 % (113 of 115)
Sidechain97.4 % (832 of 854)97.7 % (504 of 516)97.2 % (308 of 317)95.2 % (20 of 21)
Aromatic93.3 % (140 of 150)96.0 % (72 of 75)90.4 % (66 of 73)100.0 % (2 of 2)
Methyl99.1 % (113 of 114)98.2 % (56 of 57)100.0 % (57 of 57)

1. hARNT

GAMDNVCQPT EFISRHNIEG IFTFVDHRCV ATVGYQPQEL LGKNIVEFCH PEDQQLLRDS FQQVVKLKGQ VLSVMFRFRS KNQEWLWMRT SSFTFQNPYS DEIEYIICTN TNVKNSSQE

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1hARNT[U-15N; U-13C]1 mM
2Tris buffer50 mM
3NaCl17 mM
4DTT5 mM
5H2O90 %
6D2O10 %
Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
7hARNT[U-15N]0.478 mM
8Tris buffer50 mM
9NaCl17 mM
10DTT5 mM
11H2O93 %
12D2O7 %

LACS Plot; CA
Referencing offset: -0.18 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.18 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.03 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.26 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1X0O, Strand ID: A Detail


Release date
2005-10-30
Citation
Structural basis of ARNT PAS-B dimerization: use of a common beta-sheet interface for hetero- and homodimerization
Card, P.B., Erbel, P.J., Gardner, K.H.
J. Mol. Biol. (2005), 353, 664-677, PubMed 16181639 , DOI 10.1016/j.jmb.2005.08.043 ,
Related entities 1. Aryl hydrocarbon receptor nuclear translocator, : 1 : 3 : 26 : 248 entities Detail
Interaction partners 1. Aryl hydrocarbon receptor nuclear translocator, : 29 interactors Detail
Experiments performed 10 experiments Detail
nullKeywords PAS, mixed alpha-beta fold, hypoxia, aryl hydrocarbon receptor nuclear translocator, ARNT