BMRB: 6600

Solution Structure of Bacillus subtilis s-BCCP apo-form

Authors

Cui, G., Xia, B.

Assembly

Biotin Carboxyl Carrier Protein

Entity

1. Biotin Carboxyl Carrier Protein (polymer, Thiol state: not present), 72 monomers, 7910.012 Da Detail

TVSIQMAGNL WKVHVKAGDQ IEKGQEVAIL ESMKMEIPIV ADRSGIVKEV KKKEGDFVNE GDVLLELSNS TQ

Formula weight

7910.012 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

simulated annealing molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.4 %, Completeness (bb): 99.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.4 % (831 of 836)	99.5 % (434 of 436)	99.1 % (318 of 321)	100.0 % (79 of 79)
Backbone	99.1 % (426 of 430)	99.3 % (148 of 149)	98.6 % (207 of 210)	100.0 % (71 of 71)
Sidechain	99.8 % (471 of 472)	99.7 % (286 of 287)	100.0 % (177 of 177)	100.0 % (8 of 8)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (12 of 12)	100.0 % (1 of 1)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. Biotin Carboxyl Carrier Protein

TVSIQMAGNL WKVHVKAGDQ IEKGQEVAIL ESMKMEIPIV ADRSGIVKEV KKKEGDFVNE GDVLLELSNS TQ

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	Biotin Carboxyl Carrier Protein	[U-13C; U-15N]		1.2 mM
2	phosphate buffer			50 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.36 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	null	1.0

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LACS Plot; CB

Referencing offset: -0.36 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	null	1.0

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	null	1.0

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LACS Plot; CO

Referencing offset: 0.68 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	null	1.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1Z7T, Strand ID: A Detail

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Release date

2007-01-25

Citation

Identification and solution structures of a single domain biotin/lipoyl attachment protein from Bacillus subtilis
Cui, G., Nan, B., Hu, J., Wang, Y., Jin, C., Xia, B.
J. Biol. Chem. (2006), 281, 20598-20607, PubMed 16699181 , DOI 10.1074/jbc.M602660200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. Biotin Carboxyl Carrier Protein, : 1 : 4 : 230 entities Detail

Experiments performed 2 experiments Detail