BMRB: 6615

Backbone 1H, 13C, and 15N Chemical Shift Assignments for SSD domain of Bacillus subtilis Lon protease

Authors

Wang, I., Lou, Y., Lo, S., Lee, Y.ALAN., Wu, S., Chen, C.

Assembly

BsLonSSD monomer

Entity

1. BsLonSSD monomer (polymer, Thiol state: all free), 124 monomers, 14365.40 Da Detail

MAGYTEIEKL EIVKDHLLPK QIKEHGLKKS NLQLRDQAIL DIIRYYTREA GVRSLERQLA AICRKAAKAI VAEERKRITV TEKNLQDFIG KRIFRYGQAE TEDQVGVVTG LAYTTVLRHH HHHH

Formula weight

14365.4 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 89.6 %, Completeness (bb): 92.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.6 % (1339 of 1495)	88.1 % (689 of 782)	90.7 % (527 of 581)	93.2 % (123 of 132)
Backbone	92.3 % (685 of 742)	90.9 % (231 of 254)	93.2 % (340 of 365)	92.7 % (114 of 123)
Sidechain	87.8 % (764 of 870)	86.7 % (458 of 528)	89.2 % (297 of 333)	100.0 % (9 of 9)
Aromatic	73.9 % (68 of 92)	73.9 % (34 of 46)	73.9 % (34 of 46)
Methyl	96.3 % (156 of 162)	97.5 % (79 of 81)	95.1 % (77 of 81)

1. BsLonSSD

MAGYTEIEKL EIVKDHLLPK QIKEHGLKKS NLQLRDQAIL DIIRYYTREA GVRSLERQLA AICRKAAKAI VAEERKRITV TEKNLQDFIG KRIFRYGQAE TEDQVGVVTG LAYTTVLRHH HHHH

Show sample condition

Sample

Temperature 310 (±0.5) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BsLonSSD	[U-95% 13C; U-95% 15N]		1.5 (±0.2) mM
2	D2O			10 %
3	phosphate buffer			50 mM
4	sodium chloride			100 mM
5	Argnine			50 mM
6	glutamic acid			50 mM
7	DTT			5 mM

Hide sample codition

LACS Plot; CA

Referencing offset: 0.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.21 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.15 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 16 models in PDB: 1X37, Strand ID: A Detail

---

Release date

2005-12-28

Citation

(1)H, (13)C and (15)N resonance assignments of alpha-domain for Bacillus subtilis Lon protease
Wang, I., Lou, Y., Lin, Y., Lo, S., Lee, A., Wu, S., Chen, C.
Biomol. NMR Assign. (2007), 1, 201-203, PubMed 19636865 , DOI 10.1007/s12104-007-9056-6 , Abstract

Related entities 1. BsLonSSD monomer, : 1 : 122 entities Detail

Interaction partners 1. BsLonSSD monomer, : 1 interactors Detail

Experiments performed 6 experiments Detail