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BMRB: 6620

2GOV

1H, 13C, and 15N Chemical Shift Assignments for murine p22HBP

Authors

Dias, J.S., Macedo, A.L., Ferreira, G.C., Jeanty, N., Taketani, S., Goodfellow, B.J., Peterson, F., Volkman, B.F.

Assembly

p22HBP monomer

Entity

1. p22HBP monomer (polymer, Thiol state: not present), 195 monomers, 21725.15 Da Detail

MKQSTHHHHI HNSLFGSVET WPWQVLSTGG KEDVSYEERA CEGGKFATVE VTDKPVDEAL REAMPKIMKY VGGTNDKGVG MGMTVPVSFA VFPNEDGSLQ KKLKVWFRIP NQFQGSPPAP SDESVKIEER EGITVYSTQF GGYAKEADYV AHATQLRTTL EGTPATYQGD VYYCAGYDPP MKPYGRRNEV WLVKA

Formula weight

21725.15 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 69.0 %, Completeness (bb): 74.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.0 % (1550 of 2245)	73.9 % (862 of 1166)	59.8 % (526 of 880)	81.4 % (162 of 199)
Backbone	74.5 % (852 of 1144)	86.4 % (342 of 396)	62.2 % (352 of 566)	86.8 % (158 of 182)
Sidechain	66.4 % (848 of 1277)	67.5 % (520 of 770)	66.1 % (324 of 490)	23.5 % (4 of 17)
Aromatic	56.8 % (126 of 222)	70.3 % (78 of 111)	41.1 % (44 of 107)	100.0 % (4 of 4)
Methyl	83.7 % (149 of 178)	85.4 % (76 of 89)	82.0 % (73 of 89)

1. murine heme binding protein p22HBP

Show sample condition

Sample #1

Temperature 303 (±0.1) K, pH 8.0 (±0.01), Details 50mM phosphate buffer, 100mM KCl

#	Name	Isotope labeling	Concentration
1	murine heme binding protein p22HBP	[U-15N; U-13C]	1 mM
2	phosphate buffer		50 mM
3	KCL		100 mM

Sample #2

Temperature 303 (±0.1) K, pH 8.0 (±0.01), Details 50mM phosphate buffer, 100mM KCl

#	Name	Isotope labeling	Concentration
7	murine heme binding protein p22HBP	[U-15N]	1 mM
8	phosphate buffer		50 mM
9	KCL		100 mM

Sample #3

Temperature 303 (±0.1) K, pH 8.0 (±0.01), Details 50mM phosphate buffer, 100mM KCl

#	Name	Isotope labeling	Concentration
10	murine heme binding protein p22HBP	[U-15N; U-13C; U-2H]	1 mM
11	phosphate buffer		50 mM
12	KCL		100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.52 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.52 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2GOV, Strand ID: A Detail

Release date

2005-10-09

Citation 1

The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP
Dias, J.S., Macedo, A.L., Ferreria, G.C., Peterson, F.C., Volkman, B.F., Goodfellow, B.J.
J. Biol. Chem. (2006), 281, 31553-31561, PubMed 16905545 , DOI 10.1074/jbc.M605988200 , Abstract

Show more 1 citaion

Citation 2

1H, 15N and 13C resonance assignments of the heme-binding protein murine p22HBP
Dias, J.S., Macedo, A.L., Ferreria, G.C., Jeanty, N., Taketani, S., Goodfellow, B.J., Peterson, F.C., Volkman, B.F.
J. Biomol. NMR (2005), 32, 338-338, PubMed 16211492 , DOI 10.1007/s10858-005-0470-8 , Abstract

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9R257 released on 2006-01-24
Title HEBP1_MOUSE Entity Heme-binding protein 1

Related entities 1. p22HBP monomer, : 1 : 3 : 28 entities Detail

Interaction partners 1. p22HBP monomer, : 7 interactors Detail

More details for 7 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 2D 1H15N HSQC