Backbone 1H, 13C, and 15N Resonance Assignments for the 13kD Ras Association Domain 1 (RA1) from Phospholipase C (epsilon)
GEERKCLQTH RVTVHGVPGP EPFTVFTING GTKAKQLLQQ ILTNEQDIKP VTTDYFLMEE KYFISKEKNE CRKQPFQRAI GPEEEIMQIL SSWFPEEGYM GRIVLKTQQE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.6 % (1023 of 1353) | 79.1 % (566 of 716) | 70.0 % (364 of 520) | 79.5 % (93 of 117) |
Backbone | 82.5 % (533 of 646) | 86.4 % (191 of 221) | 78.9 % (254 of 322) | 85.4 % (88 of 103) |
Sidechain | 70.2 % (568 of 809) | 75.8 % (375 of 495) | 62.7 % (188 of 300) | 35.7 % (5 of 14) |
Aromatic | 14.4 % (15 of 104) | 26.9 % (14 of 52) | 0.0 % (0 of 51) | 100.0 % (1 of 1) |
Methyl | 96.2 % (102 of 106) | 96.2 % (51 of 53) | 96.2 % (51 of 53) |
1. ra1
GEERKCLQTH RVTVHGVPGP EPFTVFTING GTKAKQLLQQ ILTNEQDIKP VTTDYFLMEE KYFISKEKNE CRKQPFQRAI GPEEEIMQIL SSWFPEEGYM GRIVLKTQQETemperature 298 K, pH 7.0 (±0.2), Details 15N labelled RA1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ra1 | [U-15N] | protein | 1 mM |
2 | sodium chloride | salt | 250 mM | |
3 | DTT | buffer | 5 mM | |
4 | TRIS | buffer | 25 mM | |
5 | EDTA | buffer | 0.1 mM |
Temperature 298 K, pH 7.0 (±0.2), Details 13C/15N labelled RA1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | ra1 | [U-13C; U-15N] | protein | 1 mM |
7 | sodium chloride | salt | 250 mM | |
8 | DTT | buffer | 5 mM | |
9 | TRIS | buffer | 25 mM | |
10 | EDTA | buffer | 0.1 mM |