BMRB: 6625

1H, 13C and 15N assignments of a New Phosphohistidine Phosphatase Protein PHPT1 from Homo sapiens

Authors

Gong, W., Cui, G., Jin, C., Xia, B.

Assembly

PHPT1

Entity

1. PHPT1 (polymer, Thiol state: all free), 125 monomers, 13832.36 Da Detail

MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY

Formula weight

13832.36 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 84.8 %, Completeness (bb): 92.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.8 % (1195 of 1409)	82.1 % (598 of 728)	87.2 % (482 of 553)	89.8 % (115 of 128)
Backbone	92.2 % (682 of 740)	90.6 % (232 of 256)	94.2 % (343 of 364)	89.2 % (107 of 120)
Sidechain	79.6 % (623 of 783)	77.5 % (366 of 472)	82.2 % (249 of 303)	100.0 % (8 of 8)
Aromatic	48.5 % (65 of 134)	49.3 % (33 of 67)	46.2 % (30 of 65)	100.0 % (2 of 2)
Methyl	95.0 % (114 of 120)	95.0 % (57 of 60)	95.0 % (57 of 60)

1. PHPT1

MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 7.2 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PHPT1	[U-15N; U-13C]		0.8 mM

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LACS Plot; CA

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.16 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 21 models in PDB: 2AI6, Strand ID: A Detail

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Release date

2008-01-27

Citation

(1)H, (13)C, and (15)N resonance assignments of human phosphohistidine phosphatase 1 (PHPT1)
Gong, W., Cui, G., Jin, C., Xia, B.
Biomol. NMR Assign. (2007), 1, 229-231, PubMed 19636872 , DOI 10.1007/s12104-007-9064-6 , Abstract

Related entities 1. PHPT1, : 1 : 4 : 2 : 30 entities Detail

Interaction partners 1. PHPT1, : 6 interactors Detail

Experiments performed 9 experiments Detail