BMRB: 6629

Solution Structure of the type 1 pilus assembly platform FimD(25-139)

Authors

Nishiyama, M., Horst, R., Hermann, T., Vetsch, M., Bettendorff, P., Ignatov, O., Grutter, M., Wuthrich, K., Glockshuber, R., Capitani, G.

Assembly

Outer membrane usher protein FimD

Entity

1. Outer membrane usher protein FimD (polymer, Thiol state: all free), 115 monomers, 12382.86 Da Detail

GQELPPGTYR VDIYLNNGYM ATRDVTFNTG DSEQGIVPCL TRAQLASMGL NTASVAGMNL LADDACVPLT TMVQDATAHL DVGQQRLNLT IPQAFMSNRA RGYIPPELWD PGINA

Formula weight

12382.86 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.6 %, Completeness (bb): 83.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.6 % (1106 of 1263)	94.5 % (613 of 649)	76.4 % (375 of 491)	95.9 % (118 of 123)
Backbone	83.2 % (561 of 674)	97.0 % (225 of 232)	69.3 % (232 of 335)	97.2 % (104 of 107)
Sidechain	93.7 % (650 of 694)	93.0 % (388 of 417)	95.0 % (248 of 261)	87.5 % (14 of 16)
Aromatic	75.0 % (51 of 68)	85.3 % (29 of 34)	63.6 % (21 of 33)	100.0 % (1 of 1)
Methyl	98.6 % (138 of 140)	98.6 % (69 of 70)	98.6 % (69 of 70)

1. E. coli FimD

GQELPPGTYR VDIYLNNGYM ATRDVTFNTG DSEQGIVPCL TRAQLASMGL NTASVAGMNL LADDACVPLT TMVQDATAHL DVGQQRLNLT IPQAFMSNRA RGYIPPELWD PGINA

Show sample condition

Sample

Pressure 1 atm, Temperature 293 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	E. coli FimD	[U-13C; U-15N]		0.0 ~ 0.0 mM
2	H2O			90 %
3	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.71 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.71 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1ZDV, Strand ID: A Detail

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Release date

2005-10-17

Citation

Structural basis of chaperone-subunit complex recognition by the type 1 pilus assembly platform FimD
Nishiyama, M., Horst, R., Hermann, T., Vetsch, M., Bettendorff, P., Ignatov, O., Grutter, M., Wuthrich, K., Glockshuber, R., Capitani, G.
EMBO J. (2005), 24, 2075-2086, PubMed 15920478 , DOI 10.1038/sj.emboj.7600693 , Abstract

Related entities 1. Outer membrane usher protein FimD, : 1 : 4 : 3 : 39 entities Detail

Interaction partners 1. Outer membrane usher protein FimD, : 6 interactors Detail

Experiments performed 2 experiments Detail