BMRB: 6631

Solution structure of a human ubiquitin-like domain in SF3A1

Authors

Lukin, J.A., Dhe-Paganon, S., Guido, V., Arrowsmith, C.H.

Assembly

Splicing factor 3 subunit 1

Entity

1. Splicing factor 3 subunit 1 (polymer, Thiol state: not present), 86 monomers, 9553.040 Da Detail

PVSIKVQVPN MQDKTEWKLN GQVLVFTLPL TDQVSVIKVK IHEATGMPAG KQKLQYEGIF IKDSNSLAYY NMANGAVIHL ALKERG

Formula weight

9553.04 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

100 structures were calculated by CYANA 2.0 using 2667 NOE-derived distance constraints, 121 dihedral angle

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 90.2 %, Completeness (bb): 96.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.2 % (930 of 1031)	88.1 % (473 of 537)	91.5 % (366 of 400)	96.8 % (91 of 94)
Backbone	96.1 % (488 of 508)	95.4 % (166 of 174)	96.4 % (243 of 252)	96.3 % (79 of 82)
Sidechain	86.2 % (520 of 603)	84.6 % (307 of 363)	88.2 % (201 of 228)	100.0 % (12 of 12)
Aromatic	51.6 % (33 of 64)	62.5 % (20 of 32)	38.7 % (12 of 31)	100.0 % (1 of 1)
Methyl	97.3 % (109 of 112)	98.2 % (55 of 56)	96.4 % (54 of 56)

1. Splicing factor 3

PVSIKVQVPN MQDKTEWKLN GQVLVFTLPL TDQVSVIKVK IHEATGMPAG KQKLQYEGIF IKDSNSLAYY NMANGAVIHL ALKERG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	Splicing factor 3	[U-15N; U-13C]		2 mM
2	NaCl			300 mM
3	benzamidine			1 mM
4	DTT			10 mM
5	NaN3			0.01 %
6	Sodium Acetate			10 mM
7	ZnSO4			0.01 mM
8	H2O			90 %
9	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.26 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1ZKH, Strand ID: A Detail

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Release date

2007-11-12

Citation

Solution structure of a human ubiquitin-like domain in SF3A1
Avvakumov, G.V., Xue, S., Newman, E.M., Mackenzie, F., Dhe-Paganon, S.

Related entities 1. Splicing factor 3 subunit 1, : 1 : 16 : 21 entities Detail

Interaction partners 1. Splicing factor 3 subunit 1, : 62 interactors Detail

Experiments performed 2 experiments Detail