BMRBj - BMREntryMaster

Found 1 Document (1.299 seconds)

BMRB: 6633

1ZC5

Structure of the RNA signal essential for translational frameshifting in HIV-1

Authors

Gaudin, C., Mazauric, M.H., Traikia, M., Guittet, E., Yoshizawa, S., Fourmy, D.

Assembly

HIV-1 frameshift RNA signal

Entity

1. HIV-1 frameshift RNA signal (polymer, Thiol state: not present), 41 monomers, 13313.91 Da Detail

GGCGAUCUGG CCUUCCUACA AGGGAAGGCC AGGGAAUUGC C

Formula weight

13313.91 Da

Source organism

Lentivirus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.6 %, Completeness (bb): 99.8 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	98.6 % (642 of 651)	98.3 % (351 of 357)	100.0 % (273 of 273)	85.7 % (18 of 21)
Suger, PO4	99.8 % (450 of 451)	99.6 % (245 of 246)	100.0 % (205 of 205)
Nucleobase	96.0 % (192 of 200)	95.5 % (106 of 111)	100.0 % (68 of 68)	85.7 % (18 of 21)
Aromatic	96.6 % (172 of 178)	96.6 % (86 of 89)	100.0 % (68 of 68)	85.7 % (18 of 21)

1. HIV frameshift RNA signal

GGCGAUCUGG CCUUCCUACA AGGGAAGGCC AGGGAAUUGC C

Show sample condition

Sample #1

Pressure 1 atm, Temperature 283 (±0.1) K, pH 6.4 (±0.1)

#	Name	Concentration
1	HIV frameshift RNA signal	1 mM
2	Na phosphate buffer	10 mM
3	D2O	10 %
4	H2O	90 %

Sample #2

Pressure 1 atm, Temperature 283 (±0.1) K, pH 6.4 (±0.1)

#	Name	Concentration
5	HIV frameshift RNA signal	1 mM
6	Na phosphate buffer	10 mM
7	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 283 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
8	HIV frameshift RNA signal	[U-13C; U-15N]	1 mM
9	Na phosphate buffer		10 mM
10	D2O		100 %

Release date

2005-08-17

Citation

Structure of the RNA signal essential for translational frameshifting in HIV-1
Gaudin, C., Mazauric, M.H., Traikia, M., Guittet, E., Yoshizawa, S., Fourmy, D.
J. Mol. Biol. (2005), 349, 1024-1035, PubMed 15907937 , DOI 10.1016/j.jmb.2005.04.045 , Abstract

Related entities 1. HIV-1 frameshift RNA signal, : 1 : 2 entities Detail

Experiments performed 7 experiments Detail

1 2D NOESY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
1	HIV frameshift RNA signal	1 mM
2	Na phosphate buffer	10 mM
3	D2O	10 %
4	H2O	90 %

Sample #2

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
5	HIV frameshift RNA signal	1 mM
6	Na phosphate buffer	10 mM
7	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
8	HIV frameshift RNA signal	[U-13C; U-15N]	1 mM
9	Na phosphate buffer		10 mM
10	D2O		100 %

2 2D TOCSY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
1	HIV frameshift RNA signal	1 mM
2	Na phosphate buffer	10 mM
3	D2O	10 %
4	H2O	90 %

Sample #2

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
5	HIV frameshift RNA signal	1 mM
6	Na phosphate buffer	10 mM
7	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
8	HIV frameshift RNA signal	[U-13C; U-15N]	1 mM
9	Na phosphate buffer		10 mM
10	D2O		100 %

3 DQF-COSY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
1	HIV frameshift RNA signal	1 mM
2	Na phosphate buffer	10 mM
3	D2O	10 %
4	H2O	90 %

Sample #2

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
5	HIV frameshift RNA signal	1 mM
6	Na phosphate buffer	10 mM
7	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
8	HIV frameshift RNA signal	[U-13C; U-15N]	1 mM
9	Na phosphate buffer		10 mM
10	D2O		100 %

4 HP COSY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
1	HIV frameshift RNA signal	1 mM
2	Na phosphate buffer	10 mM
3	D2O	10 %
4	H2O	90 %

Sample #2

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
5	HIV frameshift RNA signal	1 mM
6	Na phosphate buffer	10 mM
7	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
8	HIV frameshift RNA signal	[U-13C; U-15N]	1 mM
9	Na phosphate buffer		10 mM
10	D2O		100 %

5 3D-HCCH-TOCSY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
1	HIV frameshift RNA signal	1 mM
2	Na phosphate buffer	10 mM
3	D2O	10 %
4	H2O	90 %

Sample #2

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
5	HIV frameshift RNA signal	1 mM
6	Na phosphate buffer	10 mM
7	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
8	HIV frameshift RNA signal	[U-13C; U-15N]	1 mM
9	Na phosphate buffer		10 mM
10	D2O		100 %

6 HSQC

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
1	HIV frameshift RNA signal	1 mM
2	Na phosphate buffer	10 mM
3	D2O	10 %
4	H2O	90 %

Sample #2

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
5	HIV frameshift RNA signal	1 mM
6	Na phosphate buffer	10 mM
7	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
8	HIV frameshift RNA signal	[U-13C; U-15N]	1 mM
9	Na phosphate buffer		10 mM
10	D2O		100 %

7 3D HCP

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Varian INOVA (500 MHz)

Sample #1

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
1	HIV frameshift RNA signal	1 mM
2	Na phosphate buffer	10 mM
3	D2O	10 %
4	H2O	90 %

Sample #2

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Concentration
5	HIV frameshift RNA signal	1 mM
6	Na phosphate buffer	10 mM
7	D2O	100 %

Sample #3

Pressure 1 atm, pH 6.4 (±0.1)

#	Name	Isotope labeling	Concentration
8	HIV frameshift RNA signal	[U-13C; U-15N]	1 mM
9	Na phosphate buffer		10 mM
10	D2O		100 %

nullKeywords RNA bulged helix

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Found 1 Document (1.299 seconds)

BMRB: 6633

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Related entities 1. HIV-1 frameshift RNA signal, : 1 : 2 entities Detail

Experiments performed 7 experiments Detail

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