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BMRB: 6635

2BYF

Backbone 1H, 13C, and 15N Resonance Assignments for the 13kD Ras Association Domain 2 (RA2) from Phospholipase C (epsilon)

Authors

Harris, R., Bunney, T.D., Katan, M., Driscoll, P.C.

Assembly

RA2

Entity

1. RA2 (polymer, Thiol state: all free), 117 monomers, 13146.70 Da Detail

GSSEEESFFV QVHDVSPEQP LTVIKAPRVS TAQDVIQQTL CKAKYSYSIL SNPNPSDYVL LEEVVKDTTN KKTTTPKSSQ RVLLDQECVF QAQSKWKGAG KFILKLKEQV QASREDK

Formula weight

13146.7 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

RESTRAINED SIMULATED ANNEALING, WATER REFINEMENT

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 85.6 %, Completeness (bb): 88.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.6 % (1190 of 1390)	88.3 % (644 of 729)	83.0 % (444 of 535)	81.0 % (102 of 126)
Backbone	88.0 % (607 of 690)	93.5 % (216 of 231)	83.6 % (291 of 348)	90.1 % (100 of 111)
Sidechain	83.9 % (683 of 814)	85.9 % (428 of 498)	84.1 % (253 of 301)	13.3 % (2 of 15)
Aromatic	97.5 % (78 of 80)	97.5 % (39 of 40)	97.4 % (38 of 39)	100.0 % (1 of 1)
Methyl	89.1 % (114 of 128)	95.3 % (61 of 64)	82.8 % (53 of 64)

1. ra2

GSSEEESFFV QVHDVSPEQP LTVIKAPRVS TAQDVIQQTL CKAKYSYSIL SNPNPSDYVL LEEVVKDTTN KKTTTPKSSQ RVLLDQECVF QAQSKWKGAG KFILKLKEQV QASREDK

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2BYF, Strand ID: A Detail

Release date

2005-11-06

Citation

Backbone 1H, 13C, and 15N resonance assignments for the two 13 kD Ras associating domains (RA1 and RA2) from phospholipase C epsilon
Harris, R., Bunney, T.D., Katan, M., Driscoll, P.C.
J. Biomol. NMR (2005), 33, 138-138, PubMed 16258837 , DOI 10.1007/s10858-005-3094-0 , Abstract

Related entities 1. RA2, : 1 : 3 : 10 entities Detail

Interaction partners 1. RA2, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 14 experiments Detail

1 1H15N HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

2 13C1H HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

3 HNCA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

4 HNcoCA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

5 HNCACB

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

6 CBCAcoNH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

7 HNCO

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

8 HAcacoNH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

9 HCCH-TOCSY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

10 13C NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

11 15N NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

12 NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

13 TOCSY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

14 HACANH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian UnityPlus (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 15N labelled RA2

#	Name	Isotope labeling	Concentration
1	RA2	[U-15N]	1 mM
2	sodium chloride		250 mM
3	DTT		5 mM
4	sodium phosphate		25 mM
5	EDTA		0.1 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details 13C/15N labelled RA2

#	Name	Isotope labeling	Concentration
6	RA2	[U13C; U-15N]	1 mM
7	sodium chloride		250 mM
8	DTT		5 mM
9	sodium phospahte		25 mM
10	EDTA		0.1 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.5 (±0.2), Details unlabelled RA2 in D2O buffer

#	Name	Isotope labeling	Concentration
11	RA2	unlabeled	1 mM
12	sodium chloride		250 mM
13	DTT		5 mM
14	sodium phospahte		25 mM
15	EDTA		0.1 mM
16	dueterium oxide		100 %

null

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Found 1 Document (1.88 seconds)

BMRB: 6635

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RA2, : 1 : 3 : 10 entities Detail

Interaction partners 1. RA2, : 5 interactors Detail

Experiments performed 14 experiments Detail

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