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BMRB: 6644

2MUI

1H, 13C, and 15N NMR assignments for AlgH, a putative transcriptional regulator from Pseudomonas aeruginosa

Authors

Cowley, A.B., Bieber Urbauer, R.J., Urbauer, J.L.

Assembly

assembly AlgH

Entity

1. assembly AlgH (polymer, Thiol state: all free), 189 monomers, 20211.70 Da Detail

MKQSSPTYLK HHFLIAMPHM ADPNFAQTVT YLVEHNEQGA MGLVINRPSG LNLAEVLEQL KPDALPPARC QHIDIYNGGP VQTDRGFVLH PSGLSYQSTL ELGELAMSTS QDVLFAIAAG TGPEKSLISL GYAGWEAGQL EAELSDNAWL TCPADPAILF DLPPEERLSA AAARLGVNLS LLTAQAGHA

Formula weight

20211.7 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 97.0 %, Completeness (bb): 98.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.0 % (2043 of 2106)	96.4 % (1043 of 1082)	97.6 % (810 of 830)	97.9 % (190 of 194)
Backbone	98.9 % (1094 of 1106)	98.9 % (375 of 379)	98.7 % (545 of 552)	99.4 % (174 of 175)
Sidechain	95.4 % (1120 of 1174)	95.0 % (668 of 703)	96.5 % (436 of 452)	84.2 % (16 of 19)
Aromatic	93.0 % (132 of 142)	94.4 % (67 of 71)	94.2 % (65 of 69)	0.0 % (0 of 2)
Methyl	99.6 % (233 of 234)	100.0 % (117 of 117)	99.1 % (116 of 117)

1. AlgH

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.0 (±0.02)

#	Name	Isotope labeling	Concentration
1	AlgH	[U-99% 13C; U-99% 15N]	1.3 mM
2	potassium phosphate		10 mM
3	KCl		10 mM
4	DTT		5 mM
5	D2O		8 %
6	H2O		92 %
7	Sodium azide		0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MUI, Strand ID: A Detail

Release date

2015-04-12

Citation

1H, 13C and 15N NMR assignments for AlgH, a putative transcriptional regulator from Pseudomonas aeruginosa
Cowley, A.B., Bieber Urbauer, R.J., Urbauer, J.L.
J. Biomol. NMR (2005), 33, 74-74, PubMed 16222564 , DOI 10.1007/s10858-005-1271-9 , Abstract

Related entities 1. assembly AlgH, : 1 : 1 : 1 : 105 entities Detail

Experiments performed 11 experiments Detail

1 HNCO