BMRB: 6652

RNA helical packing in solution: NMR structure of a 30 kDa GAAA tetraloop receptor complex

Authors

Davis, J.H., Tonelli, M., Scott, L.G., James, W.R., Butcher, S.E.

Assembly

GAAA tetraloop/Recptor homodimer

Entity

1. GAAA tetraloop/Recptor homodimer (polymer, Thiol state: not present), 43 monomers, 13936.23 × 2 Da Detail

GGGAUAUGGA AGAACCGGGG AAACUUGGUU CUUCCUAAGU CCU

Total weight

27872.46 Da

Max. entity weight

13936.23 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 46.6 %, Completeness (bb): 27.5 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	46.6 % (318 of 683)	55.8 % (207 of 371)	30.6 % (88 of 288)	95.8 % (23 of 24)
Suger, PO4	27.5 % (130 of 473)	36.8 % (95 of 258)	16.3 % (35 of 215)
Nucleobase	89.5 % (188 of 210)	99.1 % (112 of 113)	72.6 % (53 of 73)	95.8 % (23 of 24)
Aromatic	88.7 % (172 of 194)	99.0 % (96 of 97)	72.6 % (53 of 73)	95.8 % (23 of 24)

1. GAAA tetraloop 11-nt receptor RNA

GGGAUAUGGA AGAACCGGGG AAACUUGGUU CUUCCUAAGU CCU

Show sample condition

Sample

Temperature 303 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	GAAA tetraloop 11-nt receptor RNA	[U-95% 13C; U-95% 15N]		1.2 mM
2	pf1 phage			17 (±2.0) mM

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RDC

12 RDC values in 1 lists
Field strength (¹H) 600.13 MHz, Temperature 303 (±1) K Detail

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Temperature 303 (±1) K

List #1 RDC_list_1, RDC code 1DHNN, Field strength (¹H) 600.13 MHz

Release date

2005-09-07

Citation

RNA helical packing in solution: NMR structure of a 30 kDa GAAA tetraloop-receptor complex
Davis, J.H., Tonelli, M., Scott, L.G., Jaeger, L., Williamson, J.R., Butcher, S.E.
J. Mol. Biol. (2005), 351, 371-382, PubMed 16002091 , DOI 10.1016/j.jmb.2005.05.069 , Abstract

Related entities 1. GAAA tetraloop/Recptor homodimer, : 1 : 5 entities Detail

Experiments performed 9 experiments Detail