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Found 1 Document (1.956 seconds)

BMRB: 6655

1ZU1

Solution Structure of the N-terminal Zinc Fingers of the Xenopus laevis double stranded RNA binding protein ZFa

Authors

Wright, P.E., Moller, H.M., Martinez-Yamout, M.A., Dyson, H.JANE.

Assembly

Zinc finger domains 1 and 2 of dsRBP-ZFa

Entity

1. dsRBP-ZFa (polymer, Thiol state: free and other bound), 127 monomers, 14048.72 Da Detail

ADEFGNGDAL DLPVGKDAVN SLIRENSHIF SDTQCKVCSA VLISESQKLA HYQSRKHANK VRRYMAINQG EDSVPAKKFK AAPAEISDGE DRSKCCPVCN MTFSSPVVAE SHYIGKTHIK NLRLREQ

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

14179.538 Da

Max. entity weight

14048.72 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS35:SG	2:ZN1:ZN
2	na	sing	1:CYS38:SG	2:ZN1:ZN
3	na	sing	1:HIS51:NE2	2:ZN1:ZN
4	na	sing	1:HIS57:ND1	2:ZN1:ZN
5	na	sing	1:CYS96:SG	2:ZN1:ZN
6	na	sing	1:CYS99:SG	2:ZN1:ZN
7	na	sing	1:HIS112:NE2	2:ZN1:ZN
8	na	sing	1:HIS118:ND1	2:ZN1:ZN

Source organism

Xenopus laevis

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.6 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.6 % (1365 of 1443)	93.2 % (704 of 755)	95.5 % (529 of 554)	98.5 % (132 of 134)
Backbone	97.3 % (732 of 752)	97.3 % (248 of 255)	97.1 % (364 of 375)	98.4 % (120 of 122)
Sidechain	92.9 % (754 of 812)	91.2 % (456 of 500)	95.3 % (286 of 300)	100.0 % (12 of 12)
Aromatic	66.7 % (56 of 84)	66.7 % (28 of 42)	66.7 % (28 of 42)
Methyl	100.0 % (122 of 122)	100.0 % (61 of 61)	100.0 % (61 of 61)

1. dsRBP-ZFa

ADEFGNGDAL DLPVGKDAVN SLIRENSHIF SDTQCKVCSA VLISESQKLA HYQSRKHANK VRRYMAINQG EDSVPAKKFK AAPAEISDGE DRSKCCPVCN MTFSSPVVAE SHYIGKTHIK NLRLREQ

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBP-ZFa	[U-98% 15N]	protein	1 (±0.2) mM
2	Zinc ion		ligand	2 (±0.4) mM
3	H2O		solvent	95 %
4	D2O		solvent	5 %
5	NaCl		salt	100 mM
6	Tris		buffer	25 mM
7	DTT		reducing agent	2 mM

Sample #2

Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
8	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
9	Zinc ion		ligand	2 (±0.4) mM
10	H2O		solvent	95 %
11	D2O		solvent	5 %
12	NaCl		salt	100 mM
13	Tris		buffer	25 mM
14	DTT		reducing agent	2 mM

Sample #3

Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in D2O

#	Name	Isotope labeling	Type	Concentration
15	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
16	Zinc ion		ligand	2 (±0.4) mM
17	D2O		solvent	100 %
18	NaCl		salt	100 mM
19	Tris		buffer	25 mM
20	DTT		reducing agent	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1ZU1, Strand ID: A Detail

Release date

2005-10-31

Citation

Solution structure of the N-terminal zinc fingers of the Xenopus laevis double-stranded RNA-binding protein ZFa
Moller, H.M., Martinez-Yamout, M.A., Dyson, H.JANE., Wright, P.E.
J. Mol. Biol. (2005), 351, 718-730, PubMed 16051273 , DOI 10.1016/j.jmb.2005.06.032 , Abstract

Related entities 1. dsRBP-ZFa, : 1 : 1 : 17 entities Detail

Experiments performed 16 experiments Detail

1 1H15N_HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBP-ZFa	[U-98% 15N]	protein	1 (±0.2) mM
2	Zinc ion		ligand	2 (±0.4) mM
3	H2O		solvent	95 %
4	D2O		solvent	5 %
5	NaCl		salt	100 mM
6	Tris		buffer	25 mM
7	DTT		reducing agent	2 mM

2 3D_1H15N_TOCSY_HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBP-ZFa	[U-98% 15N]	protein	1 (±0.2) mM
2	Zinc ion		ligand	2 (±0.4) mM
3	H2O		solvent	95 %
4	D2O		solvent	5 %
5	NaCl		salt	100 mM
6	Tris		buffer	25 mM
7	DTT		reducing agent	2 mM

3 3D_1H15N_NOESY_HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBP-ZFa	[U-98% 15N]	protein	1 (±0.2) mM
2	Zinc ion		ligand	2 (±0.4) mM
3	H2O		solvent	95 %
4	D2O		solvent	5 %
5	NaCl		salt	100 mM
6	Tris		buffer	25 mM
7	DTT		reducing agent	2 mM

4 1H15N_2J_HMQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBP-ZFa	[U-98% 15N]	protein	1 (±0.2) mM
2	Zinc ion		ligand	2 (±0.4) mM
3	H2O		solvent	95 %
4	D2O		solvent	5 %
5	NaCl		salt	100 mM
6	Tris		buffer	25 mM
7	DTT		reducing agent	2 mM

5 3D_HNHA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	dsRBP-ZFa	[U-98% 15N]	protein	1 (±0.2) mM
2	Zinc ion		ligand	2 (±0.4) mM
3	H2O		solvent	95 %
4	D2O		solvent	5 %
5	NaCl		salt	100 mM
6	Tris		buffer	25 mM
7	DTT		reducing agent	2 mM

6 1H13C_HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in D2O

#	Name	Isotope labeling	Type	Concentration
15	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
16	Zinc ion		ligand	2 (±0.4) mM
17	D2O		solvent	100 %
18	NaCl		salt	100 mM
19	Tris		buffer	25 mM
20	DTT		reducing agent	2 mM

7 3D_HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
8	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
9	Zinc ion		ligand	2 (±0.4) mM
10	H2O		solvent	95 %
11	D2O		solvent	5 %
12	NaCl		salt	100 mM
13	Tris		buffer	25 mM
14	DTT		reducing agent	2 mM

8 3D_CBCACONH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
8	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
9	Zinc ion		ligand	2 (±0.4) mM
10	H2O		solvent	95 %
11	D2O		solvent	5 %
12	NaCl		salt	100 mM
13	Tris		buffer	25 mM
14	DTT		reducing agent	2 mM

9 3D_HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
8	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
9	Zinc ion		ligand	2 (±0.4) mM
10	H2O		solvent	95 %
11	D2O		solvent	5 %
12	NaCl		salt	100 mM
13	Tris		buffer	25 mM
14	DTT		reducing agent	2 mM

10 3D_HCCH_COSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in D2O

#	Name	Isotope labeling	Type	Concentration
15	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
16	Zinc ion		ligand	2 (±0.4) mM
17	D2O		solvent	100 %
18	NaCl		salt	100 mM
19	Tris		buffer	25 mM
20	DTT		reducing agent	2 mM

11 3D_HCCH_TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in D2O

#	Name	Isotope labeling	Type	Concentration
15	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
16	Zinc ion		ligand	2 (±0.4) mM
17	D2O		solvent	100 %
18	NaCl		salt	100 mM
19	Tris		buffer	25 mM
20	DTT		reducing agent	2 mM

12 3D_1H13C_NOESY_HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in D2O

#	Name	Isotope labeling	Type	Concentration
15	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
16	Zinc ion		ligand	2 (±0.4) mM
17	D2O		solvent	100 %
18	NaCl		salt	100 mM
19	Tris		buffer	25 mM
20	DTT		reducing agent	2 mM

13 3D_HACAHB_COSY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in D2O

#	Name	Isotope labeling	Type	Concentration
15	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
16	Zinc ion		ligand	2 (±0.4) mM
17	D2O		solvent	100 %
18	NaCl		salt	100 mM
19	Tris		buffer	25 mM
20	DTT		reducing agent	2 mM

14 13C13CO_SED_CT_HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in D2O

#	Name	Isotope labeling	Type	Concentration
15	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
16	Zinc ion		ligand	2 (±0.4) mM
17	D2O		solvent	100 %
18	NaCl		salt	100 mM
19	Tris		buffer	25 mM
20	DTT		reducing agent	2 mM

15 13C15N_SED_CT_HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in D2O

#	Name	Isotope labeling	Type	Concentration
15	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
16	Zinc ion		ligand	2 (±0.4) mM
17	D2O		solvent	100 %
18	NaCl		salt	100 mM
19	Tris		buffer	25 mM
20	DTT		reducing agent	2 mM

16 3D_HBHACBCACONH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 298 (±0.5) K, pH 6.9 (±0.1), Details Uniformly 15N/13C-labeled protein in H2O/D2O

#	Name	Isotope labeling	Type	Concentration
8	dsRBP-ZFa	[U-98% 15N; U-98% 13C]	protein	1 (±0.2) mM
9	Zinc ion		ligand	2 (±0.4) mM
10	H2O		solvent	95 %
11	D2O		solvent	5 %
12	NaCl		salt	100 mM
13	Tris		buffer	25 mM
14	DTT		reducing agent	2 mM

nullKeywords helix-loop-helix, helix-turn-helix, JAZ, NMR, PAG608, wig-1

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Found 1 Document (1.956 seconds)

BMRB: 6655

Sample #1

Sample #2

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. dsRBP-ZFa, : 1 : 1 : 17 entities Detail

Experiments performed 16 experiments Detail

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