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BMRB: 6682

1ZR9

Solution Structure of a Human C2H2-type Zinc Finger Protein

Authors

Lytle, B.L., Peterson, F.C., Volkman, B.F.

Assembly

Zinc finger protein 593

Entity

1. Zinc finger protein 593 (polymer, Thiol state: all other bound), 124 monomers, 14159.64 Da Detail

GHHHHHHLEK AKRRRPDLDE IHRELRPQGS ARPQPDPNAE FDPDLPGGGL HRCLACARYF IDSTNLKTHF RSKDHKKRLK QLSVEPYSQE EAERAAGMGS YVPPRRLAVP TEVSTEVPEM DTST

2. ZN (non-polymer), 65.409 Da

Total weight

14225.049 Da

Max. entity weight

14159.64 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS53:SG	2:ZN1:ZN
2	na	sing	1:CYS56:SG	2:ZN1:ZN
3	na	sing	1:HIS69:NE2	2:ZN1:ZN
4	na	sing	1:HIS75:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Automated methods were used for backbone chemical shift assignment and iterative NOE refinement. Final structures were obtained by molecular dynamics in explicit solvent.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 64.5 %, Completeness: 56.1 %, Completeness (bb): 60.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	56.1 % (803 of 1431)	55.7 % (423 of 759)	55.8 % (309 of 554)	60.2 % (71 of 118)
Backbone	60.1 % (433 of 720)	62.6 % (152 of 243)	58.6 % (214 of 365)	59.8 % (67 of 112)
Sidechain	53.0 % (439 of 828)	52.7 % (272 of 516)	53.3 % (163 of 306)	66.7 % (4 of 6)
Aromatic	51.1 % (48 of 94)	61.7 % (29 of 47)	40.4 % (19 of 47)
Methyl	53.1 % (52 of 98)	53.1 % (26 of 49)	53.1 % (26 of 49)

1. Zinc finger protein 593

GHHHHHHLEK AKRRRPDLDE IHRELRPQGS ARPQPDPNAE FDPDLPGGGL HRCLACARYF IDSTNLKTHF RSKDHKKRLK QLSVEPYSQE EAERAAGMGS YVPPRRLAVP TEVSTEVPEM DTST

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Zinc finger protein 593	[U-15N; U-13C]	0.6 mM
2	Bis Tris		10 mM
3	NaCl		100 mM
4	DTT		2 mM
5	H2O		90 %
6	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1ZR9, Strand ID: A Detail

Release date

2005-07-26

Citation

The solution structure of ZNF593 from Homo sapiens reveals a zinc finger in a predominantly unstructured protein
Hayes, P.L., Lytle, B.L., Volkman, B.F., Peterson, F.C.
Protein Sci. (2008), 17, 571-576, PubMed 18287285 , DOI 10.1110/ps.073290408 , Abstract

Related entities 1. Zinc finger protein 593, : 1 : 2 : 36 entities Detail

Interaction partners 1. Zinc finger protein 593, : 6 interactors Detail

More details for 6 interactors identified by 4 citations

Experiments performed 12 experiments Detail

1 1H-15N HSQC