BMRBj - BMREntryMaster

Found 1 Document (2.056 seconds)

BMRB: 6690

2BN5

Partial 1H, 13C, and 15N Chemical Shift Assignments for PSI AB box region in complex with U1-70k protein proline-rich peptide

Authors

Ignjatovic, T., Yang, J., Butler, J., Neuhaus, D., Nagai, K.

Assembly

PSI AB complex with U1-70k proline-rich peptide

Entity

1. PSI AB box (polymer, Thiol state: not present), 93 monomers, 10374.11 Da Detail

SDYSAQWIEY YKQMGSHREA EMIEQQMKAK QAGGSSGPVQ QQQPQQQQQS QQQTGGAGAG GADYSAQWAE YYRSVGKIEE AEAIEKTLKN KQN

2. U1-70k peptide (polymer, Thiol state: not present), 21 monomers, 2274.645 Da Detail

RPPPAHHNMF SVPPPPILGR G

Total weight

12648.756 Da

Max. entity weight

10374.11 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

SIMULATED ANNEAING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 64.0 %, Completeness: 34.5 %, Completeness (bb): 44.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	34.5 % (456 of 1320)	34.1 % (240 of 704)	31.4 % (153 of 487)	48.8 % (63 of 129)
Backbone	44.0 % (293 of 666)	51.1 % (118 of 231)	33.9 % (112 of 330)	60.0 % (63 of 105)
Sidechain	27.0 % (204 of 756)	25.8 % (122 of 473)	31.7 % (82 of 259)	0.0 % (0 of 24)
Aromatic	37.2 % (35 of 94)	40.4 % (19 of 47)	35.6 % (16 of 45)	0.0 % (0 of 2)
Methyl	52.9 % (36 of 68)	55.9 % (19 of 34)	50.0 % (17 of 34)

1. PSI AB box

SDYSAQWIEY YKQMGSHREA EMIEQQMKAK QAGGSSGPVQ QQQPQQQQQS QQQTGGAGAG GADYSAQWAE YYRSVGKIEE AEAIEKTLKN KQN

2. U1-70k peptide

RPPPAHHNMF SVPPPPILGR G

Show sample condition

Sample

Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

Protein Blocks Logo

Calculated from 28 models in PDB: 2BN5, Strand ID: A, B Detail

Release date

2007-01-25

Citation

Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase
Ignjatovic, T., Yang, J., Butler, J., Neuhaus, D., Nagai, K.
J. Mol. Biol. (2005), 351, 52-65, PubMed 15990112 , DOI 10.1016/j.jmb.2005.04.077 , Abstract

Related entities 1. PSI AB box, : 1 entities Detail

Related entities 2. U1-70k peptide, : 2 : 6 entities Detail

Interaction partners 2. U1-70k peptide, : 6 interactors Detail

More details for 6 interactors identified by 2 citations

Experiments performed 16 experiments Detail

1 2D NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N labelled complex

#	Name	Isotope labeling	Concentration
1	PSI AB box	[U-15N]	0.4 ~ 0.42 mM
2	U1-70k peptide		0.4 ~ 0.42 mM
3	sodium phosphate		10 mM
4	sodium chloride		150 mM
5	DTT		5 mM

2 2D TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N labelled complex

#	Name	Isotope labeling	Concentration
1	PSI AB box	[U-15N]	0.4 ~ 0.42 mM
2	U1-70k peptide		0.4 ~ 0.42 mM
3	sodium phosphate		10 mM
4	sodium chloride		150 mM
5	DTT		5 mM

3 2D 15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N labelled complex

#	Name	Isotope labeling	Concentration
1	PSI AB box	[U-15N]	0.4 ~ 0.42 mM
2	U1-70k peptide		0.4 ~ 0.42 mM
3	sodium phosphate		10 mM
4	sodium chloride		150 mM
5	DTT		5 mM

4 2D 13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

5 3D HNCA

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

6 3D HN(CO)CA

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

7 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

8 3D CBCANH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

9 3D HBHA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

10 3D HBHANH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

11 3D HCCH COSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

12 3D HCCH TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

13 3D 15N NOESY-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N labelled complex

#	Name	Isotope labeling	Concentration
1	PSI AB box	[U-15N]	0.4 ~ 0.42 mM
2	U1-70k peptide		0.4 ~ 0.42 mM
3	sodium phosphate		10 mM
4	sodium chloride		150 mM
5	DTT		5 mM

14 3D 13C NOESY-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

15 2D (13C, 15N) Filtered NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

16 3D (13C, 15N) Filtered 13C HSQC NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 290 (±0.5) K, pH 6.5 (±0.1), Details 15N, 13C labelled complex

#	Name	Isotope labeling	Concentration
6	PSI AB box	[U-13C; U-15N]	0.4 ~ 0.45 mM
7	U1-70k peptide		0.4 ~ 0.45 mM
8	sodium phosphate		10 mM
9	sodium chloride		150 mM
10	DTT		5 mM

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Found 1 Document (2.056 seconds)

BMRB: 6690

Sample

Related entities 1. PSI AB box, : 1 entities Detail

Related entities 2. U1-70k peptide, : 2 : 6 entities Detail

Interaction partners 2. U1-70k peptide, : 6 interactors Detail

Experiments performed 16 experiments Detail

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