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BMRB: 6696

2BUG

G83N TPR domain of Ppp5

Authors

Cliff, M.J., Harris, R., Barford, D., Ladbury, J.E., Williams, M.A.

Assembly

PP5-TPR-peptide complex

Entity

1. tetratricopeptide domain from Ppp5 (polymer, Thiol state: all free), 140 monomers, 16179.12 Da Detail

MSGHHHHHHT DPPADGALKR AEELKTQAND YFKAKDYENA IKFYSQAIEL NPSNAIYYGN RSLAYLRTEC YGYALNDATR AIELDKKYIK GYYRRAASNM ALGKFRAALR DYETVVKVKP HDKDAKMKYQ ECNKIVKQKA

2. tetratricopeptide domain from Ppp5 (polymer, Thiol state: not present), 6 monomers, 647.6946 Da Detail

XMEEVD

Total weight

16826.814 Da

Max. entity weight

16179.12 Da

Source organism

Exptl. method

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.2 %, Completeness: 87.4 %, Completeness (bb): 87.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.4 % (1506 of 1724)	88.5 % (800 of 904)	85.5 % (570 of 667)	88.9 % (136 of 153)
Backbone	87.6 % (755 of 862)	90.1 % (263 of 292)	85.3 % (366 of 429)	89.4 % (126 of 141)
Sidechain	87.3 % (874 of 1001)	87.7 % (537 of 612)	86.7 % (327 of 377)	83.3 % (10 of 12)
Aromatic	82.1 % (133 of 162)	82.7 % (67 of 81)	81.5 % (66 of 81)
Methyl	98.4 % (126 of 128)	100.0 % (64 of 64)	96.9 % (62 of 64)

1. tetratricopeptide domain from Ppp5

MSGHHHHHHT DPPADGALKR AEELKTQAND YFKAKDYENA IKFYSQAIEL NPSNAIYYGN RSLAYLRTEC YGYALNDATR AIELDKKYIK GYYRRAASNM ALGKFRAALR DYETVVKVKP HDKDAKMKYQ ECNKIVKQKA

2. tetratricopeptide domain from Ppp5

XMEEVD

Show sample condition

Sample

Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

Protein Blocks Logo

Calculated from 19 models in PDB: 2BUG, Strand ID: A, B Detail

Release date

2006-04-04

Citation

Conformational diversity in the TPR domain-mediated interaction of protein phosphatase 5 with Hsp90
Cliff, M.J., Harris, R., Barford, D., Ladbury, J.E., Williams, M.A.
Structure (2006), 14, 415-426, PubMed 16531226 , DOI 10.1016/j.str.2005.12.009 , Abstract

Related entities 1. tetratricopeptide domain from Ppp5, : 76 entities Detail

Experiments performed 10 experiments Detail

1 1H15NHSQC

Instrument

Varian Unity Inova - 800 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

2 1H13CHSQC

Instrument

Varian Unity Inova - 800 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

3 HNCA

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

4 CBCACONH

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

5 HNHB

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

6 1H15NTOWNY HSQC

Instrument

Varian Unity Inova - 800 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

7 1H15NNOESY HSQC

Instrument

Varian Unity Inova - 800 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

8 HNCO

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

9 1H13CNOESY HSQC

Instrument

Varian Unity Inova - 800 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

10 HCCH-TOCSY-HSQC

Instrument

Varian Unity Inova - 600 MHz

Sample

State isotropic, Pressure 1 (±0.1) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details pH 6.0 reducing conditions

#	Name	Isotope labeling	Type	Concentration
1	tetratricopeptide domain from Ppp5	[U-13C; U-15N]	protein	0.75 (±0.4) mM
2	Hsp90 pentapeptide		ligand	0.75 (±0.4) mM
3	MES		buffer	50 (±0.2) mM
4	DTT		reducing agent	5 (±0.1) mM

nullKeywords NMR assigment